Highly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube

A nested-polymerase chain reaction (PCR) has been set up to be performed in a single closed tube for the detection of Pseudomonas savastanoi pv. savastanoi. Nested-PCR coupled with dot-blot hybridization was able to detect up to one cell of the target per ml of olive extract, showing the greatest se...

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Main Authors: Bertolini, Edson, Penyalver, Ramón, Garcia, Amparo, Olmos, Antonio, Quesada, Jose M., Cambra, Mariano, López, María M.
Format: Artículo
Language:Inglés
Published: 2017
Online Access:http://hdl.handle.net/20.500.11939/4837
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author Bertolini, Edson
Penyalver, Ramón
Garcia, Amparo
Olmos, Antonio
Quesada, Jose M.
Cambra, Mariano
López, María M.
author_browse Bertolini, Edson
Cambra, Mariano
Garcia, Amparo
López, María M.
Olmos, Antonio
Penyalver, Ramón
Quesada, Jose M.
author_facet Bertolini, Edson
Penyalver, Ramón
Garcia, Amparo
Olmos, Antonio
Quesada, Jose M.
Cambra, Mariano
López, María M.
author_sort Bertolini, Edson
collection ReDivia
description A nested-polymerase chain reaction (PCR) has been set up to be performed in a single closed tube for the detection of Pseudomonas savastanoi pv. savastanoi. Nested-PCR coupled with dot-blot hybridization was able to detect up to one cell of the target per ml of olive extract, showing the greatest sensitivity compared with all previously reported detection assays. Validation of the developed procedure for diagnosis and epidemiological purposes was achieved by testing ca. 240 asymptomatic plant samples from olive trees. When performing the other previously reported techniques (bacterial isolation and single PCR), P. savastanoi was detected in 50 of the analyzed samples, while with the new developed nested-PCR assay, the bacterium was detected in 82 samples.
format Artículo
id ReDivia4837
institution Instituto Valenciano de Investigaciones Agrarias (IVIA)
language Inglés
publishDate 2017
publishDateRange 2017
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spelling ReDivia48372025-04-25T14:44:45Z Highly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube Bertolini, Edson Penyalver, Ramón Garcia, Amparo Olmos, Antonio Quesada, Jose M. Cambra, Mariano López, María M. A nested-polymerase chain reaction (PCR) has been set up to be performed in a single closed tube for the detection of Pseudomonas savastanoi pv. savastanoi. Nested-PCR coupled with dot-blot hybridization was able to detect up to one cell of the target per ml of olive extract, showing the greatest sensitivity compared with all previously reported detection assays. Validation of the developed procedure for diagnosis and epidemiological purposes was achieved by testing ca. 240 asymptomatic plant samples from olive trees. When performing the other previously reported techniques (bacterial isolation and single PCR), P. savastanoi was detected in 50 of the analyzed samples, while with the new developed nested-PCR assay, the bacterium was detected in 82 samples. 2017-06-01T10:11:08Z 2017-06-01T10:11:08Z 2003 FEB 2003 article Bertolini, E., Penyalver, R., Garcia, A., Olmos, A., Quesada, J.M., Cambra, M., Lopez, M.M. (2003). Highly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube. Journal of microbiological methods, 52(2), 261-266. 0167-7012 http://hdl.handle.net/20.500.11939/4837 10.1016/S0167-7012(02)00163-X en openAccess Impreso
spellingShingle Bertolini, Edson
Penyalver, Ramón
Garcia, Amparo
Olmos, Antonio
Quesada, Jose M.
Cambra, Mariano
López, María M.
Highly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube
title Highly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube
title_full Highly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube
title_fullStr Highly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube
title_full_unstemmed Highly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube
title_short Highly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube
title_sort highly sensitive detection of pseudomonas savastanoi pv savastanoi in asymptomatic olive plants by nested pcr in a single closed tube
url http://hdl.handle.net/20.500.11939/4837
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