Determination of Enzyme Activity in Rabbit Seminal Plasma and its Relationship with Quality Semen Parameters
The objective of this study was to determine rabbit seminal plasma enzyme activity. Furthermore, correlations between semen parameters and enzyme activity and male age were examined. The study was performed using 17 New Zealand White males from 5 to 9 mo old. Overall, 252 semen samples were collecte...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4757 |
| _version_ | 1855032171408916480 |
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| author | Viudes-De-Castro, María P. Casares-Crespo, Lucía Monserrat-Martinez, A. Vicente, José Salvador |
| author_browse | Casares-Crespo, Lucía Monserrat-Martinez, A. Vicente, José Salvador Viudes-De-Castro, María P. |
| author_facet | Viudes-De-Castro, María P. Casares-Crespo, Lucía Monserrat-Martinez, A. Vicente, José Salvador |
| author_sort | Viudes-De-Castro, María P. |
| collection | ReDivia |
| description | The objective of this study was to determine rabbit seminal plasma enzyme activity. Furthermore, correlations between semen parameters and enzyme activity and male age were examined. The study was performed using 17 New Zealand White males from 5 to 9 mo old. Overall, 252 semen samples were collected from bucks from May to September. Semen characteristics were analysed and the seminal plasma was obtained by centrifugation. The activities of alanyl aminopeptidase (APN), aspartate transaminase (AST), alanine aminotransferase (ALT), gamma-glutamyl transpeptidase (GOT), lactate dehydrogenase (LDH) and alkaline phosphatase (ALKP) in the seminal plasma fluid were measured. Significant differences between males were found in APN, GGT, LDH, ALKP and ALT activities (P<0.05). No significant differences between enzyme activity and male age were found. We also observed significant positive correlations between male age and sperm concentration (r=0.26), progressive motility (r=0.17) and amplitude of lateral head displacement (r=0.21), and negative ones between male age and average path velocity (r=-0.56), velocity of the sperm head along its actual curvilinear path (r=-0.61), straight line velocity (r=-0.50), linearity index (r=-0.13), and cytoplasmic droplet (r=-0.33). Furthermore, a significant negative correlation between APN activity and the status of the acrosome (r=-0.20) and significant positive correlations between APN activity and the sperm abnormalities (r=0.21), GOT activity and sperm concentration (r=0.34) and the status of the acrosome (r=0.31), and ALKP activity and sperm concentration were observed (r=0.41). In our study, APN and GGT seem to be the most predictive enzymes for rabbit semen quality. |
| format | article |
| id | ReDivia4757 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia47572025-04-25T14:44:19Z Determination of Enzyme Activity in Rabbit Seminal Plasma and its Relationship with Quality Semen Parameters Viudes-De-Castro, María P. Casares-Crespo, Lucía Monserrat-Martinez, A. Vicente, José Salvador The objective of this study was to determine rabbit seminal plasma enzyme activity. Furthermore, correlations between semen parameters and enzyme activity and male age were examined. The study was performed using 17 New Zealand White males from 5 to 9 mo old. Overall, 252 semen samples were collected from bucks from May to September. Semen characteristics were analysed and the seminal plasma was obtained by centrifugation. The activities of alanyl aminopeptidase (APN), aspartate transaminase (AST), alanine aminotransferase (ALT), gamma-glutamyl transpeptidase (GOT), lactate dehydrogenase (LDH) and alkaline phosphatase (ALKP) in the seminal plasma fluid were measured. Significant differences between males were found in APN, GGT, LDH, ALKP and ALT activities (P<0.05). No significant differences between enzyme activity and male age were found. We also observed significant positive correlations between male age and sperm concentration (r=0.26), progressive motility (r=0.17) and amplitude of lateral head displacement (r=0.21), and negative ones between male age and average path velocity (r=-0.56), velocity of the sperm head along its actual curvilinear path (r=-0.61), straight line velocity (r=-0.50), linearity index (r=-0.13), and cytoplasmic droplet (r=-0.33). Furthermore, a significant negative correlation between APN activity and the status of the acrosome (r=-0.20) and significant positive correlations between APN activity and the sperm abnormalities (r=0.21), GOT activity and sperm concentration (r=0.34) and the status of the acrosome (r=0.31), and ALKP activity and sperm concentration were observed (r=0.41). In our study, APN and GGT seem to be the most predictive enzymes for rabbit semen quality. 2017-06-01T10:10:55Z 2017-06-01T10:10:55Z 2015 OCT-DEC 2015 article publishedVersion Viudes-De-Castro, M.P., Casares-Crespo, L., Monserrat-Martinez, A., Vicente, J.S. (2015). Determination of Enzyme Activity in Rabbit Seminal Plasma and its Relationship with Quality Semen Parameters. World Rabbit Science, 23(4), 247-253. 1257-5011 http://hdl.handle.net/20.500.11939/4757 http://hdl.handle.net/20.500.11939/4757 10.4995/wrs.2015.4064 en openAccess Impreso |
| spellingShingle | Viudes-De-Castro, María P. Casares-Crespo, Lucía Monserrat-Martinez, A. Vicente, José Salvador Determination of Enzyme Activity in Rabbit Seminal Plasma and its Relationship with Quality Semen Parameters |
| title | Determination of Enzyme Activity in Rabbit Seminal Plasma and its Relationship with Quality Semen Parameters |
| title_full | Determination of Enzyme Activity in Rabbit Seminal Plasma and its Relationship with Quality Semen Parameters |
| title_fullStr | Determination of Enzyme Activity in Rabbit Seminal Plasma and its Relationship with Quality Semen Parameters |
| title_full_unstemmed | Determination of Enzyme Activity in Rabbit Seminal Plasma and its Relationship with Quality Semen Parameters |
| title_short | Determination of Enzyme Activity in Rabbit Seminal Plasma and its Relationship with Quality Semen Parameters |
| title_sort | determination of enzyme activity in rabbit seminal plasma and its relationship with quality semen parameters |
| url | http://hdl.handle.net/20.500.11939/4757 http://hdl.handle.net/20.500.11939/4757 |
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