Quantitative detection of Citrus tristeza virus in plant tissues and single aphids by real-time RT-PCR
TaqMan real-time reverse transcriptase (RT)-polymerase chain reaction (PCR) using purified RNA targets or coupled to tissue-print and squash procedures was developed to detect and quantify Citrus tristeza virus (CTV) RNA-targets in plant tissues and in single aphids. With this method all CTV isolate...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/4841 |
Ejemplares similares: Quantitative detection of Citrus tristeza virus in plant tissues and single aphids by real-time RT-PCR
- Real-time RT-PCR for quantitative detection of Plum pox virus
- Direct sample preparation methods for the detection of Plum pox virus by real-time RT-PCR
- Nested rt-PCR in a single closed tube.
- Molecular methods for detection and quantitation of virus in aphids
- Real-time assay for quantitative detection of non-persistently transmitted Plum pox virus RNA targets in single aphids
- Spot real-time RT-PCR: A method for direct detection of Plum pox virus avoiding RNA extraction