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Spot real-time RT-PCR: A method for direct detection of Plum pox virus avoiding RNA extraction

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Spot real-time RT-PCR is a method for detection of Plum pox virus using conventional ELISA plant crude extracts immobilised on paper without the need of RNA extraction. The method was validated on different prunus field samples collected in winter and springtime. Seventy-nine percent of accuracy was...

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Main Authors: Capote, Nieves, Bertolini, Edson, Martínez, M. Carmen, Olmos, Antonio, Gorris, María T., Cambra, Mariano
Other Authors: Ertunc, F.
Format: conferenceObject
Language:Inglés
Published: 2017
Online Access:http://hdl.handle.net/20.500.11939/4957
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Characterization and detection of plum pox virus using monoclonal antibodies by Asensio, M., Gorris, María T., Sanz, Antonio, Camarasa, E., Perez, E., Carbonell, Emilio A., Cambra, Mariano

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Detection and typing of prunus viruses in plant tissues and in vectors by print and spot-capture PCR, heminested-PCR and PCR-ELISA by Cambra, Mariano, Olmos, Antonio, Asensio, M., Esteban, Olga, Gorris, María T., Candresse, Thierry, Boscia, D.

Published 2017
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Diagnosis and Detection of Plum Pox Virus: State-of-the-Art and Future Options by Cambra, Mariano, Bertolini, Edson, Martínez, M. Carmen, Gorris, María T., Vidal, Eduardo, Olmos, Antonio

Published 2017
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Epidemiology of Plum pox virus in Japanese plums in Spain by Cambra, Mariano, Gorris, María T., Capote, Nieves, Asensio, M., Martínez, M. Carmen, Bertolini, Edson, Collado, C., Hermoso-De-Mendoza, Alfonso, Mataix-Gato, Emilio, Lopez, A.

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European protocol for diagnosis of Erwinia amylovora by López, María M., Llop, Pablo, Gorris, María T., Penyalver, Javier, Donat, Victoria, Cambra, Mariano, Keck, M.

Published 2017
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Fire blight in Spain: Situation and monitoring by López, María M., Gorris, María T., Llop, Pablo, Cambra, Mariano, Roselló, Montserrat, Berra, D., Borruel, M., Plaza, B., Garcia, P., Palomo, José L.

Published 2017
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Isothermal amplification for detection of Plum pox virus by Olmos, Antonio, Bertolini, Edson, Cambra, Mariano

Published 2017
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Quantitative trait loci and candidate gene analysis of citrus tristeza virus-citradia interaction by Asins, María J., Bernet, Guillermo P., Ruiz, C., Cambra, Mariano, Puchades, Juan, Carbonell, Emilio A.

Published 2017
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Real-time RT-PCR for quantitative detection of Plum pox virus by Olmos, Antonio, Bertolini, Edson, Gil, Maite, Cambra, Mariano

Published 2017
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Standardization of diagnostic protocols (Diagpro) for Erwinia amylovora in the European Union by López, María M., Gorris, María T., Llop, Pablo, Penyalver, Javier, Cambra, Mariano

Published 2017
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Strategies for the control of Plum Pox Virus in apricot in Mediterranean areas by Cambra, Mariano

Published 2017
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Thirteen years of sharka disease in Valencia, Spain by Llácer, Gerardo, Cambra, Mariano

Published 2017
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