Enrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers
Sensitive and specific routine detection of Ralstonia solanacearum in symptomless potato tubers was achieved by efficient enrichment followed by a reliable double-antibody sandwich indirect enzyme-linked immunosorbent assay based on the specific monoclonal antibody 8B-IVIA. This monoclonal antibody...
| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4978 |
| _version_ | 1855032205839958016 |
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| author | Caruso, Paola Gorris, María T. Cambra, Mariano Palomo, José L. Collar, Jesús López, María M. |
| author_browse | Cambra, Mariano Caruso, Paola Collar, Jesús Gorris, María T. López, María M. Palomo, José L. |
| author_facet | Caruso, Paola Gorris, María T. Cambra, Mariano Palomo, José L. Collar, Jesús López, María M. |
| author_sort | Caruso, Paola |
| collection | ReDivia |
| description | Sensitive and specific routine detection of Ralstonia solanacearum in symptomless potato tubers was achieved by efficient enrichment followed by a reliable double-antibody sandwich indirect enzyme-linked immunosorbent assay based on the specific monoclonal antibody 8B-IVIA. This monoclonal antibody reacted with 168 typical R. solanacearum strains and did not recognize 174 other pathogenic or unidentified bacteria isolated from potato. The optimized protocol included an initial enrichment step consisting of shaking the samples in modified Wilbrink broth for 72 h at 29degreesC. This step enabled specific detection by the enzyme-linked immunosorbent assay of 1 to 10 CFU of R. solanacearum per ml of initial potato extract. Analysis of 233 commercial potato lots by this method provided results that coincided with the results of conventional methods. |
| format | article |
| id | ReDivia4978 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia49782025-04-25T14:45:04Z Enrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers Caruso, Paola Gorris, María T. Cambra, Mariano Palomo, José L. Collar, Jesús López, María M. Sensitive and specific routine detection of Ralstonia solanacearum in symptomless potato tubers was achieved by efficient enrichment followed by a reliable double-antibody sandwich indirect enzyme-linked immunosorbent assay based on the specific monoclonal antibody 8B-IVIA. This monoclonal antibody reacted with 168 typical R. solanacearum strains and did not recognize 174 other pathogenic or unidentified bacteria isolated from potato. The optimized protocol included an initial enrichment step consisting of shaking the samples in modified Wilbrink broth for 72 h at 29degreesC. This step enabled specific detection by the enzyme-linked immunosorbent assay of 1 to 10 CFU of R. solanacearum per ml of initial potato extract. Analysis of 233 commercial potato lots by this method provided results that coincided with the results of conventional methods. 2017-06-01T10:11:29Z 2017-06-01T10:11:29Z 2002 JUL 2002 article Caruso, P., Gorris, M. T., Cambra, M., Palomo, J.L., Collar, J., Lopez, M.M. (2002). Enrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers. Applied and Environmental Microbiology, 68(7), 3634-3638. 0099-2240 http://hdl.handle.net/20.500.11939/4978 10.1128/AEM.68.7.3634-3638.2002 en openAccess Impreso |
| spellingShingle | Caruso, Paola Gorris, María T. Cambra, Mariano Palomo, José L. Collar, Jesús López, María M. Enrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers |
| title | Enrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers |
| title_full | Enrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers |
| title_fullStr | Enrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers |
| title_full_unstemmed | Enrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers |
| title_short | Enrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers |
| title_sort | enrichment double antibody sandwich indirect enzyme linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of ralstonia solanacearum in asymptomatic potato tubers |
| url | http://hdl.handle.net/20.500.11939/4978 |
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