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Detection and typing of prunus viruses in plant tissues and in vectors by print and spot-capture PCR, heminested-PCR and PCR-ELISA

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A very sensitive and simple procedure has been developed for the preparation of plant and insect vector samples before PCR amplification using direct tissue printing or squashing on paper. The print-capture PCR (PC-PCR) technique was successfully used for the detection of plum pox potyvirus (PPV), a...

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Main Authors: Cambra, Mariano, Olmos, Antonio, Asensio, M., Esteban, Olga, Gorris, María T., Candresse, Thierry, Boscia, D.
Other Authors: Hadidi, Ahmed
Format: Objeto de conferencia
Language:Inglés
Published: 2017
Online Access:http://hdl.handle.net/20.500.11939/4940
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Characterization and detection of plum pox virus using monoclonal antibodies by Asensio, M., Gorris, María T., Sanz, Antonio, Camarasa, E., Perez, E., Carbonell, Emilio A., Cambra, Mariano

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Diagnosis and Detection of Plum Pox Virus: State-of-the-Art and Future Options by Cambra, Mariano, Bertolini, Edson, Martínez, M. Carmen, Gorris, María T., Vidal, Eduardo, Olmos, Antonio

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Epidemiology of Plum pox virus in Japanese plums in Spain by Cambra, Mariano, Gorris, María T., Capote, Nieves, Asensio, M., Martínez, M. Carmen, Bertolini, Edson, Collado, C., Hermoso-De-Mendoza, Alfonso, Mataix-Gato, Emilio, Lopez, A.

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European protocol for diagnosis of Erwinia amylovora by López, María M., Llop, Pablo, Gorris, María T., Penyalver, Javier, Donat, Victoria, Cambra, Mariano, Keck, M.

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Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral in... by Gil, Maite, Esteban, Olga, Martínez, M. Carmen, Garcia, Juan Antonio

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Fire blight in Spain: Situation and monitoring by López, María M., Gorris, María T., Llop, Pablo, Cambra, Mariano, Roselló, Montserrat, Berra, D., Borruel, M., Plaza, B., Garcia, P., Palomo, José L.

Published 2017
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Isothermal amplification for detection of Plum pox virus by Olmos, Antonio, Bertolini, Edson, Cambra, Mariano

Published 2017
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Quantitative trait loci and candidate gene analysis of citrus tristeza virus-citradia interaction by Asins, María J., Bernet, Guillermo P., Ruiz, C., Cambra, Mariano, Puchades, Juan, Carbonell, Emilio A.

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Real-time RT-PCR for quantitative detection of Plum pox virus by Olmos, Antonio, Bertolini, Edson, Gil, Maite, Cambra, Mariano

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Spot real-time RT-PCR: A method for direct detection of Plum pox virus avoiding RNA extraction by Capote, Nieves, Bertolini, Edson, Martínez, M. Carmen, Olmos, Antonio, Gorris, María T., Cambra, Mariano

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Standardization of diagnostic protocols (Diagpro) for Erwinia amylovora in the European Union by López, María M., Gorris, María T., Llop, Pablo, Penyalver, Javier, Cambra, Mariano

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Strategies for the control of Plum Pox Virus in apricot in Mediterranean areas by Cambra, Mariano

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Thirteen years of sharka disease in Valencia, Spain by Llácer, Gerardo, Cambra, Mariano

Published 2017
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