Adaptation of Staphylococcus aureus to ruminant and equine hosts involves SaPI-carried variants of von Willebrand factor-binding protein
Staphylococci adapt specifically to various animal hosts by genetically determined mechanisms that are not well understood. One such adaptation involves the ability to coagulate host plasma, by which strains isolated from ruminants or horses can be differentiated from closely related human strains....
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/4703 |
| _version_ | 1855032163696640000 |
|---|---|
| author | Viana, David Blanco, Jose Tormo-Mas, María A. Selva, Laura Guinane, Caitriona M. Baselga, Rafael Corpa, Juan M. Lasa, Inigo Novick, Richard P. Fitzgerald, J. Ross Penadés, José R. |
| author_browse | Baselga, Rafael Blanco, Jose Corpa, Juan M. Fitzgerald, J. Ross Guinane, Caitriona M. Lasa, Inigo Novick, Richard P. Penadés, José R. Selva, Laura Tormo-Mas, María A. Viana, David |
| author_facet | Viana, David Blanco, Jose Tormo-Mas, María A. Selva, Laura Guinane, Caitriona M. Baselga, Rafael Corpa, Juan M. Lasa, Inigo Novick, Richard P. Fitzgerald, J. Ross Penadés, José R. |
| author_sort | Viana, David |
| collection | ReDivia |
| description | Staphylococci adapt specifically to various animal hosts by genetically determined mechanisms that are not well understood. One such adaptation involves the ability to coagulate host plasma, by which strains isolated from ruminants or horses can be differentiated from closely related human strains. Here, we report first that this differential coagulation activity is due to animal-specific alleles of the von Willebrand factor-binding protein (vWbp) gene, vwb, and second that these vwb alleles are carried by highly mobile pathogenicity islands, SaPIs. Although all Staphylococcus aureus possess chromosomal vwb as well as coagulase (coa) genes, neither confers species-specific coagulation activity; however, the SaPI-coded vWbps possess a unique N-terminal region specific for the activation of ruminant and equine prothrombin. vWbp-encoding SaPIs are widely distributed among S. aureus strains infecting ruminant or equine hosts, and we have identified and characterized four of these, SaPIbov4, SaPIbov5, SaPIeq1 and SaPIov2, which encode vWbpSbo4, vWbpSbo5, vWbpSeq1 and vWbpSov2 respectively. Moreover, the SaPI-carried vwb genes are regulated differently from the chromosomal vwb genes of the same strains. We suggest that the SaPI-encoded vWbps may represent an important host adaptation mechanism for S. aureus pathogenicity, and therefore that acquisition of vWbp-encoding SaPIs may be determinative for animal specificity. |
| format | article |
| id | ReDivia4703 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia47032025-04-25T14:44:12Z Adaptation of Staphylococcus aureus to ruminant and equine hosts involves SaPI-carried variants of von Willebrand factor-binding protein Viana, David Blanco, Jose Tormo-Mas, María A. Selva, Laura Guinane, Caitriona M. Baselga, Rafael Corpa, Juan M. Lasa, Inigo Novick, Richard P. Fitzgerald, J. Ross Penadés, José R. Staphylococci adapt specifically to various animal hosts by genetically determined mechanisms that are not well understood. One such adaptation involves the ability to coagulate host plasma, by which strains isolated from ruminants or horses can be differentiated from closely related human strains. Here, we report first that this differential coagulation activity is due to animal-specific alleles of the von Willebrand factor-binding protein (vWbp) gene, vwb, and second that these vwb alleles are carried by highly mobile pathogenicity islands, SaPIs. Although all Staphylococcus aureus possess chromosomal vwb as well as coagulase (coa) genes, neither confers species-specific coagulation activity; however, the SaPI-coded vWbps possess a unique N-terminal region specific for the activation of ruminant and equine prothrombin. vWbp-encoding SaPIs are widely distributed among S. aureus strains infecting ruminant or equine hosts, and we have identified and characterized four of these, SaPIbov4, SaPIbov5, SaPIeq1 and SaPIov2, which encode vWbpSbo4, vWbpSbo5, vWbpSeq1 and vWbpSov2 respectively. Moreover, the SaPI-carried vwb genes are regulated differently from the chromosomal vwb genes of the same strains. We suggest that the SaPI-encoded vWbps may represent an important host adaptation mechanism for S. aureus pathogenicity, and therefore that acquisition of vWbp-encoding SaPIs may be determinative for animal specificity. 2017-06-01T10:10:47Z 2017-06-01T10:10:47Z 2010 SEP 2010 article Viana, David, Blanco, J., Angeles Tormo-Mas, M., Selva, L., Guinane, Caitriona M., Baselga, Rafael, Corpa, J. M., Lasa, Inigo, Novick, Richard P., Fitzgerald, J. Ross, Penades, J.R. (2010). Adaptation of Staphylococcus aureus to ruminant and equine hosts involves SaPI-carried variants of von Willebrand factor-binding protein. Molecular microbiology, 77(6), 1583-1594. 0950-382X http://hdl.handle.net/20.500.11939/4703 10.1111/j.1365-2958.2010.07312.x en openAccess Impreso |
| spellingShingle | Viana, David Blanco, Jose Tormo-Mas, María A. Selva, Laura Guinane, Caitriona M. Baselga, Rafael Corpa, Juan M. Lasa, Inigo Novick, Richard P. Fitzgerald, J. Ross Penadés, José R. Adaptation of Staphylococcus aureus to ruminant and equine hosts involves SaPI-carried variants of von Willebrand factor-binding protein |
| title | Adaptation of Staphylococcus aureus to ruminant and equine hosts involves SaPI-carried variants of von Willebrand factor-binding protein |
| title_full | Adaptation of Staphylococcus aureus to ruminant and equine hosts involves SaPI-carried variants of von Willebrand factor-binding protein |
| title_fullStr | Adaptation of Staphylococcus aureus to ruminant and equine hosts involves SaPI-carried variants of von Willebrand factor-binding protein |
| title_full_unstemmed | Adaptation of Staphylococcus aureus to ruminant and equine hosts involves SaPI-carried variants of von Willebrand factor-binding protein |
| title_short | Adaptation of Staphylococcus aureus to ruminant and equine hosts involves SaPI-carried variants of von Willebrand factor-binding protein |
| title_sort | adaptation of staphylococcus aureus to ruminant and equine hosts involves sapi carried variants of von willebrand factor binding protein |
| url | http://hdl.handle.net/20.500.11939/4703 |
| work_keys_str_mv | AT vianadavid adaptationofstaphylococcusaureustoruminantandequinehostsinvolvessapicarriedvariantsofvonwillebrandfactorbindingprotein AT blancojose adaptationofstaphylococcusaureustoruminantandequinehostsinvolvessapicarriedvariantsofvonwillebrandfactorbindingprotein AT tormomasmariaa adaptationofstaphylococcusaureustoruminantandequinehostsinvolvessapicarriedvariantsofvonwillebrandfactorbindingprotein AT selvalaura adaptationofstaphylococcusaureustoruminantandequinehostsinvolvessapicarriedvariantsofvonwillebrandfactorbindingprotein AT guinanecaitrionam adaptationofstaphylococcusaureustoruminantandequinehostsinvolvessapicarriedvariantsofvonwillebrandfactorbindingprotein AT baselgarafael adaptationofstaphylococcusaureustoruminantandequinehostsinvolvessapicarriedvariantsofvonwillebrandfactorbindingprotein AT corpajuanm adaptationofstaphylococcusaureustoruminantandequinehostsinvolvessapicarriedvariantsofvonwillebrandfactorbindingprotein AT lasainigo adaptationofstaphylococcusaureustoruminantandequinehostsinvolvessapicarriedvariantsofvonwillebrandfactorbindingprotein AT novickrichardp adaptationofstaphylococcusaureustoruminantandequinehostsinvolvessapicarriedvariantsofvonwillebrandfactorbindingprotein AT fitzgeraldjross adaptationofstaphylococcusaureustoruminantandequinehostsinvolvessapicarriedvariantsofvonwillebrandfactorbindingprotein AT penadesjoser adaptationofstaphylococcusaureustoruminantandequinehostsinvolvessapicarriedvariantsofvonwillebrandfactorbindingprotein |