Does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance?
The bioavailability of buserelin acetate when added to the seminal dose appears to be determined by the activity of the existing aminopeptidases. Thus, the addition of amino peptidase inhibitors to rabbit semen extenders could be a solution to decrease the hormone degradation. This study was conduct...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4983 https://www.sciencedirect.com/science/article/abs/pii/S0093691X15006093?via%3Dihub |
| _version_ | 1855032206765850624 |
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| author | Casares-Crespo, Lucía Vicente, José Salvador Talavan, Amparo Viudes-De-Castro, María P. |
| author_browse | Casares-Crespo, Lucía Talavan, Amparo Vicente, José Salvador Viudes-De-Castro, María P. |
| author_facet | Casares-Crespo, Lucía Vicente, José Salvador Talavan, Amparo Viudes-De-Castro, María P. |
| author_sort | Casares-Crespo, Lucía |
| collection | ReDivia |
| description | The bioavailability of buserelin acetate when added to the seminal dose appears to be determined by the activity of the existing aminopeptidases. Thus, the addition of amino peptidase inhibitors to rabbit semen extenders could be a solution to decrease the hormone degradation. This study was conducted to evaluate the effect of the protease activity inhibition on rabbit semen quality parameters and reproductive performance after artificial insemination. Seminal quality was not affected by the incubation with protease inhibitors, being the values of motility, viability, and acrosome integrity not significantly different between the protease inhibitors and the control group. In addition, seminal plasma aminopeptidase activity was inhibited in a 55.1% by the protease inhibitors. On the other hand, regarding the effect of protease inhibitors on reproductive performance, our results showed that the presence of protease inhibitors affected the prolificacy rate (9.2 +/- 0.26 and 9.3 +/- 0.23 vs. 8.2 +/- 0.22 total born per litter for negative control, positive control, and aminopeptidase inhibitors group, respectively; P < 0.05), having this group one kit less per delivery. We conclude that the addition of a wide variety of protease inhibitors in the rabbit semen extender negatively affects prolificacy rate. Therefore, the development of new extenders with specific aminopeptidase inhibitors would be one of the strategies to increase the bioavailability of GnRH analogues without affecting the litter size. |
| format | article |
| id | ReDivia4983 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia49832025-04-25T14:45:05Z Does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance? Casares-Crespo, Lucía Vicente, José Salvador Talavan, Amparo Viudes-De-Castro, María P. The bioavailability of buserelin acetate when added to the seminal dose appears to be determined by the activity of the existing aminopeptidases. Thus, the addition of amino peptidase inhibitors to rabbit semen extenders could be a solution to decrease the hormone degradation. This study was conducted to evaluate the effect of the protease activity inhibition on rabbit semen quality parameters and reproductive performance after artificial insemination. Seminal quality was not affected by the incubation with protease inhibitors, being the values of motility, viability, and acrosome integrity not significantly different between the protease inhibitors and the control group. In addition, seminal plasma aminopeptidase activity was inhibited in a 55.1% by the protease inhibitors. On the other hand, regarding the effect of protease inhibitors on reproductive performance, our results showed that the presence of protease inhibitors affected the prolificacy rate (9.2 +/- 0.26 and 9.3 +/- 0.23 vs. 8.2 +/- 0.22 total born per litter for negative control, positive control, and aminopeptidase inhibitors group, respectively; P < 0.05), having this group one kit less per delivery. We conclude that the addition of a wide variety of protease inhibitors in the rabbit semen extender negatively affects prolificacy rate. Therefore, the development of new extenders with specific aminopeptidase inhibitors would be one of the strategies to increase the bioavailability of GnRH analogues without affecting the litter size. 2017-06-01T10:11:30Z 2017-06-01T10:11:30Z 2016 MAR 15 2016 article acceptedVersion Casares-Crespo, L., Vicente, J.S., Talavan, A. M., Viudes-de-Castro, M.P. (2016). Does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance?. Theriogenology, 85(5), 928-932. 0093-691X http://hdl.handle.net/20.500.11939/4983 10.1016/j.theriogenology.2015.10.044 https://www.sciencedirect.com/science/article/abs/pii/S0093691X15006093?via%3Dihub en openAccess Impreso |
| spellingShingle | Casares-Crespo, Lucía Vicente, José Salvador Talavan, Amparo Viudes-De-Castro, María P. Does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance? |
| title | Does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance? |
| title_full | Does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance? |
| title_fullStr | Does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance? |
| title_full_unstemmed | Does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance? |
| title_short | Does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance? |
| title_sort | does the inclusion of protease inhibitors in the insemination extender affect rabbit reproductive performance |
| url | http://hdl.handle.net/20.500.11939/4983 https://www.sciencedirect.com/science/article/abs/pii/S0093691X15006093?via%3Dihub |
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