Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants
Virus induced gene silencing (VIGS) is an effective technology for gene function analysis in plants. We assessed the VIGS effectiveness in Nicotiana benthamiana and citrus plants of different Citrus leaf blotch virus (CLBV)-based vectors, using insets of the phytoene desaturase (pds) gene. While in...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4888 |
| _version_ | 1855491830399893504 |
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| author | Agüero, Jesús Vives, María C. Velázquez, Karelia Pina, José A. Navarro, Luis Moreno, Pedro Guerri, José |
| author_browse | Agüero, Jesús Guerri, José Moreno, Pedro Navarro, Luis Pina, José A. Velázquez, Karelia Vives, María C. |
| author_facet | Agüero, Jesús Vives, María C. Velázquez, Karelia Pina, José A. Navarro, Luis Moreno, Pedro Guerri, José |
| author_sort | Agüero, Jesús |
| collection | ReDivia |
| description | Virus induced gene silencing (VIGS) is an effective technology for gene function analysis in plants. We assessed the VIGS effectiveness in Nicotiana benthamiana and citrus plants of different Citrus leaf blotch virus (CLBV)-based vectors, using insets of the phytoene desaturase (pds) gene. While in N. benthamiana the silencing phenotype was induced only by the construct carrying a 58-nt pds hairpin, in citrus plants all the constructs induced the silencing phenotype. Differences in the generation of secondary small interfering RNAs in both species are believed to be responsible for differential host-species effects. The ability of CLBV-based vectors to silence different endogenous citrus genes was further confirmed. Since CLBV-based vectors are known to be stable and induce VIGS in successive flushes for several months, these vectors provide an important genomic tool and it is expected that they will be useful to analyze gene function by reverse genetics in the long-lived citrus plants. |
| format | Artículo |
| id | ReDivia4888 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia48882025-04-25T14:44:51Z Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants Agüero, Jesús Vives, María C. Velázquez, Karelia Pina, José A. Navarro, Luis Moreno, Pedro Guerri, José Virus induced gene silencing (VIGS) is an effective technology for gene function analysis in plants. We assessed the VIGS effectiveness in Nicotiana benthamiana and citrus plants of different Citrus leaf blotch virus (CLBV)-based vectors, using insets of the phytoene desaturase (pds) gene. While in N. benthamiana the silencing phenotype was induced only by the construct carrying a 58-nt pds hairpin, in citrus plants all the constructs induced the silencing phenotype. Differences in the generation of secondary small interfering RNAs in both species are believed to be responsible for differential host-species effects. The ability of CLBV-based vectors to silence different endogenous citrus genes was further confirmed. Since CLBV-based vectors are known to be stable and induce VIGS in successive flushes for several months, these vectors provide an important genomic tool and it is expected that they will be useful to analyze gene function by reverse genetics in the long-lived citrus plants. 2017-06-01T10:11:16Z 2017-06-01T10:11:16Z 2014 JUL 2014 article Aguero, Jesus, del C. Vives, M., Velazquez, Karelia, A. Pina, J., Navarro, L., Moreno, P., Guerri, J. (2014). Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants. Virology, 460, 154-164. 0042-6822 http://hdl.handle.net/20.500.11939/4888 10.1016/j.virol.2014.04.017 en openAccess Impreso |
| spellingShingle | Agüero, Jesús Vives, María C. Velázquez, Karelia Pina, José A. Navarro, Luis Moreno, Pedro Guerri, José Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants |
| title | Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants |
| title_full | Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants |
| title_fullStr | Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants |
| title_full_unstemmed | Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants |
| title_short | Effectiveness of gene silencing induced by viral vectors based on Citrus leaf blotch virus is different in Nicotiana benthamiana and citrus plants |
| title_sort | effectiveness of gene silencing induced by viral vectors based on citrus leaf blotch virus is different in nicotiana benthamiana and citrus plants |
| url | http://hdl.handle.net/20.500.11939/4888 |
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