Hormonal Profile in Ovaries of Mandarin Varieties with Differing Reproductive Behaviour
The endogenous levels of 13 gibberellins (GAs), three cytokinins (CKs), abscisic acid (ABA), indole-3-acetic acid (IAA) and jasmonic acid (JA) were analyzed in naturally pollinated ovaries of three mandarin cultivars selected for their different capacity to produce seeds and their differing partheno...
| Main Authors: | , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
Springer US
2017
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.11939/4824 |
| _version_ | 1855491820292669440 |
|---|---|
| author | Bermejo, Almudena Primo-Millo, Eduardo Agustí, Manuel Mesejo, Carlos Reig, Carmina Iglesias, Domingo J. |
| author_browse | Agustí, Manuel Bermejo, Almudena Iglesias, Domingo J. Mesejo, Carlos Primo-Millo, Eduardo Reig, Carmina |
| author_facet | Bermejo, Almudena Primo-Millo, Eduardo Agustí, Manuel Mesejo, Carlos Reig, Carmina Iglesias, Domingo J. |
| author_sort | Bermejo, Almudena |
| collection | ReDivia |
| description | The endogenous levels of 13 gibberellins (GAs), three cytokinins (CKs), abscisic acid (ABA), indole-3-acetic acid (IAA) and jasmonic acid (JA) were analyzed in naturally pollinated ovaries of three mandarin cultivars selected for their different capacity to produce seeds and their differing parthenocarpic ability. The varieties compared were Murcott (pollen self-compatible, highly seeded), Moncada (self-incompatible, moderately seeded), and Moncalina (pollen sterile, seedless), obtained from Moncada by bud γ-irradiation. As expected, the 13-hydroxylation pathway was predominant in ovaries and our results further indicate that cultivar differences exist in GA metabolism. The active gibberellin GA<SUB>1</SUB> levels in ovaries seems to be related with presence of fertilized ovules and, therefore, with the ability to produce the seeds of a variety. Sterility gamma irradiation arrested the biosynthesis of GA<SUB>1</SUB> and its precursor GA<SUB>19</SUB> in Moncalina ovaries if compared to Moncada. The productive efficiency of the studied cultivars also indicated that fruit set depends strongly on the GA<SUB>1</SUB> level achieved by ovaries, which is also closely related with carbohydrate content. The study of the expression of gibberellin-oxidase genes showed that the pollination/fecundation process enhances <I>GA20ox2</I> and <I>GA3ox1</I> activities in naturally pollinated Murcott and Moncada ovaries compared with unpollinated Murcott and Moncalina, respectively. <I>GA2ox1</I> expression was lower in the ovaries of the highly seeded cultivar Murcott than in those of Moncada or Moncalina. Unpollinated Murcott ovaries contained much lower levels of GA<SUB>1</SUB> and IAA than the naturally pollinated ovaries of this cultivar. Conversely, unpollinated ovaries, which exhibited 100 % abscission, had more ABA and JA contents. Cytokinin activity seemed constitutive and independent of pollination/fecundation. However, <I>trans</I>-zeatin (<I>t</I>-Z) and 2-isopentenyl adenine (2-IP) concentrations were higher in Murcott ovaries than in Moncada/Moncalina. |
| format | Artículo |
| id | ReDivia4824 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Springer US |
| publisherStr | Springer US |
| record_format | dspace |
| spelling | ReDivia48242025-04-25T14:44:43Z Hormonal Profile in Ovaries of Mandarin Varieties with Differing Reproductive Behaviour Bermejo, Almudena Primo-Millo, Eduardo Agustí, Manuel Mesejo, Carlos Reig, Carmina Iglesias, Domingo J. Carbohydrates Citrus ovaries Cytokinins Gibberellins GA-oxidase genes Indole-3-acetic acid The endogenous levels of 13 gibberellins (GAs), three cytokinins (CKs), abscisic acid (ABA), indole-3-acetic acid (IAA) and jasmonic acid (JA) were analyzed in naturally pollinated ovaries of three mandarin cultivars selected for their different capacity to produce seeds and their differing parthenocarpic ability. The varieties compared were Murcott (pollen self-compatible, highly seeded), Moncada (self-incompatible, moderately seeded), and Moncalina (pollen sterile, seedless), obtained from Moncada by bud γ-irradiation. As expected, the 13-hydroxylation pathway was predominant in ovaries and our results further indicate that cultivar differences exist in GA metabolism. The active gibberellin GA<SUB>1</SUB> levels in ovaries seems to be related with presence of fertilized ovules and, therefore, with the ability to produce the seeds of a variety. Sterility gamma irradiation arrested the biosynthesis of GA<SUB>1</SUB> and its precursor GA<SUB>19</SUB> in Moncalina ovaries if compared to Moncada. The productive efficiency of the studied cultivars also indicated that fruit set depends strongly on the GA<SUB>1</SUB> level achieved by ovaries, which is also closely related with carbohydrate content. The study of the expression of gibberellin-oxidase genes showed that the pollination/fecundation process enhances <I>GA20ox2</I> and <I>GA3ox1</I> activities in naturally pollinated Murcott and Moncada ovaries compared with unpollinated Murcott and Moncalina, respectively. <I>GA2ox1</I> expression was lower in the ovaries of the highly seeded cultivar Murcott than in those of Moncada or Moncalina. Unpollinated Murcott ovaries contained much lower levels of GA<SUB>1</SUB> and IAA than the naturally pollinated ovaries of this cultivar. Conversely, unpollinated ovaries, which exhibited 100 % abscission, had more ABA and JA contents. Cytokinin activity seemed constitutive and independent of pollination/fecundation. However, <I>trans</I>-zeatin (<I>t</I>-Z) and 2-isopentenyl adenine (2-IP) concentrations were higher in Murcott ovaries than in Moncada/Moncalina. 2017-06-01T10:11:06Z 2017-06-01T10:11:06Z 2015 03/11 article Bermejo, Almudena, Primo-Millo, E., Agustí, M., Mesejo, C., Reig, Carmina, Iglesias, DomingoJ (2015). Hormonal Profile in Ovaries of Mandarin Varieties with Differing Reproductive Behaviour. Journal of plant growth regulation, , 1-11. 0721-7595 http://hdl.handle.net/20.500.11939/4824 10.1007/s00344-015-9492-y en openAccess Springer US Impreso |
| spellingShingle | Carbohydrates Citrus ovaries Cytokinins Gibberellins GA-oxidase genes Indole-3-acetic acid Bermejo, Almudena Primo-Millo, Eduardo Agustí, Manuel Mesejo, Carlos Reig, Carmina Iglesias, Domingo J. Hormonal Profile in Ovaries of Mandarin Varieties with Differing Reproductive Behaviour |
| title | Hormonal Profile in Ovaries of Mandarin Varieties with Differing Reproductive Behaviour |
| title_full | Hormonal Profile in Ovaries of Mandarin Varieties with Differing Reproductive Behaviour |
| title_fullStr | Hormonal Profile in Ovaries of Mandarin Varieties with Differing Reproductive Behaviour |
| title_full_unstemmed | Hormonal Profile in Ovaries of Mandarin Varieties with Differing Reproductive Behaviour |
| title_short | Hormonal Profile in Ovaries of Mandarin Varieties with Differing Reproductive Behaviour |
| title_sort | hormonal profile in ovaries of mandarin varieties with differing reproductive behaviour |
| topic | Carbohydrates Citrus ovaries Cytokinins Gibberellins GA-oxidase genes Indole-3-acetic acid |
| url | http://hdl.handle.net/20.500.11939/4824 |
| work_keys_str_mv | AT bermejoalmudena hormonalprofileinovariesofmandarinvarietieswithdifferingreproductivebehaviour AT primomilloeduardo hormonalprofileinovariesofmandarinvarietieswithdifferingreproductivebehaviour AT agustimanuel hormonalprofileinovariesofmandarinvarietieswithdifferingreproductivebehaviour AT mesejocarlos hormonalprofileinovariesofmandarinvarietieswithdifferingreproductivebehaviour AT reigcarmina hormonalprofileinovariesofmandarinvarietieswithdifferingreproductivebehaviour AT iglesiasdomingoj hormonalprofileinovariesofmandarinvarietieswithdifferingreproductivebehaviour |