Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region
Sharka disease, caused by the Plum pox virus (PPV), is one of the main limiting factors for stone fruit crops worldwide. Only a few resistance sources have been found in apricot (Prunus armeniaca L.), and most studies have located a major PPV resistance locus (PPVres) on linkage group 1 (LG1). Howev...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4693 |
| _version_ | 1855491798462365696 |
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| author | Vera-Ruiz, Elsa M. Soriano, J. M. Romero, Carlos Zhebentyayeva, Tetyana Terol, Javier Zuriaga, Elena Llácer, Gerardo Abbott, Albert Glenn Badenes, María L. |
| author_browse | Abbott, Albert Glenn Badenes, María L. Llácer, Gerardo Romero, Carlos Soriano, J. M. Terol, Javier Vera-Ruiz, Elsa M. Zhebentyayeva, Tetyana Zuriaga, Elena |
| author_facet | Vera-Ruiz, Elsa M. Soriano, J. M. Romero, Carlos Zhebentyayeva, Tetyana Terol, Javier Zuriaga, Elena Llácer, Gerardo Abbott, Albert Glenn Badenes, María L. |
| author_sort | Vera-Ruiz, Elsa M. |
| collection | ReDivia |
| description | Sharka disease, caused by the Plum pox virus (PPV), is one of the main limiting factors for stone fruit crops worldwide. Only a few resistance sources have been found in apricot (Prunus armeniaca L.), and most studies have located a major PPV resistance locus (PPVres) on linkage group 1 (LG1). However, the mapping accuracy was not sufficiently reliable and PPVres was predicted within a low confidence interval. In this study, we have constructed two high-density simple sequence repeat (SSR) improved maps with 0.70 and 0.68 markers/cm, corresponding to LG1 of ‘Lito’ and ‘Goldrich’ PPV-resistant cultivars, respectively. Using these maps, and excluding genotype–phenotype incongruent individuals, a new binary trait locus (BTL) analysis for PPV resistance was performed, narrowing down the PPVres support intervals to 7.3 and 5.9 cm in ‘Lito’ and ‘Goldrich’, respectively. Subsequently, 71 overlapping oligonucleotides (overgo) probes were hybridized against an apricot bacterial artificial chromosome (BAC) library, identifying 870 single BACs from which 340 were anchored onto a map region of approximately 30–40cm encompassing PPVres. Partial BAC contigs assigned to the two allelic haplotypes (resistant/susceptible) of the PPVres locus were built by high-information content fingerprinting (HICF). In addition, a total of 300 BAC-derived sequences were obtained, and 257 showed significant homology with the peach genome scaffold_1 corresponding to LG1. According to the peach syntenic genome sequence, PPVres was predicted within a region of 2.16 Mb in which a few candidate resistance genes were identified. |
| format | Artículo |
| id | ReDivia4693 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia46932025-04-25T14:44:10Z Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region Vera-Ruiz, Elsa M. Soriano, J. M. Romero, Carlos Zhebentyayeva, Tetyana Terol, Javier Zuriaga, Elena Llácer, Gerardo Abbott, Albert Glenn Badenes, María L. Sharka disease, caused by the Plum pox virus (PPV), is one of the main limiting factors for stone fruit crops worldwide. Only a few resistance sources have been found in apricot (Prunus armeniaca L.), and most studies have located a major PPV resistance locus (PPVres) on linkage group 1 (LG1). However, the mapping accuracy was not sufficiently reliable and PPVres was predicted within a low confidence interval. In this study, we have constructed two high-density simple sequence repeat (SSR) improved maps with 0.70 and 0.68 markers/cm, corresponding to LG1 of ‘Lito’ and ‘Goldrich’ PPV-resistant cultivars, respectively. Using these maps, and excluding genotype–phenotype incongruent individuals, a new binary trait locus (BTL) analysis for PPV resistance was performed, narrowing down the PPVres support intervals to 7.3 and 5.9 cm in ‘Lito’ and ‘Goldrich’, respectively. Subsequently, 71 overlapping oligonucleotides (overgo) probes were hybridized against an apricot bacterial artificial chromosome (BAC) library, identifying 870 single BACs from which 340 were anchored onto a map region of approximately 30–40cm encompassing PPVres. Partial BAC contigs assigned to the two allelic haplotypes (resistant/susceptible) of the PPVres locus were built by high-information content fingerprinting (HICF). In addition, a total of 300 BAC-derived sequences were obtained, and 257 showed significant homology with the peach genome scaffold_1 corresponding to LG1. According to the peach syntenic genome sequence, PPVres was predicted within a region of 2.16 Mb in which a few candidate resistance genes were identified. 2017-06-01T10:10:46Z 2017-06-01T10:10:46Z 2011 AUG 2011 article publishedVersion Vera-Ruiz, E. M., Soriano, J. M., Romero, C., Zhebentyayeva, T., Terol, J., Zuriaga, E. et al. (2011). Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region. Molecular Plant Pathology, 12(6), 535-547. 1464-6722 http://hdl.handle.net/20.500.11939/4693 10.1111/J.1364-3703.2010.00691.X en openAccess Impreso |
| spellingShingle | Vera-Ruiz, Elsa M. Soriano, J. M. Romero, Carlos Zhebentyayeva, Tetyana Terol, Javier Zuriaga, Elena Llácer, Gerardo Abbott, Albert Glenn Badenes, María L. Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region |
| title | Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region |
| title_full | Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region |
| title_fullStr | Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region |
| title_full_unstemmed | Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region |
| title_short | Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region |
| title_sort | narrowing down the apricot plum pox virus resistance locus and comparative analysis with the peach genome syntenic region |
| url | http://hdl.handle.net/20.500.11939/4693 |
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