Cyt1Aa protein from Bacillus thuringiensis (Berliner) serovar israelensis is active against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann)
BACKGROUND: Ceratitis capitata (Wiedemann) is one of the world's most destructive fruit pests. The aim of this study was to ascertain insecticidal activity of Bacillus thuringiensis (Berliner) delta-endotoxins to C. capitata. RESULTS: Among 42 selected Bacillus strains, only B. thuringiensis ser...
| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5052 |
| _version_ | 1855032217953107968 |
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| author | Cristian Vidal-Quist, J. Castanera, Pedro González-Cabrera, Joel |
| author_browse | Castanera, Pedro Cristian Vidal-Quist, J. González-Cabrera, Joel |
| author_facet | Cristian Vidal-Quist, J. Castanera, Pedro González-Cabrera, Joel |
| author_sort | Cristian Vidal-Quist, J. |
| collection | ReDivia |
| description | BACKGROUND: Ceratitis capitata (Wiedemann) is one of the world's most destructive fruit pests. The aim of this study was to ascertain insecticidal activity of Bacillus thuringiensis (Berliner) delta-endotoxins to C. capitata. RESULTS: Among 42 selected Bacillus strains, only B. thuringiensis serovar israelensis (Bti) solubilised protoxins showed biological activity against C. capitata neonate larvae, whereas Bti spore and crystal mixture was inactive. Insecticidal activity of Bti protoxins was significantly enhanced by incubation with Culex pipiens L. gut extracts. Overdigestion of Bti protoxins with Sesamia nonagrioides (Lefebvre) gut extracts suppressed biological activity against C. capitata, and this correlated with degradation of Cyt toxins. Cyt1Aa solubilised protoxin showed the highest toxicity, LC50 after 7 days of 4.93 mu g cm(-2), while proteolytical processing of Cyt1Aa protoxins by larval gut extracts did not enhance insecticidal activity. CONCLUSION: The present study provides evidence for the first time of the insecticidal activity of a B. thuringiensis strain against C. capitata and identifies a single delta-endotoxin with potential for controlling this pest. (C) 2010 Society of Chemical Industry |
| format | article |
| id | ReDivia5052 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia50522025-04-25T14:45:15Z Cyt1Aa protein from Bacillus thuringiensis (Berliner) serovar israelensis is active against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) Cristian Vidal-Quist, J. Castanera, Pedro González-Cabrera, Joel BACKGROUND: Ceratitis capitata (Wiedemann) is one of the world's most destructive fruit pests. The aim of this study was to ascertain insecticidal activity of Bacillus thuringiensis (Berliner) delta-endotoxins to C. capitata. RESULTS: Among 42 selected Bacillus strains, only B. thuringiensis serovar israelensis (Bti) solubilised protoxins showed biological activity against C. capitata neonate larvae, whereas Bti spore and crystal mixture was inactive. Insecticidal activity of Bti protoxins was significantly enhanced by incubation with Culex pipiens L. gut extracts. Overdigestion of Bti protoxins with Sesamia nonagrioides (Lefebvre) gut extracts suppressed biological activity against C. capitata, and this correlated with degradation of Cyt toxins. Cyt1Aa solubilised protoxin showed the highest toxicity, LC50 after 7 days of 4.93 mu g cm(-2), while proteolytical processing of Cyt1Aa protoxins by larval gut extracts did not enhance insecticidal activity. CONCLUSION: The present study provides evidence for the first time of the insecticidal activity of a B. thuringiensis strain against C. capitata and identifies a single delta-endotoxin with potential for controlling this pest. (C) 2010 Society of Chemical Industry 2017-06-01T10:11:36Z 2017-06-01T10:11:36Z 2010 SEP 2010 article Cristian Vidal-Quist, J., Castanera, P., Gonzalez-Cabrera, J. (2010). Cyt1Aa protein from Bacillus thuringiensis (Berliner), serovar israelensis is active against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann). Pest management science, 66(9), 949-955. 1526-498X http://hdl.handle.net/20.500.11939/5052 10.1002/ps.1965 en openAccess Impreso |
| spellingShingle | Cristian Vidal-Quist, J. Castanera, Pedro González-Cabrera, Joel Cyt1Aa protein from Bacillus thuringiensis (Berliner) serovar israelensis is active against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) |
| title | Cyt1Aa protein from Bacillus thuringiensis (Berliner) serovar israelensis is active against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) |
| title_full | Cyt1Aa protein from Bacillus thuringiensis (Berliner) serovar israelensis is active against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) |
| title_fullStr | Cyt1Aa protein from Bacillus thuringiensis (Berliner) serovar israelensis is active against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) |
| title_full_unstemmed | Cyt1Aa protein from Bacillus thuringiensis (Berliner) serovar israelensis is active against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) |
| title_short | Cyt1Aa protein from Bacillus thuringiensis (Berliner) serovar israelensis is active against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) |
| title_sort | cyt1aa protein from bacillus thuringiensis berliner serovar israelensis is active against the mediterranean fruit fly ceratitis capitata wiedemann |
| url | http://hdl.handle.net/20.500.11939/5052 |
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