Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus
Citrus leaf blotch virus (CLBV) has a single-stranded, positive-sense, genomic RNA (gRNA) organized in three ORFs, which encode a polyprotein involved in replication (RID), a potential movement protein (MP), and coat protein (CP). Northern blot hybridization of total, virion, or double-stranded RNA...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4768 |
| _version_ | 1855032173372899328 |
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| author | Vives, María C. Galipienso, Luis Navarro, Luis Moreno, Pedro Guerri, José |
| author_browse | Galipienso, Luis Guerri, José Moreno, Pedro Navarro, Luis Vives, María C. |
| author_facet | Vives, María C. Galipienso, Luis Navarro, Luis Moreno, Pedro Guerri, José |
| author_sort | Vives, María C. |
| collection | ReDivia |
| description | Citrus leaf blotch virus (CLBV) has a single-stranded, positive-sense, genomic RNA (gRNA) organized in three ORFs, which encode a polyprotein involved in replication (RID), a potential movement protein (MP), and coat protein (CP). Northern blot hybridization of total, virion, or double-stranded RNA with probes of different gRNA regions revealed that CLBV produces two 3'-coterminal and two 5'-coterminal subgenomic RNAs (sgRNAs). The 3'-coterminal sgRNAs contain the MP (3'MP sgRNA) and CP (3'CP sgRNA) genes and untranslated regions (UTRs) of 123 and 284 nt, respectively, at their 5' end. These sgRNAs start with a hexanucleotide which is also present at the 5' terminus of the gRNA. The 5'-coterminal sgRNAs; have 6795 and 5798 nt, colinear with the gRNA, and contain ORF1 and most MP gene (5'RPMP sgRNA) and most ORF1 (5'RP sgRNA), respectively. Their 3' termini map 35 and 40 nt upstream of the transcription initiation of the 3'CP and 3'MP sgRNAs, respectively, next to a potential promoter element. Our results suggest that, as in alphaviruses, CLBV internal genes are expressed via 3'-coterminal sgRNAs transcribed from the minus gRNA strand, The 5'-coterminal sgRNAs may result from early termination of the gRNA during the plus-strand synthesis, (C) 2002 Elsevier Science (USA). |
| format | article |
| id | ReDivia4768 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia47682025-04-25T14:44:21Z Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus Vives, María C. Galipienso, Luis Navarro, Luis Moreno, Pedro Guerri, José Citrus leaf blotch virus (CLBV) has a single-stranded, positive-sense, genomic RNA (gRNA) organized in three ORFs, which encode a polyprotein involved in replication (RID), a potential movement protein (MP), and coat protein (CP). Northern blot hybridization of total, virion, or double-stranded RNA with probes of different gRNA regions revealed that CLBV produces two 3'-coterminal and two 5'-coterminal subgenomic RNAs (sgRNAs). The 3'-coterminal sgRNAs contain the MP (3'MP sgRNA) and CP (3'CP sgRNA) genes and untranslated regions (UTRs) of 123 and 284 nt, respectively, at their 5' end. These sgRNAs start with a hexanucleotide which is also present at the 5' terminus of the gRNA. The 5'-coterminal sgRNAs; have 6795 and 5798 nt, colinear with the gRNA, and contain ORF1 and most MP gene (5'RPMP sgRNA) and most ORF1 (5'RP sgRNA), respectively. Their 3' termini map 35 and 40 nt upstream of the transcription initiation of the 3'CP and 3'MP sgRNAs, respectively, next to a potential promoter element. Our results suggest that, as in alphaviruses, CLBV internal genes are expressed via 3'-coterminal sgRNAs transcribed from the minus gRNA strand, The 5'-coterminal sgRNAs may result from early termination of the gRNA during the plus-strand synthesis, (C) 2002 Elsevier Science (USA). 2017-06-01T10:10:57Z 2017-06-01T10:10:57Z 2002 APR 10 article Vives, MC, Galipienso, L., Navarro, L., Moreno, P., Guerri, J. (2002). Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus. Virology, 295(2), 328-336. 0042-6822 http://hdl.handle.net/20.500.11939/4768 10.1006/viro.2001.1349 en openAccess Impreso |
| spellingShingle | Vives, María C. Galipienso, Luis Navarro, Luis Moreno, Pedro Guerri, José Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus |
| title | Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus |
| title_full | Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus |
| title_fullStr | Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus |
| title_full_unstemmed | Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus |
| title_short | Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus |
| title_sort | characterization of two kinds of subgenomic rnas produced by citrus leaf blotch virus |
| url | http://hdl.handle.net/20.500.11939/4768 |
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