Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm
Shoot tips and leafy bud fragments removed from offshoots of adult date palms (Phoenix dactylifera L.) were cultured on a nutrient medium containing the Murashige and Skoog inorganic salts, 453 mu M 2,4-dichlorophenoxyacetic acid, 14.8 mu M N-6-(2-isopentenyl)adenine and 3 g l(-1) activated charcoal...
| Autores principales: | , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4697 |
| _version_ | 1855491799016013824 |
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| author | Veramendi, J. Navarro, Luis |
| author_browse | Navarro, Luis Veramendi, J. |
| author_facet | Veramendi, J. Navarro, Luis |
| author_sort | Veramendi, J. |
| collection | ReDivia |
| description | Shoot tips and leafy bud fragments removed from offshoots of adult date palms (Phoenix dactylifera L.) were cultured on a nutrient medium containing the Murashige and Skoog inorganic salts, 453 mu M 2,4-dichlorophenoxyacetic acid, 14.8 mu M N-6-(2-isopentenyl)adenine and 3 g l(-1) activated charcoal to develop nodular callus after 8 months of culture. Callus was cultured in agar-solidified and stationary or shaken liquid media containing half-strength MS inorganic salts, 3 g l(-1) activated charcoal and different sucrose concentrations to study the influence of these factors on somatic embryogenesis. The best conditions for embryo development were culturing in liquid medium shaken at 100 rpm for a period of 2 weeks without sucrose, followed by culture on 3% sucrose. |
| format | Artículo |
| id | ReDivia4697 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia46972025-04-25T14:44:11Z Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm Veramendi, J. Navarro, Luis Shoot tips and leafy bud fragments removed from offshoots of adult date palms (Phoenix dactylifera L.) were cultured on a nutrient medium containing the Murashige and Skoog inorganic salts, 453 mu M 2,4-dichlorophenoxyacetic acid, 14.8 mu M N-6-(2-isopentenyl)adenine and 3 g l(-1) activated charcoal to develop nodular callus after 8 months of culture. Callus was cultured in agar-solidified and stationary or shaken liquid media containing half-strength MS inorganic salts, 3 g l(-1) activated charcoal and different sucrose concentrations to study the influence of these factors on somatic embryogenesis. The best conditions for embryo development were culturing in liquid medium shaken at 100 rpm for a period of 2 weeks without sucrose, followed by culture on 3% sucrose. 2017-06-01T10:10:46Z 2017-06-01T10:10:46Z 1996 MAY article Veramendi, J., Navarro, L. (1996). Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm. Plant Cell Tissue and Organ Culture, 45(2), 159-164. 0167-6857 http://hdl.handle.net/20.500.11939/4697 10.1007/BF00048760 en openAccess Impreso |
| spellingShingle | Veramendi, J. Navarro, Luis Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm |
| title | Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm |
| title_full | Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm |
| title_fullStr | Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm |
| title_full_unstemmed | Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm |
| title_short | Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm |
| title_sort | influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm |
| url | http://hdl.handle.net/20.500.11939/4697 |
| work_keys_str_mv | AT veramendij influenceofphysicalconditionsofnutrientmediumandsucroseonsomaticembryogenesisofdatepalm AT navarroluis influenceofphysicalconditionsofnutrientmediumandsucroseonsomaticembryogenesisofdatepalm |