The Complete Genome Sequence of Citrus Vein Enation Virus (CVEV) Obtained through Deep Sequencing of Small RNAs
Citrus vein enation (VE), a graft-transmissible disease naturally spread by several aphid species in a persistent mode, has been reported in many citrus growing areas. It causes vein enations on leaves and woody galls on trunk and branches of sensitive citrus species such as Mexican lime, rough lemo...
| Main Authors: | , , , , , |
|---|---|
| Other Authors: | |
| Format: | Objeto de conferencia |
| Language: | Inglés |
| Published: |
2017
|
| Online Access: | http://hdl.handle.net/20.500.11939/4766 |
| _version_ | 1855491810190688256 |
|---|---|
| author | Vives, María C. Velázquez, Karelia Pina, José A. Moreno, Pedro Guerri, José Navarro, Luis |
| author2 | Sabater-Munoz, Beatriz |
| author_browse | Guerri, José Moreno, Pedro Navarro, Luis Pina, José A. Sabater-Munoz, Beatriz Velázquez, Karelia Vives, María C. |
| author_facet | Sabater-Munoz, Beatriz Vives, María C. Velázquez, Karelia Pina, José A. Moreno, Pedro Guerri, José Navarro, Luis |
| author_sort | Vives, María C. |
| collection | ReDivia |
| description | Citrus vein enation (VE), a graft-transmissible disease naturally spread by several aphid species in a persistent mode, has been reported in many citrus growing areas. It causes vein enations on leaves and woody galls on trunk and branches of sensitive citrus species such as Mexican lime, rough lemon and Citrus volkameriana. The disease is currently diagnosed by biological indexing on sensitive indicator plants, an expensive and time-consuming method. In order to identify its causal agent and develop specific and reliable molecular detection methods, we analyzed small RNAs (sRNAs) from VE-infected Etrog citron plants by deep sequencing using the Illumina Solexa platform. Assembly of VE-associated sRNAs yielded several contigs that showed sequence homology with Pea enation mosaic virus 1 (PEMV-1), the type species of genus Enamovirus, family Luteoviridae. The gaps between adjacent contigs were filled by RT-PCR amplification, cloning and sequencing in order to obtain the complete genome sequence of a new virus, Citrus vein enation virus (CVEV). The CVEV genomic RNA has 5,983 nt organized in five open reading frames, resembling that of PEMV-1. Phylogenetic comparison of amino acid signatures in RNA-dependent RNA polymerases of the family Luteoviridae clearly grouped CVEV with PEMV-1. Therefore, we propose that CVEV should be included in the genus Enamovirus. A rapid and specific detection procedure was developed based on RT-PCR with CVEV-specific primers. |
| format | Objeto de conferencia |
| id | ReDivia4766 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia47662025-04-25T14:52:39Z The Complete Genome Sequence of Citrus Vein Enation Virus (CVEV) Obtained through Deep Sequencing of Small RNAs Acta Horticulturae Vives, María C. Velázquez, Karelia Pina, José A. Moreno, Pedro Guerri, José Navarro, Luis Sabater-Munoz, Beatriz Moreno, Pedro Pena, Leandro Navarro, Luis Citrus vein enation (VE), a graft-transmissible disease naturally spread by several aphid species in a persistent mode, has been reported in many citrus growing areas. It causes vein enations on leaves and woody galls on trunk and branches of sensitive citrus species such as Mexican lime, rough lemon and Citrus volkameriana. The disease is currently diagnosed by biological indexing on sensitive indicator plants, an expensive and time-consuming method. In order to identify its causal agent and develop specific and reliable molecular detection methods, we analyzed small RNAs (sRNAs) from VE-infected Etrog citron plants by deep sequencing using the Illumina Solexa platform. Assembly of VE-associated sRNAs yielded several contigs that showed sequence homology with Pea enation mosaic virus 1 (PEMV-1), the type species of genus Enamovirus, family Luteoviridae. The gaps between adjacent contigs were filled by RT-PCR amplification, cloning and sequencing in order to obtain the complete genome sequence of a new virus, Citrus vein enation virus (CVEV). The CVEV genomic RNA has 5,983 nt organized in five open reading frames, resembling that of PEMV-1. Phylogenetic comparison of amino acid signatures in RNA-dependent RNA polymerases of the family Luteoviridae clearly grouped CVEV with PEMV-1. Therefore, we propose that CVEV should be included in the genus Enamovirus. A rapid and specific detection procedure was developed based on RT-PCR with CVEV-specific primers. 2017-06-01T10:10:57Z 2017-06-01T10:10:57Z 2015 2015 conferenceObject Vives, M. C., Velazquez, K., Pina, J. A., Moreno, P., Guerri, J. & Navarro, L. (2015). The Complete Genome Sequence of Citrus Vein Enation Virus (CVEV), Obtained through Deep Sequencing of Small RNAs. Acta Horticulturae, 1065, 809-816. 0567-7572; 978-94-62610-53-8 http://hdl.handle.net/20.500.11939/4766 10.17660/ActaHortic.2015.1065.101 en openAccess Impreso |
| spellingShingle | Vives, María C. Velázquez, Karelia Pina, José A. Moreno, Pedro Guerri, José Navarro, Luis The Complete Genome Sequence of Citrus Vein Enation Virus (CVEV) Obtained through Deep Sequencing of Small RNAs |
| title | The Complete Genome Sequence of Citrus Vein Enation Virus (CVEV) Obtained through Deep Sequencing of Small RNAs |
| title_full | The Complete Genome Sequence of Citrus Vein Enation Virus (CVEV) Obtained through Deep Sequencing of Small RNAs |
| title_fullStr | The Complete Genome Sequence of Citrus Vein Enation Virus (CVEV) Obtained through Deep Sequencing of Small RNAs |
| title_full_unstemmed | The Complete Genome Sequence of Citrus Vein Enation Virus (CVEV) Obtained through Deep Sequencing of Small RNAs |
| title_short | The Complete Genome Sequence of Citrus Vein Enation Virus (CVEV) Obtained through Deep Sequencing of Small RNAs |
| title_sort | complete genome sequence of citrus vein enation virus cvev obtained through deep sequencing of small rnas |
| url | http://hdl.handle.net/20.500.11939/4766 |
| work_keys_str_mv | AT vivesmariac thecompletegenomesequenceofcitrusveinenationviruscvevobtainedthroughdeepsequencingofsmallrnas AT velazquezkarelia thecompletegenomesequenceofcitrusveinenationviruscvevobtainedthroughdeepsequencingofsmallrnas AT pinajosea thecompletegenomesequenceofcitrusveinenationviruscvevobtainedthroughdeepsequencingofsmallrnas AT morenopedro thecompletegenomesequenceofcitrusveinenationviruscvevobtainedthroughdeepsequencingofsmallrnas AT guerrijose thecompletegenomesequenceofcitrusveinenationviruscvevobtainedthroughdeepsequencingofsmallrnas AT navarroluis thecompletegenomesequenceofcitrusveinenationviruscvevobtainedthroughdeepsequencingofsmallrnas AT vivesmariac actahorticulturae AT velazquezkarelia actahorticulturae AT pinajosea actahorticulturae AT morenopedro actahorticulturae AT guerrijose actahorticulturae AT navarroluis actahorticulturae AT vivesmariac completegenomesequenceofcitrusveinenationviruscvevobtainedthroughdeepsequencingofsmallrnas AT velazquezkarelia completegenomesequenceofcitrusveinenationviruscvevobtainedthroughdeepsequencingofsmallrnas AT pinajosea completegenomesequenceofcitrusveinenationviruscvevobtainedthroughdeepsequencingofsmallrnas AT morenopedro completegenomesequenceofcitrusveinenationviruscvevobtainedthroughdeepsequencingofsmallrnas AT guerrijose completegenomesequenceofcitrusveinenationviruscvevobtainedthroughdeepsequencingofsmallrnas AT navarroluis completegenomesequenceofcitrusveinenationviruscvevobtainedthroughdeepsequencingofsmallrnas |