Estimation of the accuracy of two diagnostic methods for the detection of Plum pox virus in nursery blocks by latent class models
The control of Plum pox virus (PPV), the most important viral disease that affects stone fruit trees, requires the use of reliable detection methods. The effectiveness of spot real-time reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of PPV in samples collected from nurser...
| Main Authors: | , , , |
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| Format: | article |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/4719 |
| _version_ | 1855032166215319552 |
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| author | Vidal, Eduardo Moreno, A. Bertolini, Edson Cambra, Mariano |
| author_browse | Bertolini, Edson Cambra, Mariano Moreno, A. Vidal, Eduardo |
| author_facet | Vidal, Eduardo Moreno, A. Bertolini, Edson Cambra, Mariano |
| author_sort | Vidal, Eduardo |
| collection | ReDivia |
| description | The control of Plum pox virus (PPV), the most important viral disease that affects stone fruit trees, requires the use of reliable detection methods. The effectiveness of spot real-time reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of PPV in samples collected from nursery blocks was compared with a validated PPV detection technique, the double antibody sandwich indirect enzyme-linked immunosorbent assay (DASI-ELISA) using the PPV-specific monoclonal antibody 5B-IVIA/AMR. In total, 5047 nursery plants were analysed by both techniques. The agreement between the techniques was almost perfect (Cohens kappa index of 0.88 +/- 0.01). The diagnostic parameters (sensitivity, specificity and likelihood ratios) of both techniques were simultaneously evaluated in 2473 nursery plants by latent class models using maximum likelihood functions and a Bayesian approach. The sensitivity and specificity of both techniques did not vary according to the latent model applied. Spot real-time RT-PCR was more sensitive while DASI-ELISA was more specific for PPV detection. In addition, the findings demonstrate that latent class models are a flexible and potent statistical method to estimate the accuracy of diagnostic tests for plant pathology. |
| format | article |
| id | ReDivia4719 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia47192025-04-25T14:44:15Z Estimation of the accuracy of two diagnostic methods for the detection of Plum pox virus in nursery blocks by latent class models Vidal, Eduardo Moreno, A. Bertolini, Edson Cambra, Mariano The control of Plum pox virus (PPV), the most important viral disease that affects stone fruit trees, requires the use of reliable detection methods. The effectiveness of spot real-time reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of PPV in samples collected from nursery blocks was compared with a validated PPV detection technique, the double antibody sandwich indirect enzyme-linked immunosorbent assay (DASI-ELISA) using the PPV-specific monoclonal antibody 5B-IVIA/AMR. In total, 5047 nursery plants were analysed by both techniques. The agreement between the techniques was almost perfect (Cohens kappa index of 0.88 +/- 0.01). The diagnostic parameters (sensitivity, specificity and likelihood ratios) of both techniques were simultaneously evaluated in 2473 nursery plants by latent class models using maximum likelihood functions and a Bayesian approach. The sensitivity and specificity of both techniques did not vary according to the latent model applied. Spot real-time RT-PCR was more sensitive while DASI-ELISA was more specific for PPV detection. In addition, the findings demonstrate that latent class models are a flexible and potent statistical method to estimate the accuracy of diagnostic tests for plant pathology. 2017-06-01T10:10:50Z 2017-06-01T10:10:50Z 2012 APR 2012 article Vidal, E., Moreno, A., Bertolini, E., Cambra, M. (2012). Estimation of the accuracy of two diagnostic methods for the detection of Plum pox virus in nursery blocks by latent class models. Plant Pathology, 61(2), 413-422. 0032-0862 http://hdl.handle.net/20.500.11939/4719 10.1111/j.1365-3059.2011.02505.x en openAccess Impreso |
| spellingShingle | Vidal, Eduardo Moreno, A. Bertolini, Edson Cambra, Mariano Estimation of the accuracy of two diagnostic methods for the detection of Plum pox virus in nursery blocks by latent class models |
| title | Estimation of the accuracy of two diagnostic methods for the detection of Plum pox virus in nursery blocks by latent class models |
| title_full | Estimation of the accuracy of two diagnostic methods for the detection of Plum pox virus in nursery blocks by latent class models |
| title_fullStr | Estimation of the accuracy of two diagnostic methods for the detection of Plum pox virus in nursery blocks by latent class models |
| title_full_unstemmed | Estimation of the accuracy of two diagnostic methods for the detection of Plum pox virus in nursery blocks by latent class models |
| title_short | Estimation of the accuracy of two diagnostic methods for the detection of Plum pox virus in nursery blocks by latent class models |
| title_sort | estimation of the accuracy of two diagnostic methods for the detection of plum pox virus in nursery blocks by latent class models |
| url | http://hdl.handle.net/20.500.11939/4719 |
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