Identification of transcription factors potentially involved in the juvenile to adult phase transition in Citrus
The juvenile to adult transition (JAT) in higher plants is required for them to reach reproductive competence. However, it is a poorly understood process in woody plants, where only a few genes have been definitely identified as being involved in this transition. This work aims at increasing our und...
| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4985 |
| _version_ | 1855032207120269312 |
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| author | Castillo, Mari-Cruz Forment, Javier Gadea, Jose Carrasco, José L. Juárez, José Navarro, Luis Ancillo, Gema |
| author_browse | Ancillo, Gema Carrasco, José L. Castillo, Mari-Cruz Forment, Javier Gadea, Jose Juárez, José Navarro, Luis |
| author_facet | Castillo, Mari-Cruz Forment, Javier Gadea, Jose Carrasco, José L. Juárez, José Navarro, Luis Ancillo, Gema |
| author_sort | Castillo, Mari-Cruz |
| collection | ReDivia |
| description | The juvenile to adult transition (JAT) in higher plants is required for them to reach reproductive competence. However, it is a poorly understood process in woody plants, where only a few genes have been definitely identified as being involved in this transition. This work aims at increasing our understanding of the mechanisms regulating the JAT in citrus. Juvenile and adult plants from Pineapple sweet orange (Citrus sinensis) and Rough lemon (C. jambhiri) were used to screen for differentially expressed transcription factors (TFs) using a 115K microarray developed on the basis of the CitrusTF database. Murcott tangor (C. reticulata C. sinensis) and Duncan grapefruit (C. paradisi) were incorporated into the quantitative real-time reverse transcriptionPCR validation in order to select those genes whose phase-specific regulation was common to the four species. A browsable web database has been created with information about the structural and functional annotation related to 1152 unigenes of putative citrus TFs (CTFs). This database constitutes a valuable resource for research on transcriptional regulation and comparative genomics. Moreover, a microarray has been developed and used that contains these putative CTFs, in order to identify eight genes that showed differential expression in juvenile and adult meristems of four different species of citrus. Those genes have been characterized, and their expression pattern in vegetative and reproductive tissues has been analysed. Four of them are MADS-box genes, a family of TFs involved in developmental processes, whereas another one resembles MADS-box genes but lacks the MADS box itself. The other three showed high partial sequence similarity restricted to specific Arabidopsis protein domains but negligible outside those domains. The work presented here indicates that the JAT in citrus could be controlled by mechanisms that are in part common to those of Arabidopsis, but also somehow different, since specific factors without Arabidopsis orthologues have also been characterized. The potential involvement of the genes in the JAT is discussed. |
| format | article |
| id | ReDivia4985 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia49852025-04-25T14:45:05Z Identification of transcription factors potentially involved in the juvenile to adult phase transition in Citrus Castillo, Mari-Cruz Forment, Javier Gadea, Jose Carrasco, José L. Juárez, José Navarro, Luis Ancillo, Gema The juvenile to adult transition (JAT) in higher plants is required for them to reach reproductive competence. However, it is a poorly understood process in woody plants, where only a few genes have been definitely identified as being involved in this transition. This work aims at increasing our understanding of the mechanisms regulating the JAT in citrus. Juvenile and adult plants from Pineapple sweet orange (Citrus sinensis) and Rough lemon (C. jambhiri) were used to screen for differentially expressed transcription factors (TFs) using a 115K microarray developed on the basis of the CitrusTF database. Murcott tangor (C. reticulata C. sinensis) and Duncan grapefruit (C. paradisi) were incorporated into the quantitative real-time reverse transcriptionPCR validation in order to select those genes whose phase-specific regulation was common to the four species. A browsable web database has been created with information about the structural and functional annotation related to 1152 unigenes of putative citrus TFs (CTFs). This database constitutes a valuable resource for research on transcriptional regulation and comparative genomics. Moreover, a microarray has been developed and used that contains these putative CTFs, in order to identify eight genes that showed differential expression in juvenile and adult meristems of four different species of citrus. Those genes have been characterized, and their expression pattern in vegetative and reproductive tissues has been analysed. Four of them are MADS-box genes, a family of TFs involved in developmental processes, whereas another one resembles MADS-box genes but lacks the MADS box itself. The other three showed high partial sequence similarity restricted to specific Arabidopsis protein domains but negligible outside those domains. The work presented here indicates that the JAT in citrus could be controlled by mechanisms that are in part common to those of Arabidopsis, but also somehow different, since specific factors without Arabidopsis orthologues have also been characterized. The potential involvement of the genes in the JAT is discussed. 2017-06-01T10:11:30Z 2017-06-01T10:11:30Z 2013 NOV article Castillo, Mari-Cruz, Forment, J., Gadea, J., L. Carrasco, J., Juarez, J., Navarro, L., Ancillo, G. (2013). Identification of transcription factors potentially involved in the juvenile to adult phase transition in Citrus. Annals of Botany, 112(7), 1371-1381. 0305-7364 http://hdl.handle.net/20.500.11939/4985 10.1093/aob/mct211 en openAccess Impreso |
| spellingShingle | Castillo, Mari-Cruz Forment, Javier Gadea, Jose Carrasco, José L. Juárez, José Navarro, Luis Ancillo, Gema Identification of transcription factors potentially involved in the juvenile to adult phase transition in Citrus |
| title | Identification of transcription factors potentially involved in the juvenile to adult phase transition in Citrus |
| title_full | Identification of transcription factors potentially involved in the juvenile to adult phase transition in Citrus |
| title_fullStr | Identification of transcription factors potentially involved in the juvenile to adult phase transition in Citrus |
| title_full_unstemmed | Identification of transcription factors potentially involved in the juvenile to adult phase transition in Citrus |
| title_short | Identification of transcription factors potentially involved in the juvenile to adult phase transition in Citrus |
| title_sort | identification of transcription factors potentially involved in the juvenile to adult phase transition in citrus |
| url | http://hdl.handle.net/20.500.11939/4985 |
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