Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus
Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relati...
| Autores principales: | , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4998 |
| _version_ | 1855032209413505024 |
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| author | Cecilia Zanek, Maria Andrea Reyes, Carina Cervera, Magdalena Pena, Eduardo Jose Velázquez, Karelia Costa, Norma Ines Plata, Maria Grau, Oscar Pena, Leandro Laura Garcia, Maria |
| author_browse | Andrea Reyes, Carina Cecilia Zanek, Maria Cervera, Magdalena Costa, Norma Grau, Oscar Ines Plata, Maria Laura Garcia, Maria Pena, Eduardo Jose Pena, Leandro Velázquez, Karelia |
| author_facet | Cecilia Zanek, Maria Andrea Reyes, Carina Cervera, Magdalena Pena, Eduardo Jose Velázquez, Karelia Costa, Norma Ines Plata, Maria Grau, Oscar Pena, Leandro Laura Garcia, Maria |
| author_sort | Cecilia Zanek, Maria |
| collection | ReDivia |
| description | Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootstocks. Certification programs have not been sufficient to control the disease and no sources of natural resistance have been found. Pathogen-derived resistance (PDR) can provide an efficient alternative to control viral diseases in their hosts. For this purpose, we have produced 21 independent lines of sweet orange expressing the coat protein gene of CPsV and five of them were challenged with the homologous CPV 4 isolate. Two different viral loads were evaluated to challenge the transgenic plants, but so far, no resistance or tolerance has been found in any line after 1 year of observations. In contrast, after inoculation all lines showed characteristic symptoms of psorosis in the greenhouse. The transgenic lines expressed low and variable amounts of the cp gene and no correlation was found between copy number and transgene expression. One line contained three copies of the cp gene, expressed low amounts of the mRNA and no coat protein. The ORF was cytosine methylated suggesting a PTGS mechanism, although the transformant failed to protect against the viral load used. Possible causes for the failed protection against the CPsV are discussed. |
| format | article |
| id | ReDivia4998 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia49982025-04-25T14:45:06Z Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus Cecilia Zanek, Maria Andrea Reyes, Carina Cervera, Magdalena Pena, Eduardo Jose Velázquez, Karelia Costa, Norma Ines Plata, Maria Grau, Oscar Pena, Leandro Laura Garcia, Maria Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootstocks. Certification programs have not been sufficient to control the disease and no sources of natural resistance have been found. Pathogen-derived resistance (PDR) can provide an efficient alternative to control viral diseases in their hosts. For this purpose, we have produced 21 independent lines of sweet orange expressing the coat protein gene of CPsV and five of them were challenged with the homologous CPV 4 isolate. Two different viral loads were evaluated to challenge the transgenic plants, but so far, no resistance or tolerance has been found in any line after 1 year of observations. In contrast, after inoculation all lines showed characteristic symptoms of psorosis in the greenhouse. The transgenic lines expressed low and variable amounts of the cp gene and no correlation was found between copy number and transgene expression. One line contained three copies of the cp gene, expressed low amounts of the mRNA and no coat protein. The ORF was cytosine methylated suggesting a PTGS mechanism, although the transformant failed to protect against the viral load used. Possible causes for the failed protection against the CPsV are discussed. 2017-06-01T10:11:31Z 2017-06-01T10:11:31Z 2008 JAN 2008 article Cecilia Zanek, M., Andrea Reyes, Carina, Cervera, M., J. Pena, E., Velazquez, Karelia, Costa, Norma, Ines Plata, M., Grau, O., Pena, L., L. Garcia, M. (2008). Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus. Plant Cell Reports, 27(1), 57-66. 0721-7714 http://hdl.handle.net/20.500.11939/4998 10.1007/s00299-007-0422-8 en openAccess Impreso |
| spellingShingle | Cecilia Zanek, Maria Andrea Reyes, Carina Cervera, Magdalena Pena, Eduardo Jose Velázquez, Karelia Costa, Norma Ines Plata, Maria Grau, Oscar Pena, Leandro Laura Garcia, Maria Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus |
| title | Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus |
| title_full | Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus |
| title_fullStr | Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus |
| title_full_unstemmed | Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus |
| title_short | Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus |
| title_sort | genetic transformation of sweet orange with the coat protein gene of citrus psorosis virus and evaluation of resistance against the virus |
| url | http://hdl.handle.net/20.500.11939/4998 |
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