Citrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus.
To invade systemically host plants, viruses need to replicate in the infected cells, spread to neighbouring cells through plasmodesmata and move to distal parts of the plant via sieve tubes to start new infection foci. To monitor the infection of Nicotiana benthamiana plants by Citrus leaf blotch vi...
| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4910 |
| _version_ | 1855032195353149440 |
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| author | Agüero, Jesús Vives, María C. Velázquez, Karelia Ruiz-Ruiz, Susana Juárez, José Navarro, Luis Moreno, Pedro Guerri, José |
| author_browse | Agüero, Jesús Guerri, José Juárez, José Moreno, Pedro Navarro, Luis Ruiz-Ruiz, Susana Velázquez, Karelia Vives, María C. |
| author_facet | Agüero, Jesús Vives, María C. Velázquez, Karelia Ruiz-Ruiz, Susana Juárez, José Navarro, Luis Moreno, Pedro Guerri, José |
| author_sort | Agüero, Jesús |
| collection | ReDivia |
| description | To invade systemically host plants, viruses need to replicate in the infected cells, spread to neighbouring cells through plasmodesmata and move to distal parts of the plant via sieve tubes to start new infection foci. To monitor the infection of Nicotiana benthamiana plants by Citrus leaf blotch virus (CLBV), leaves were agroinoculated with an infectious cDNA clone of the CLBV genomic RNA expressing green fluorescent protein (GFP) under the transcriptional control of a duplicate promoter of the coat protein subgenomic RNA. Fluorescent spots first appeared in agroinfiltrated leaves 11-12 days after infiltration, indicating CLBV replication. Then, after entering the phloem vascular system, CLBV was unloaded in the upper parts of the plant and invaded all tissues, including flower organs and meristems. GFP fluorescence was not visible in citrus plants infected with CLBV-GFP. Therefore, to detect CLBV in meristematic regions, Mexican lime (Citrus aurantifolia) plants were graft inoculated with CLBV, with Citrus tristeza virus (CTV), a virus readily eliminated by shoot-tip grafting invitro, or with both simultaneously. Although CLBV was detected by hybridization and real-time reverse transcription-polymerase chain reaction (RT-PCR) in 0.2-mm shoot tips in all CLBV-inoculated plants, CTV was not detected. These results explain the difficulty in eliminating CLBV by shoot-tip grafting invitro. |
| format | article |
| id | ReDivia4910 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia49102025-04-25T14:44:53Z Citrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus. Agüero, Jesús Vives, María C. Velázquez, Karelia Ruiz-Ruiz, Susana Juárez, José Navarro, Luis Moreno, Pedro Guerri, José To invade systemically host plants, viruses need to replicate in the infected cells, spread to neighbouring cells through plasmodesmata and move to distal parts of the plant via sieve tubes to start new infection foci. To monitor the infection of Nicotiana benthamiana plants by Citrus leaf blotch virus (CLBV), leaves were agroinoculated with an infectious cDNA clone of the CLBV genomic RNA expressing green fluorescent protein (GFP) under the transcriptional control of a duplicate promoter of the coat protein subgenomic RNA. Fluorescent spots first appeared in agroinfiltrated leaves 11-12 days after infiltration, indicating CLBV replication. Then, after entering the phloem vascular system, CLBV was unloaded in the upper parts of the plant and invaded all tissues, including flower organs and meristems. GFP fluorescence was not visible in citrus plants infected with CLBV-GFP. Therefore, to detect CLBV in meristematic regions, Mexican lime (Citrus aurantifolia) plants were graft inoculated with CLBV, with Citrus tristeza virus (CTV), a virus readily eliminated by shoot-tip grafting invitro, or with both simultaneously. Although CLBV was detected by hybridization and real-time reverse transcription-polymerase chain reaction (RT-PCR) in 0.2-mm shoot tips in all CLBV-inoculated plants, CTV was not detected. These results explain the difficulty in eliminating CLBV by shoot-tip grafting invitro. 2017-06-01T10:11:19Z 2017-06-01T10:11:19Z 2013 2013-Aug article publishedVersion Aguero, Jesus, Vives, M.C., Velazquez, Karelia, Ruiz-Ruiz, S., Juarez, J., Navarro, L., Moreno, P., Guerri, J. (2013). Citrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus.. Molecular plant pathology, 14(6), 610-6. 1364-3703 http://hdl.handle.net/20.500.11939/4910 10.1111/mpp.12031 en openAccess Impreso |
| spellingShingle | Agüero, Jesús Vives, María C. Velázquez, Karelia Ruiz-Ruiz, Susana Juárez, José Navarro, Luis Moreno, Pedro Guerri, José Citrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus. |
| title | Citrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus. |
| title_full | Citrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus. |
| title_fullStr | Citrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus. |
| title_full_unstemmed | Citrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus. |
| title_short | Citrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus. |
| title_sort | citrus leaf blotch virus invades meristematic regions in nicotiana benthamiana and citrus |
| url | http://hdl.handle.net/20.500.11939/4910 |
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