Cl- homeostasis in includer and excluder citrus rootstocks: transport mechanisms and identification of candidate genes
To reveal specific Cl- transport activities in the symplastic pathway, uptake, long-distance transport and distribution of Cl- have been investigated in the citrus rootstocks Carrizo citrange (CC, Cl- includer) and Cleopatra mandarin (CM, Cl- excluder). Using an external concentration of 4.5 mm Cl-,...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4906 |
| _version_ | 1855032194618097664 |
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| author | Brumos, Javier Talón, Manuel Bouhlel, Rym Colmenero-Flores, José M. |
| author_browse | Bouhlel, Rym Brumos, Javier Colmenero-Flores, José M. Talón, Manuel |
| author_facet | Brumos, Javier Talón, Manuel Bouhlel, Rym Colmenero-Flores, José M. |
| author_sort | Brumos, Javier |
| collection | ReDivia |
| description | To reveal specific Cl- transport activities in the symplastic pathway, uptake, long-distance transport and distribution of Cl- have been investigated in the citrus rootstocks Carrizo citrange (CC, Cl- includer) and Cleopatra mandarin (CM, Cl- excluder). Using an external concentration of 4.5 mm Cl-, both species actively transported Cl- to levels that exceeded the critical requirement concentration by one and two orders of magnitude in the excluder and the includer rootstocks, respectively. Both CC and CM modulated Cl- influx according to the availability of the nutrient as uptake capacity was induced by Cl- starvation, but inhibited after Cl- resupply. Net Cl- uptake was higher in the includer CC, an observation that correlated with a lower root-to-shoot transport capacity in the excluder CM. The patterns of tissue Cl- accumulation indicated that chloride exclusion in the salt-tolerant rootstock CM was caused by a reduced net Cl- loading into the root xylem. Genes CcCCC1, CcSLAH1 and CcICln1 putatively involved in the regulation of chloride transport were isolated and their expression analysed in response to both changes in the nutritional status of Cl- and salt stress. The previously uncharacterized ICln gene exhibited a strong repression to Cl- application in the excluder rootstock, suggesting a role in regulating Cl- homeostasis in plants. |
| format | article |
| id | ReDivia4906 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia49062025-04-25T14:44:53Z Cl- homeostasis in includer and excluder citrus rootstocks: transport mechanisms and identification of candidate genes Brumos, Javier Talón, Manuel Bouhlel, Rym Colmenero-Flores, José M. To reveal specific Cl- transport activities in the symplastic pathway, uptake, long-distance transport and distribution of Cl- have been investigated in the citrus rootstocks Carrizo citrange (CC, Cl- includer) and Cleopatra mandarin (CM, Cl- excluder). Using an external concentration of 4.5 mm Cl-, both species actively transported Cl- to levels that exceeded the critical requirement concentration by one and two orders of magnitude in the excluder and the includer rootstocks, respectively. Both CC and CM modulated Cl- influx according to the availability of the nutrient as uptake capacity was induced by Cl- starvation, but inhibited after Cl- resupply. Net Cl- uptake was higher in the includer CC, an observation that correlated with a lower root-to-shoot transport capacity in the excluder CM. The patterns of tissue Cl- accumulation indicated that chloride exclusion in the salt-tolerant rootstock CM was caused by a reduced net Cl- loading into the root xylem. Genes CcCCC1, CcSLAH1 and CcICln1 putatively involved in the regulation of chloride transport were isolated and their expression analysed in response to both changes in the nutritional status of Cl- and salt stress. The previously uncharacterized ICln gene exhibited a strong repression to Cl- application in the excluder rootstock, suggesting a role in regulating Cl- homeostasis in plants. 2017-06-01T10:11:19Z 2017-06-01T10:11:19Z 2010 DEC 2010 article Brumos, J., Talón, M., Bouhlal, Rym, Colmenero-Flores, J.M. (2010). Cl- homeostasis in includer and excluder citrus rootstocks: transport mechanisms and identification of candidate genes. Plant Cell and Environment, 33(12), 2012-2027. 0140-7791 http://hdl.handle.net/20.500.11939/4906 10.1111/j.1365-3040.2010.02202.x en openAccess Impreso |
| spellingShingle | Brumos, Javier Talón, Manuel Bouhlel, Rym Colmenero-Flores, José M. Cl- homeostasis in includer and excluder citrus rootstocks: transport mechanisms and identification of candidate genes |
| title | Cl- homeostasis in includer and excluder citrus rootstocks: transport mechanisms and identification of candidate genes |
| title_full | Cl- homeostasis in includer and excluder citrus rootstocks: transport mechanisms and identification of candidate genes |
| title_fullStr | Cl- homeostasis in includer and excluder citrus rootstocks: transport mechanisms and identification of candidate genes |
| title_full_unstemmed | Cl- homeostasis in includer and excluder citrus rootstocks: transport mechanisms and identification of candidate genes |
| title_short | Cl- homeostasis in includer and excluder citrus rootstocks: transport mechanisms and identification of candidate genes |
| title_sort | cl homeostasis in includer and excluder citrus rootstocks transport mechanisms and identification of candidate genes |
| url | http://hdl.handle.net/20.500.11939/4906 |
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