Molecular discrimination of lemon cultivars
Some lemon [Citrus Union (L.) Burm. f.] cultivars present compatibility problems with commonly used citrus rootstocks. Therefore, assessing trueness-to-type of lemon mother trees is needed by growers. Morphological differentiation of lemon cultivars is not precise because they present high phenotypi...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4828 https://journals.ashs.org/hortsci/view/journals/hortsci/39/1/article-p165.xml |
| _version_ | 1855032183700324352 |
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| author | Bernet, Guillermo P. Mestre, P. F. Pina, José A. Asins, María J. |
| author_browse | Asins, María J. Bernet, Guillermo P. Mestre, P. F. Pina, José A. |
| author_facet | Bernet, Guillermo P. Mestre, P. F. Pina, José A. Asins, María J. |
| author_sort | Bernet, Guillermo P. |
| collection | ReDivia |
| description | Some lemon [Citrus Union (L.) Burm. f.] cultivars present compatibility problems with commonly used citrus rootstocks. Therefore, assessing trueness-to-type of lemon mother trees is needed by growers. Morphological differentiation of lemon cultivars is not precise because they present high phenotypic plasticity. The objective of this paper is to contrast the discriminatory ability of several molecular marker systems for lemon. Three marker types were used: randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeats (ISSR) and inter-retrotransposon amplified polymorphism (IRAP). The molecular variability found in C. limon is larger than that reported for C. clementina, another vegetatively propagated citrus species. This difference in variability content might be explained by differences in the distribution and age of both cultures. Similar to clementines, polymerase chain reaction (PCR) markers using primers anchored in copya-like retrotransposons resulted in a higher rate of polymorphisms (36.4%) than did primers of random sequence (27.3%) or those anchored at simple sequence repeats (0%), indicating a higher molecular variability at the locations where these retrotransposons inserted. Primers anchored in gypsy-like retroelements did not yield polymorphisms. Lemon cultivars from important groups such as 'Eureka', 'Fino', and 'Verna' could be distinguished using all polymorphisms. |
| format | article |
| id | ReDivia4828 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia48282025-04-25T14:44:44Z Molecular discrimination of lemon cultivars Bernet, Guillermo P. Mestre, P. F. Pina, José A. Asins, María J. Some lemon [Citrus Union (L.) Burm. f.] cultivars present compatibility problems with commonly used citrus rootstocks. Therefore, assessing trueness-to-type of lemon mother trees is needed by growers. Morphological differentiation of lemon cultivars is not precise because they present high phenotypic plasticity. The objective of this paper is to contrast the discriminatory ability of several molecular marker systems for lemon. Three marker types were used: randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeats (ISSR) and inter-retrotransposon amplified polymorphism (IRAP). The molecular variability found in C. limon is larger than that reported for C. clementina, another vegetatively propagated citrus species. This difference in variability content might be explained by differences in the distribution and age of both cultures. Similar to clementines, polymerase chain reaction (PCR) markers using primers anchored in copya-like retrotransposons resulted in a higher rate of polymorphisms (36.4%) than did primers of random sequence (27.3%) or those anchored at simple sequence repeats (0%), indicating a higher molecular variability at the locations where these retrotransposons inserted. Primers anchored in gypsy-like retroelements did not yield polymorphisms. Lemon cultivars from important groups such as 'Eureka', 'Fino', and 'Verna' could be distinguished using all polymorphisms. 2017-06-01T10:11:07Z 2017-06-01T10:11:07Z 2004 FEB 2004 article publishedVersion Bernet, G. P., Mestre, P.F., Pina, J.A., Asins, M.J. (2004). Molecular discrimination of lemon cultivars. HortScience, 39(1), 165-169. 0018-5345 http://hdl.handle.net/20.500.11939/4828 10.21273/HORTSCI.39.1.165 https://journals.ashs.org/hortsci/view/journals/hortsci/39/1/article-p165.xml en openAccess Impreso |
| spellingShingle | Bernet, Guillermo P. Mestre, P. F. Pina, José A. Asins, María J. Molecular discrimination of lemon cultivars |
| title | Molecular discrimination of lemon cultivars |
| title_full | Molecular discrimination of lemon cultivars |
| title_fullStr | Molecular discrimination of lemon cultivars |
| title_full_unstemmed | Molecular discrimination of lemon cultivars |
| title_short | Molecular discrimination of lemon cultivars |
| title_sort | molecular discrimination of lemon cultivars |
| url | http://hdl.handle.net/20.500.11939/4828 https://journals.ashs.org/hortsci/view/journals/hortsci/39/1/article-p165.xml |
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