Non-additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between C. reticulata and C. limon: the case of pulp carotenoid biosynthesis pathway
Allopolyploidy is known to induce novel patterns of gene expression and often gives rise to new phenotypes. Here we report on the first attempt to relate phenotypic inheritance in an allotetraploid somatic hybrid with gene expression. Carotenoid compounds in the fruit pulp of the two parental specie...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/4791 https://link.springer.com/article/10.1007%2Fs00299-009-0768-1 |
| _version_ | 1855032176984195072 |
|---|---|
| author | Bassene, Jean Baptiste Froelicher, Yann Dhuique-Mayer, Claudie Mouhaya, Waffa Ferrer, Rosa M. Ancillo, Gema Morillon, Raphael Navarro, Luis Ollitrault, Patrick |
| author_browse | Ancillo, Gema Bassene, Jean Baptiste Dhuique-Mayer, Claudie Ferrer, Rosa M. Froelicher, Yann Morillon, Raphael Mouhaya, Waffa Navarro, Luis Ollitrault, Patrick |
| author_facet | Bassene, Jean Baptiste Froelicher, Yann Dhuique-Mayer, Claudie Mouhaya, Waffa Ferrer, Rosa M. Ancillo, Gema Morillon, Raphael Navarro, Luis Ollitrault, Patrick |
| author_sort | Bassene, Jean Baptiste |
| collection | ReDivia |
| description | Allopolyploidy is known to induce novel patterns of gene expression and often gives rise to new phenotypes. Here we report on the first attempt to relate phenotypic inheritance in an allotetraploid somatic hybrid with gene expression. Carotenoid compounds in the fruit pulp of the two parental species and the hybrid were evaluated quantitatively by HPLC. Only very low levels of beta-carotene and beta-cryptoxanthin were observed in Citrus limon, while beta-cryptoxanthin was a major component of C. reticulata, which also displayed high levels of phytoene, phytofluene, beta-carotene, lutein, zeaxantin and violaxanthin. Total carotenoid content in mandarin juice sacs was 60 times greater than that in lemon. The allotetraploid hybrid produced all the same compounds as mandarin but at very low levels. Transgressive concentration of abscisic acid (ABA) was observed in the somatic hybrid. Real-time RT-PCR of total RNA from juice sacs was used to study expression of seven genes (CitDxs, CitPsy, CitPds, CitZds, CitLcy-b, CitChx-b, and CitZep) of the carotenoid biosynthetic pathway and two genes (CitNced1 and CitNced2) involved in abscisic acid synthesis from carotenoid. Gene expression was significantly higher for mandarin than lemon for seven of the nine genes analyzed. Lemon under expression was partially dominant in the somatic hybrid for three upstream steps of the biosynthetic pathway, particularly for CitDxs. Transgressive over expression was observed for the two CitNced genes. A limitation of the upstream steps of the pathway and a downstream higher consumption of carotenoids may explain the phenotype of the somatic hybrid. |
| format | article |
| id | ReDivia4791 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia47912025-04-25T14:44:26Z Non-additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between C. reticulata and C. limon: the case of pulp carotenoid biosynthesis pathway Bassene, Jean Baptiste Froelicher, Yann Dhuique-Mayer, Claudie Mouhaya, Waffa Ferrer, Rosa M. Ancillo, Gema Morillon, Raphael Navarro, Luis Ollitrault, Patrick Allopolyploidy is known to induce novel patterns of gene expression and often gives rise to new phenotypes. Here we report on the first attempt to relate phenotypic inheritance in an allotetraploid somatic hybrid with gene expression. Carotenoid compounds in the fruit pulp of the two parental species and the hybrid were evaluated quantitatively by HPLC. Only very low levels of beta-carotene and beta-cryptoxanthin were observed in Citrus limon, while beta-cryptoxanthin was a major component of C. reticulata, which also displayed high levels of phytoene, phytofluene, beta-carotene, lutein, zeaxantin and violaxanthin. Total carotenoid content in mandarin juice sacs was 60 times greater than that in lemon. The allotetraploid hybrid produced all the same compounds as mandarin but at very low levels. Transgressive concentration of abscisic acid (ABA) was observed in the somatic hybrid. Real-time RT-PCR of total RNA from juice sacs was used to study expression of seven genes (CitDxs, CitPsy, CitPds, CitZds, CitLcy-b, CitChx-b, and CitZep) of the carotenoid biosynthetic pathway and two genes (CitNced1 and CitNced2) involved in abscisic acid synthesis from carotenoid. Gene expression was significantly higher for mandarin than lemon for seven of the nine genes analyzed. Lemon under expression was partially dominant in the somatic hybrid for three upstream steps of the biosynthetic pathway, particularly for CitDxs. Transgressive over expression was observed for the two CitNced genes. A limitation of the upstream steps of the pathway and a downstream higher consumption of carotenoids may explain the phenotype of the somatic hybrid. 2017-06-01T10:11:01Z 2017-06-01T10:11:01Z 2009 NOV article Bassene, J. B., Froelicher, Y., Dhuique-Mayer, C., Mouhaya, W., Ferrer, R. M., Ancillo, G. et al. (2009). Non-additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between C. reticulata and C. limon: the case of pulp carotenoid biosynthesis pathway. Plant Cell Reports, 28(11), 1689-1697. 0721-7714 http://hdl.handle.net/20.500.11939/4791 10.1007/s00299-009-0768-1 https://link.springer.com/article/10.1007%2Fs00299-009-0768-1 en openAccess Impreso |
| spellingShingle | Bassene, Jean Baptiste Froelicher, Yann Dhuique-Mayer, Claudie Mouhaya, Waffa Ferrer, Rosa M. Ancillo, Gema Morillon, Raphael Navarro, Luis Ollitrault, Patrick Non-additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between C. reticulata and C. limon: the case of pulp carotenoid biosynthesis pathway |
| title | Non-additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between C. reticulata and C. limon: the case of pulp carotenoid biosynthesis pathway |
| title_full | Non-additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between C. reticulata and C. limon: the case of pulp carotenoid biosynthesis pathway |
| title_fullStr | Non-additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between C. reticulata and C. limon: the case of pulp carotenoid biosynthesis pathway |
| title_full_unstemmed | Non-additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between C. reticulata and C. limon: the case of pulp carotenoid biosynthesis pathway |
| title_short | Non-additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between C. reticulata and C. limon: the case of pulp carotenoid biosynthesis pathway |
| title_sort | non additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between c reticulata and c limon the case of pulp carotenoid biosynthesis pathway |
| url | http://hdl.handle.net/20.500.11939/4791 https://link.springer.com/article/10.1007%2Fs00299-009-0768-1 |
| work_keys_str_mv | AT bassenejeanbaptiste nonadditivephenotypicandtranscriptomicinheritanceinacitrusallotetraploidsomatichybridbetweencreticulataandclimonthecaseofpulpcarotenoidbiosynthesispathway AT froelicheryann nonadditivephenotypicandtranscriptomicinheritanceinacitrusallotetraploidsomatichybridbetweencreticulataandclimonthecaseofpulpcarotenoidbiosynthesispathway AT dhuiquemayerclaudie nonadditivephenotypicandtranscriptomicinheritanceinacitrusallotetraploidsomatichybridbetweencreticulataandclimonthecaseofpulpcarotenoidbiosynthesispathway AT mouhayawaffa nonadditivephenotypicandtranscriptomicinheritanceinacitrusallotetraploidsomatichybridbetweencreticulataandclimonthecaseofpulpcarotenoidbiosynthesispathway AT ferrerrosam nonadditivephenotypicandtranscriptomicinheritanceinacitrusallotetraploidsomatichybridbetweencreticulataandclimonthecaseofpulpcarotenoidbiosynthesispathway AT ancillogema nonadditivephenotypicandtranscriptomicinheritanceinacitrusallotetraploidsomatichybridbetweencreticulataandclimonthecaseofpulpcarotenoidbiosynthesispathway AT morillonraphael nonadditivephenotypicandtranscriptomicinheritanceinacitrusallotetraploidsomatichybridbetweencreticulataandclimonthecaseofpulpcarotenoidbiosynthesispathway AT navarroluis nonadditivephenotypicandtranscriptomicinheritanceinacitrusallotetraploidsomatichybridbetweencreticulataandclimonthecaseofpulpcarotenoidbiosynthesispathway AT ollitraultpatrick nonadditivephenotypicandtranscriptomicinheritanceinacitrusallotetraploidsomatichybridbetweencreticulataandclimonthecaseofpulpcarotenoidbiosynthesispathway |