Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms
Sampling techniques to detect airborne Salmonella species (spp.) in two pilot scale broiler houses were compared. Broilers were inoculated at seven days of age with a marked strain of Salmonella enteritidis. The rearing cycle lasted 42 days during the summer. Airborne Salmonella spp. were sampled we...
| Main Authors: | , , , , , |
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| Format: | article |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/4844 |
| _version_ | 1855032186126729216 |
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| author | Adell, Elisa Moset, Verónica Zhao, Yang Jiménez-Belenguer, Ana Cerisuelo, Alba Cambra-López, María |
| author_browse | Adell, Elisa Cambra-López, María Cerisuelo, Alba Jiménez-Belenguer, Ana Moset, Verónica Zhao, Yang |
| author_facet | Adell, Elisa Moset, Verónica Zhao, Yang Jiménez-Belenguer, Ana Cerisuelo, Alba Cambra-López, María |
| author_sort | Adell, Elisa |
| collection | ReDivia |
| description | Sampling techniques to detect airborne Salmonella species (spp.) in two pilot scale broiler houses were compared. Broilers were inoculated at seven days of age with a marked strain of Salmonella enteritidis. The rearing cycle lasted 42 days during the summer. Airborne Salmonella spp. were sampled weekly using impaction, gravitational settling, and impingement techniques. Additionally,Salmonella spp. were sampled on feeders, drinkers, walls, and in the litter. Environmental conditions (temperature, relative humidity, and airborne particulate matter (PM) concentration) were monitored during the rearing cycle. The presence of Salmonella spp. was determined by culture-dependent and molecular methods. No cultivable Salmonella spp. were recovered from the poultry houses' surfaces, the litter, or the air before inoculation. After inoculation, cultivable Salmonella spp. were recovered from the surfaces and in the litter. Airborne cultivable Salmonella spp. were detected using impaction and gravitational settling one or two weeks after the detection of Salmonella spp. in the litter. No cultivable Salmonella spp. were recovered using impingement based on culture-dependent techniques. At low airborne concentrations, the use of impingement for the quantification or detection of cultivable airborne Salmonella spp. is not recommended. In these cases, a combination of culture-dependent and culture-independent methods is recommended. These data are valuable to improve current measures to control the transmission of pathogens in livestock environments and for optimising the sampling and detection of airborne Salmonella spp. in practical conditions. |
| format | article |
| id | ReDivia4844 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia48442025-04-25T14:44:46Z Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms Adell, Elisa Moset, Verónica Zhao, Yang Jiménez-Belenguer, Ana Cerisuelo, Alba Cambra-López, María Sampling techniques to detect airborne Salmonella species (spp.) in two pilot scale broiler houses were compared. Broilers were inoculated at seven days of age with a marked strain of Salmonella enteritidis. The rearing cycle lasted 42 days during the summer. Airborne Salmonella spp. were sampled weekly using impaction, gravitational settling, and impingement techniques. Additionally,Salmonella spp. were sampled on feeders, drinkers, walls, and in the litter. Environmental conditions (temperature, relative humidity, and airborne particulate matter (PM) concentration) were monitored during the rearing cycle. The presence of Salmonella spp. was determined by culture-dependent and molecular methods. No cultivable Salmonella spp. were recovered from the poultry houses' surfaces, the litter, or the air before inoculation. After inoculation, cultivable Salmonella spp. were recovered from the surfaces and in the litter. Airborne cultivable Salmonella spp. were detected using impaction and gravitational settling one or two weeks after the detection of Salmonella spp. in the litter. No cultivable Salmonella spp. were recovered using impingement based on culture-dependent techniques. At low airborne concentrations, the use of impingement for the quantification or detection of cultivable airborne Salmonella spp. is not recommended. In these cases, a combination of culture-dependent and culture-independent methods is recommended. These data are valuable to improve current measures to control the transmission of pathogens in livestock environments and for optimising the sampling and detection of airborne Salmonella spp. in practical conditions. 2017-06-01T10:11:09Z 2017-06-01T10:11:09Z 2014 2014 article publishedVersion Adell, Elisa, Moset, Veronica, Zhao, Yang, Jimenez-B.guer, Ana, Cerisuelo, Alba, Cambra-Lopez, M. (2014). Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms. Annals of Agricultural and Environmental Medicine, 21(1), 15-24. 1232-1966 http://hdl.handle.net/20.500.11939/4844 en openAccess Impreso |
| spellingShingle | Adell, Elisa Moset, Verónica Zhao, Yang Jiménez-Belenguer, Ana Cerisuelo, Alba Cambra-López, María Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms |
| title | Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms |
| title_full | Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms |
| title_fullStr | Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms |
| title_full_unstemmed | Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms |
| title_short | Comparative performance of three sampling techniques to detect airborne Salmonella species in poultry farms |
| title_sort | comparative performance of three sampling techniques to detect airborne salmonella species in poultry farms |
| url | http://hdl.handle.net/20.500.11939/4844 |
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