Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus
A monoclonal antibody to an Albanian isolate of plum pox potyvirus (PPV) was obtained (MAbAL), that specifically recognized strain M of this virus. The specificity of MAbAL, assessed by comparative ELISA on 130 PPV isolates of different geographical origin, 22 of which were also tested by comparativ...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4894 |
| _version_ | 1855491831399186432 |
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| author | Boscia, D. Zeramdini, H. Cambra, Mariano Potere, O. Gorris, María T. Myrta, A. DiTerlizzi, B. Savino, V. |
| author_browse | Boscia, D. Cambra, Mariano DiTerlizzi, B. Gorris, María T. Myrta, A. Potere, O. Savino, V. Zeramdini, H. |
| author_facet | Boscia, D. Zeramdini, H. Cambra, Mariano Potere, O. Gorris, María T. Myrta, A. DiTerlizzi, B. Savino, V. |
| author_sort | Boscia, D. |
| collection | ReDivia |
| description | A monoclonal antibody to an Albanian isolate of plum pox potyvirus (PPV) was obtained (MAbAL), that specifically recognized strain M of this virus. The specificity of MAbAL, assessed by comparative ELISA on 130 PPV isolates of different geographical origin, 22 of which were also tested by comparative IC-PCR, gave consistent and highly reproducible results. MAbAL seems to be elicited by a stable surface determinant that makes it particularly suitable for successful use under a wide range of conditions. MAbAL is an useful addition to the panel of PPV-specific MAbs available to date. |
| format | Artículo |
| id | ReDivia4894 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia48942025-04-25T14:44:52Z Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus Boscia, D. Zeramdini, H. Cambra, Mariano Potere, O. Gorris, María T. Myrta, A. DiTerlizzi, B. Savino, V. A monoclonal antibody to an Albanian isolate of plum pox potyvirus (PPV) was obtained (MAbAL), that specifically recognized strain M of this virus. The specificity of MAbAL, assessed by comparative ELISA on 130 PPV isolates of different geographical origin, 22 of which were also tested by comparative IC-PCR, gave consistent and highly reproducible results. MAbAL seems to be elicited by a stable surface determinant that makes it particularly suitable for successful use under a wide range of conditions. MAbAL is an useful addition to the panel of PPV-specific MAbs available to date. 2017-06-01T10:11:17Z 2017-06-01T10:11:17Z 1997 JUL 1997 article Boscia, D., Zeramdini, H., Cambra, M., Potere, O., Gorris, M. T., Myrta, A., DiTerlizzi, B., Savino, V. (1997). Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus. European Journal of Plant Pathology, 103(5), 477-480. 0929-1873 http://hdl.handle.net/20.500.11939/4894 10.1023/A:1008674618635 en openAccess Impreso |
| spellingShingle | Boscia, D. Zeramdini, H. Cambra, Mariano Potere, O. Gorris, María T. Myrta, A. DiTerlizzi, B. Savino, V. Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus |
| title | Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus |
| title_full | Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus |
| title_fullStr | Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus |
| title_full_unstemmed | Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus |
| title_short | Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus |
| title_sort | production and characterization of a monoclonal antibody specific to the m serotype of plum pox potyvirus |
| url | http://hdl.handle.net/20.500.11939/4894 |
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