Relevant Role of Fibronectin-Binding Proteins in Staphylococcus aureus Biofilm-Associated Foreign-Body Infections
Staphylococcus aureus can establish chronic infections on implanted medical devices due to its capacity to form biofilms. Analysis of the factors that assemble cells into a biofilm has revealed the occurrence of strains that produce either a polysaccharide intercellular adhesin/poly-N-acetylglucosam...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4701 https://iai.asm.org/content/iai/77/9/3978.full.pdf |
| _version_ | 1855491799805591552 |
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| author | Vergara-Irigaray, Marta Valle, Jaione Merino, Nekane Latasa, Cristina Garcia, Begona Ruiz de los Mozos, Igor Solano, Cristina Toledo-Arana, Alejandro Penadés, José R. Lasa, Inigo |
| author_browse | Garcia, Begona Lasa, Inigo Latasa, Cristina Merino, Nekane Penadés, José R. Ruiz de los Mozos, Igor Solano, Cristina Toledo-Arana, Alejandro Valle, Jaione Vergara-Irigaray, Marta |
| author_facet | Vergara-Irigaray, Marta Valle, Jaione Merino, Nekane Latasa, Cristina Garcia, Begona Ruiz de los Mozos, Igor Solano, Cristina Toledo-Arana, Alejandro Penadés, José R. Lasa, Inigo |
| author_sort | Vergara-Irigaray, Marta |
| collection | ReDivia |
| description | Staphylococcus aureus can establish chronic infections on implanted medical devices due to its capacity to form biofilms. Analysis of the factors that assemble cells into a biofilm has revealed the occurrence of strains that produce either a polysaccharide intercellular adhesin/poly-N-acetylglucosamine (PIA/PNAG) exopolysaccharide- or a protein-dependent biofilm. Examination of the influence of matrix nature on the biofilm capacities of embedded bacteria has remained elusive, because a natural strain that readily converts between a polysaccharide- and a protein-based biofilm has not been studied. Here, we have investigated the clinical methicillin (meticillin)-resistant Staphylococcus aureus strain 132, which is able to alternate between a proteinaceous and an exopolysaccharidic biofilm matrix, depending on environmental conditions. Systematic disruption of each member of the LPXTG surface protein family identified fibronectin-binding proteins (FnBPs) as components of a proteinaceous biofilm formed in Trypticase soy broth-glucose, whereas a PIA/PNAG-dependent biofilm was produced under osmotic stress conditions. The induction of FnBP levels due to a spontaneous agr deficiency present in strain 132 and the activation of a LexA-dependent SOS response or FnBP overexpression from a multicopy plasmid enhanced biofilm development, suggesting a direct relationship between the FnBP levels and the strength of the multicellular phenotype. Scanning electron microscopy revealed that cells growing in the FnBP-mediated biofilm formed highly dense aggregates without any detectable extracellular matrix, whereas cells in a PIA/PNAG-dependent biofilm were embedded in an abundant extracellular material. Finally, studies of the contribution of each type of biofilm matrix to subcutaneous catheter colonization revealed that an FnBP mutant displayed a significantly lower capacity to develop biofilm on implanted catheters than the isogenic PIA/PNAG-deficient mutant. |
| format | Artículo |
| id | ReDivia4701 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia47012025-04-25T14:44:11Z Relevant Role of Fibronectin-Binding Proteins in Staphylococcus aureus Biofilm-Associated Foreign-Body Infections Vergara-Irigaray, Marta Valle, Jaione Merino, Nekane Latasa, Cristina Garcia, Begona Ruiz de los Mozos, Igor Solano, Cristina Toledo-Arana, Alejandro Penadés, José R. Lasa, Inigo Staphylococcus aureus can establish chronic infections on implanted medical devices due to its capacity to form biofilms. Analysis of the factors that assemble cells into a biofilm has revealed the occurrence of strains that produce either a polysaccharide intercellular adhesin/poly-N-acetylglucosamine (PIA/PNAG) exopolysaccharide- or a protein-dependent biofilm. Examination of the influence of matrix nature on the biofilm capacities of embedded bacteria has remained elusive, because a natural strain that readily converts between a polysaccharide- and a protein-based biofilm has not been studied. Here, we have investigated the clinical methicillin (meticillin)-resistant Staphylococcus aureus strain 132, which is able to alternate between a proteinaceous and an exopolysaccharidic biofilm matrix, depending on environmental conditions. Systematic disruption of each member of the LPXTG surface protein family identified fibronectin-binding proteins (FnBPs) as components of a proteinaceous biofilm formed in Trypticase soy broth-glucose, whereas a PIA/PNAG-dependent biofilm was produced under osmotic stress conditions. The induction of FnBP levels due to a spontaneous agr deficiency present in strain 132 and the activation of a LexA-dependent SOS response or FnBP overexpression from a multicopy plasmid enhanced biofilm development, suggesting a direct relationship between the FnBP levels and the strength of the multicellular phenotype. Scanning electron microscopy revealed that cells growing in the FnBP-mediated biofilm formed highly dense aggregates without any detectable extracellular matrix, whereas cells in a PIA/PNAG-dependent biofilm were embedded in an abundant extracellular material. Finally, studies of the contribution of each type of biofilm matrix to subcutaneous catheter colonization revealed that an FnBP mutant displayed a significantly lower capacity to develop biofilm on implanted catheters than the isogenic PIA/PNAG-deficient mutant. 2017-06-01T10:10:47Z 2017-06-01T10:10:47Z 2009 SEP 2009 article Vergara-Irigaray, Marta, Valle, Jaione, Merino, Nekane, Latasa, C., Garcia, Begona, Ruiz de los Mozos, Igor, Solano, C., Toledo-Arana, A., Penades, J.R., Lasa, Inigo (2009). Relevant Role of Fibronectin-Binding Proteins in Staphylococcus aureus Biofilm-Associated Foreign-Body Infections. Infection and immunity, 77(9), 3978-3991. 0019-9567 http://hdl.handle.net/20.500.11939/4701 10.1128/IAI.00616-09 https://iai.asm.org/content/iai/77/9/3978.full.pdf en openAccess Impreso |
| spellingShingle | Vergara-Irigaray, Marta Valle, Jaione Merino, Nekane Latasa, Cristina Garcia, Begona Ruiz de los Mozos, Igor Solano, Cristina Toledo-Arana, Alejandro Penadés, José R. Lasa, Inigo Relevant Role of Fibronectin-Binding Proteins in Staphylococcus aureus Biofilm-Associated Foreign-Body Infections |
| title | Relevant Role of Fibronectin-Binding Proteins in Staphylococcus aureus Biofilm-Associated Foreign-Body Infections |
| title_full | Relevant Role of Fibronectin-Binding Proteins in Staphylococcus aureus Biofilm-Associated Foreign-Body Infections |
| title_fullStr | Relevant Role of Fibronectin-Binding Proteins in Staphylococcus aureus Biofilm-Associated Foreign-Body Infections |
| title_full_unstemmed | Relevant Role of Fibronectin-Binding Proteins in Staphylococcus aureus Biofilm-Associated Foreign-Body Infections |
| title_short | Relevant Role of Fibronectin-Binding Proteins in Staphylococcus aureus Biofilm-Associated Foreign-Body Infections |
| title_sort | relevant role of fibronectin binding proteins in staphylococcus aureus biofilm associated foreign body infections |
| url | http://hdl.handle.net/20.500.11939/4701 https://iai.asm.org/content/iai/77/9/3978.full.pdf |
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