Self-(in)compatibility in Prunus armeniaca L.: Analysis of the S-locus structure and identification of S-haplotype specific S-RNase
The gametophytic self-incompatibility (GSI) system in Rosaceae has been proposed to be controlled by two genes located in the S-locus: an S-RNase and a recently-described, pollen-expressed, S-haplotype-specific F-box gene (SFB). Fragments containing genes homologous to the S-RNase and F-box genes in...
| Autores principales: | , , , , , , |
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| Formato: | conferenceObject |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4728 https://doi.org/10.17660/ActaHortic.2006.717.44 |
| _version_ | 1855032167808106496 |
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| author | Vilanova, S. Romero, Carlos Martínez-Calvo, José Vicente, M. Llácer, Gerardo Badenes, María L. Burgos, Lorenzo |
| author2 | Romojaro, Félix |
| author_browse | Badenes, María L. Burgos, Lorenzo Llácer, Gerardo Martínez-Calvo, José Romero, Carlos Romojaro, Félix Vicente, M. Vilanova, S. |
| author_facet | Romojaro, Félix Vilanova, S. Romero, Carlos Martínez-Calvo, José Vicente, M. Llácer, Gerardo Badenes, María L. Burgos, Lorenzo |
| author_sort | Vilanova, S. |
| collection | ReDivia |
| description | The gametophytic self-incompatibility (GSI) system in Rosaceae has been proposed to be controlled by two genes located in the S-locus: an S-RNase and a recently-described, pollen-expressed, S-haplotype-specific F-box gene (SFB). Fragments containing genes homologous to the S-RNase and F-box genes in Prunus species have been described. In apricot (Prunus armeniaca L.), these genes were identified recently. Three identified S-RNase alleles and the F-box allelic variants (SFB1, SFB2 and SFB4) allowed elucidation of the S-locus structure in apricot. In this paper, from the analysis of the S-locus, we carried out the molecular typing of several S-alleles in apricot. A primer pair developed from conserved regions identified in the apricot S-locus was used to amplify the first S-RNase intron. Fragments containing the second intron were obtained using two sets of primers designed from published Prunus avium S-cDNA sequences. These results confirm genotypes established by other methods and establish five self-(in)compatibility groups in apricot. |
| format | conferenceObject |
| id | ReDivia4728 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia47282025-04-25T14:53:40Z Self-(in)compatibility in Prunus armeniaca L.: Analysis of the S-locus structure and identification of S-haplotype specific S-RNase ACTA HORTICULTURAE Vilanova, S. Romero, Carlos Martínez-Calvo, José Vicente, M. Llácer, Gerardo Badenes, María L. Burgos, Lorenzo Romojaro, Félix The gametophytic self-incompatibility (GSI) system in Rosaceae has been proposed to be controlled by two genes located in the S-locus: an S-RNase and a recently-described, pollen-expressed, S-haplotype-specific F-box gene (SFB). Fragments containing genes homologous to the S-RNase and F-box genes in Prunus species have been described. In apricot (Prunus armeniaca L.), these genes were identified recently. Three identified S-RNase alleles and the F-box allelic variants (SFB1, SFB2 and SFB4) allowed elucidation of the S-locus structure in apricot. In this paper, from the analysis of the S-locus, we carried out the molecular typing of several S-alleles in apricot. A primer pair developed from conserved regions identified in the apricot S-locus was used to amplify the first S-RNase intron. Fragments containing the second intron were obtained using two sets of primers designed from published Prunus avium S-cDNA sequences. These results confirm genotypes established by other methods and establish five self-(in)compatibility groups in apricot. 2017-06-01T10:10:51Z 2017-06-01T10:10:51Z 2006 2006 conferenceObject Vilanova, S., Romero, C., Martinez-Calvo, J., Vicente, M., Llacer, G., Badenes, M. L. et al. (2006). Self-(in)compatibility in Prunus armeniaca L.: Analysis of the S-locus structure and identification of S-haplotype specific S-RNase. Acta horticulturae, 717, 213-216. 0567-7572; 90-6605-599-5 http://hdl.handle.net/20.500.11939/4728 https://doi.org/10.17660/ActaHortic.2006.717.44 en openAccess Impreso |
| spellingShingle | Vilanova, S. Romero, Carlos Martínez-Calvo, José Vicente, M. Llácer, Gerardo Badenes, María L. Burgos, Lorenzo Self-(in)compatibility in Prunus armeniaca L.: Analysis of the S-locus structure and identification of S-haplotype specific S-RNase |
| title | Self-(in)compatibility in Prunus armeniaca L.: Analysis of the S-locus structure and identification of S-haplotype specific S-RNase |
| title_full | Self-(in)compatibility in Prunus armeniaca L.: Analysis of the S-locus structure and identification of S-haplotype specific S-RNase |
| title_fullStr | Self-(in)compatibility in Prunus armeniaca L.: Analysis of the S-locus structure and identification of S-haplotype specific S-RNase |
| title_full_unstemmed | Self-(in)compatibility in Prunus armeniaca L.: Analysis of the S-locus structure and identification of S-haplotype specific S-RNase |
| title_short | Self-(in)compatibility in Prunus armeniaca L.: Analysis of the S-locus structure and identification of S-haplotype specific S-RNase |
| title_sort | self in compatibility in prunus armeniaca l analysis of the s locus structure and identification of s haplotype specific s rnase |
| url | http://hdl.handle.net/20.500.11939/4728 https://doi.org/10.17660/ActaHortic.2006.717.44 |
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