Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration
The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of citrange (Citrus sinensis L. OsbeckxPoncirus trifoliata L. Raf.) have been investigated. Factors such as cocultivation period, preculture of explants, use of acetosy...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5014 |
| _version_ | 1855491852246974464 |
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| author | Cervera, Magdalena Pina, José A. Juárez, José Navarro, Luis Pena, Leandro |
| author_browse | Cervera, Magdalena Juárez, José Navarro, Luis Pena, Leandro Pina, José A. |
| author_facet | Cervera, Magdalena Pina, José A. Juárez, José Navarro, Luis Pena, Leandro |
| author_sort | Cervera, Magdalena |
| collection | ReDivia |
| description | The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of citrange (Citrus sinensis L. OsbeckxPoncirus trifoliata L. Raf.) have been investigated. Factors such as cocultivation period, preculture of explants, use of acetosyringone or feeder plates during cocultivation, cocultivation on a medium rich in auxins, post cultivation in darkness, and different kanamycin concentrations for selection were assessed. A 3-day cocultivation on a medium rich in auxins improved transformation frequencies, since it increased the number of dividing cells competent for transformation, at the cut ends of the explants. Exposure of explants to darkness for 4 weeks on selection medium resulted in further callus development and increased the regeneration frequency of transgenic shoots. Furthermore, this treatment drastically reduced the number of regenerated escape shoots. A transformation efficiency of 41.3% was achieved using the optimized transformation procedure. |
| format | Artículo |
| id | ReDivia5014 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia50142025-04-25T14:45:10Z Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration Cervera, Magdalena Pina, José A. Juárez, José Navarro, Luis Pena, Leandro The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of citrange (Citrus sinensis L. OsbeckxPoncirus trifoliata L. Raf.) have been investigated. Factors such as cocultivation period, preculture of explants, use of acetosyringone or feeder plates during cocultivation, cocultivation on a medium rich in auxins, post cultivation in darkness, and different kanamycin concentrations for selection were assessed. A 3-day cocultivation on a medium rich in auxins improved transformation frequencies, since it increased the number of dividing cells competent for transformation, at the cut ends of the explants. Exposure of explants to darkness for 4 weeks on selection medium resulted in further callus development and increased the regeneration frequency of transgenic shoots. Furthermore, this treatment drastically reduced the number of regenerated escape shoots. A transformation efficiency of 41.3% was achieved using the optimized transformation procedure. 2017-06-01T10:11:33Z 2017-06-01T10:11:33Z 1998 DEC article Cervera, M., Pina, JA, Juarez, J., Navarro, L., Pena, L. (1998). Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration. Plant Cell Reports, 18(3-4), 271-278. 0721-7714 http://hdl.handle.net/20.500.11939/5014 10.1007/s002990050570 en openAccess Impreso |
| spellingShingle | Cervera, Magdalena Pina, José A. Juárez, José Navarro, Luis Pena, Leandro Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration |
| title | Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration |
| title_full | Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration |
| title_fullStr | Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration |
| title_full_unstemmed | Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration |
| title_short | Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration |
| title_sort | agrobacterium mediated transformation of citrange factors affecting transformation and regeneration |
| url | http://hdl.handle.net/20.500.11939/5014 |
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