Response of Boar Sperm to the Treatment with Cholesterol-Loaded Cyclodextrins Added Prior to Cryopreservation
Cryopreserved boar sperm is not used extensively for artificial insemination, owing to the poor fertility rates of the sperm after freezing and thawing. The sperm membrane is damaged as the cells are cooled from body temperature to 5 degrees C (cold shock), as well as during the freezethaw process....
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4851 |
| _version_ | 1855491824970366976 |
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| author | Blanch, Eva Tomás, Cristina Graham, James K. Mocé, Eva |
| author_browse | Blanch, Eva Graham, James K. Mocé, Eva Tomás, Cristina |
| author_facet | Blanch, Eva Tomás, Cristina Graham, James K. Mocé, Eva |
| author_sort | Blanch, Eva |
| collection | ReDivia |
| description | Cryopreserved boar sperm is not used extensively for artificial insemination, owing to the poor fertility rates of the sperm after freezing and thawing. The sperm membrane is damaged as the cells are cooled from body temperature to 5 degrees C (cold shock), as well as during the freezethaw process. Increasing the cholesterol content of boar sperm membranes could help them survive cryopreservation, similar to sperm from other species that are cold shock sensitive. The aim of this study was to determine the optimal cholesterol-loaded cyclodextrin (CLC) concentration to use for boar sperm cryopreservation, and the influence of CLCs on the cryosurvival of sperm from boars classified as good or poor freezers. Treating boar sperm with 1 mg of CLC/120 x 10(6)sperm slightly improved (p < 0.05) the percentage of viable sperm after freezingthawing. On the other hand, sperm, from both good and poor freezers, responded similarly to CLC treatment. Nevertheless, additional studies will be needed to study the effect of this treatment on other parameters of sperm quality. |
| format | Artículo |
| id | ReDivia4851 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia48512025-04-25T14:44:47Z Response of Boar Sperm to the Treatment with Cholesterol-Loaded Cyclodextrins Added Prior to Cryopreservation Blanch, Eva Tomás, Cristina Graham, James K. Mocé, Eva Cryopreserved boar sperm is not used extensively for artificial insemination, owing to the poor fertility rates of the sperm after freezing and thawing. The sperm membrane is damaged as the cells are cooled from body temperature to 5 degrees C (cold shock), as well as during the freezethaw process. Increasing the cholesterol content of boar sperm membranes could help them survive cryopreservation, similar to sperm from other species that are cold shock sensitive. The aim of this study was to determine the optimal cholesterol-loaded cyclodextrin (CLC) concentration to use for boar sperm cryopreservation, and the influence of CLCs on the cryosurvival of sperm from boars classified as good or poor freezers. Treating boar sperm with 1 mg of CLC/120 x 10(6)sperm slightly improved (p < 0.05) the percentage of viable sperm after freezingthawing. On the other hand, sperm, from both good and poor freezers, responded similarly to CLC treatment. Nevertheless, additional studies will be needed to study the effect of this treatment on other parameters of sperm quality. 2017-06-01T10:11:10Z 2017-06-01T10:11:10Z 2012 DEC 2012 article Blanch, E., Tomas, C., Graham, J.K., Moce, E. (2012). Response of Boar Sperm to the Treatment with CholE.ol-Loaded Cyclodextrins Added Prior to Cryopreservation. Reproduction in Domestic Animals, 47(6), 959-964. 0936-6768 http://hdl.handle.net/20.500.11939/4851 10.1111/j.1439-0531.2012.01999.x en openAccess Impreso |
| spellingShingle | Blanch, Eva Tomás, Cristina Graham, James K. Mocé, Eva Response of Boar Sperm to the Treatment with Cholesterol-Loaded Cyclodextrins Added Prior to Cryopreservation |
| title | Response of Boar Sperm to the Treatment with Cholesterol-Loaded Cyclodextrins Added Prior to Cryopreservation |
| title_full | Response of Boar Sperm to the Treatment with Cholesterol-Loaded Cyclodextrins Added Prior to Cryopreservation |
| title_fullStr | Response of Boar Sperm to the Treatment with Cholesterol-Loaded Cyclodextrins Added Prior to Cryopreservation |
| title_full_unstemmed | Response of Boar Sperm to the Treatment with Cholesterol-Loaded Cyclodextrins Added Prior to Cryopreservation |
| title_short | Response of Boar Sperm to the Treatment with Cholesterol-Loaded Cyclodextrins Added Prior to Cryopreservation |
| title_sort | response of boar sperm to the treatment with cholesterol loaded cyclodextrins added prior to cryopreservation |
| url | http://hdl.handle.net/20.500.11939/4851 |
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