Development of SSR markers located in the G1 linkage group of apricot (Prunus armeniaca L.) using a bacterial artificial chromosome library
Sixteen simple sequence repeats (SSRs) of apricot (Prunus armeniaca L.) were isolated from a bacterial artificial chromosome (BAC) library. Twelve restriction fragment length polymorphism (RFLP) probes mapped on LG1 of the Prunus general map were hybridized to the BAC library in order to select clon...
| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4729 |
| _version_ | 1855032167981121536 |
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| author | Vilanova, S. Soriano, J. M. Lalli, D. A. Romero, Carlos Abbott, Albert Glenn Llácer, Gerardo Badenes, María L. |
| author_browse | Abbott, Albert Glenn Badenes, María L. Lalli, D. A. Llácer, Gerardo Romero, Carlos Soriano, J. M. Vilanova, S. |
| author_facet | Vilanova, S. Soriano, J. M. Lalli, D. A. Romero, Carlos Abbott, Albert Glenn Llácer, Gerardo Badenes, María L. |
| author_sort | Vilanova, S. |
| collection | ReDivia |
| description | Sixteen simple sequence repeats (SSRs) of apricot (Prunus armeniaca L.) were isolated from a bacterial artificial chromosome (BAC) library. Twelve restriction fragment length polymorphism (RFLP) probes mapped on LG1 of the Prunus general map were hybridized to the BAC library in order to select clones belonging to G1 linkage group of apricot. Selected BACs were digested, subcloned and hybridized with probes containing repeat motifs (GA)(10) and (TA)(10). Sequencing of the positive subclones revealed 18 unique SSR sequences of which 16 allowed the design of primers flanking the SSR. From the 16 primer pairs, 10 amplified polymorphic markers with an average of observed and expected heterozygosities of 0.44 and 0.68, respectively. The procedure described here proves to be a useful technique for obtaining markers in target areas of a genome. |
| format | article |
| id | ReDivia4729 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia47292025-04-25T14:44:16Z Development of SSR markers located in the G1 linkage group of apricot (Prunus armeniaca L.) using a bacterial artificial chromosome library Vilanova, S. Soriano, J. M. Lalli, D. A. Romero, Carlos Abbott, Albert Glenn Llácer, Gerardo Badenes, María L. Sixteen simple sequence repeats (SSRs) of apricot (Prunus armeniaca L.) were isolated from a bacterial artificial chromosome (BAC) library. Twelve restriction fragment length polymorphism (RFLP) probes mapped on LG1 of the Prunus general map were hybridized to the BAC library in order to select clones belonging to G1 linkage group of apricot. Selected BACs were digested, subcloned and hybridized with probes containing repeat motifs (GA)(10) and (TA)(10). Sequencing of the positive subclones revealed 18 unique SSR sequences of which 16 allowed the design of primers flanking the SSR. From the 16 primer pairs, 10 amplified polymorphic markers with an average of observed and expected heterozygosities of 0.44 and 0.68, respectively. The procedure described here proves to be a useful technique for obtaining markers in target areas of a genome. 2017-06-01T10:10:51Z 2017-06-01T10:10:51Z 2006 SEP 2006 article Vilanova, S., Soriano, J. M., Lalli, D. A., Romero, C., Abbott, A. G., Llacer, G. et al. (2006). Development of SSR markers located in the G1 linkage group of apricot (Prunus armeniaca L.) using a bacterial artificial chromosome library. Molecular Ecology Notes, 6(3), 789-791. 1471-8278 http://hdl.handle.net/20.500.11939/4729 10.1111/j.1471-8286.2006.01346.x en openAccess Impreso |
| spellingShingle | Vilanova, S. Soriano, J. M. Lalli, D. A. Romero, Carlos Abbott, Albert Glenn Llácer, Gerardo Badenes, María L. Development of SSR markers located in the G1 linkage group of apricot (Prunus armeniaca L.) using a bacterial artificial chromosome library |
| title | Development of SSR markers located in the G1 linkage group of apricot (Prunus armeniaca L.) using a bacterial artificial chromosome library |
| title_full | Development of SSR markers located in the G1 linkage group of apricot (Prunus armeniaca L.) using a bacterial artificial chromosome library |
| title_fullStr | Development of SSR markers located in the G1 linkage group of apricot (Prunus armeniaca L.) using a bacterial artificial chromosome library |
| title_full_unstemmed | Development of SSR markers located in the G1 linkage group of apricot (Prunus armeniaca L.) using a bacterial artificial chromosome library |
| title_short | Development of SSR markers located in the G1 linkage group of apricot (Prunus armeniaca L.) using a bacterial artificial chromosome library |
| title_sort | development of ssr markers located in the g1 linkage group of apricot prunus armeniaca l using a bacterial artificial chromosome library |
| url | http://hdl.handle.net/20.500.11939/4729 |
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