Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality
Background: During freezing the selective precipitation of substances in the medium may provoke a pH shift and lead to sperm damage. Objective: To study the effect of the pH preadjustment in the freezing extender on post-thaw boar sperm quality. Methods: A total of 15 ejaculates from different boa...
| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4596 |
| _version_ | 1855032147192053760 |
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| author | Tomás, Cristina Gómez-Fernández, José Gómez-Izquierdo, Emilio Gómez, Ernesto A. Sanchez-Sanchez, Raul Gonzalez-Bulnes, Antonio De-Mercado, Eduardo |
| author_browse | De-Mercado, Eduardo Gonzalez-Bulnes, Antonio Gómez, Ernesto A. Gómez-Fernández, José Gómez-Izquierdo, Emilio Sanchez-Sanchez, Raul Tomás, Cristina |
| author_facet | Tomás, Cristina Gómez-Fernández, José Gómez-Izquierdo, Emilio Gómez, Ernesto A. Sanchez-Sanchez, Raul Gonzalez-Bulnes, Antonio De-Mercado, Eduardo |
| author_sort | Tomás, Cristina |
| collection | ReDivia |
| description | Background: During freezing the selective precipitation of substances in the medium may
provoke a pH shift and lead to sperm damage. Objective: To study the effect of the pH preadjustment in the freezing extender on post-thaw boar sperm quality. Methods: A total of 15
ejaculates from different boars were obtained and divided into six aliquots prior to a standard straw
cryopreservation in freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) with different pH.
After thawing, sperm quality (plasma membrane integrity, motility and acrosome status) were assessed
at 30 and 90 minutes of post-thaw incubation at 37ºC.
Results: When the boar sperm were frozen
in a freezing media with pH basic, and particularly at pH 8, it had higher post-thaw sperm quality.
Conclusion: The pre-adjustment at pH 8 of the freezing extender (lactose-egg yolk-glycerol-Orvus
ES Paste) is able to improve the post-thaw boar sperm quality. |
| format | article |
| id | ReDivia4596 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia45962025-04-25T14:43:57Z Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality Tomás, Cristina Gómez-Fernández, José Gómez-Izquierdo, Emilio Gómez, Ernesto A. Sanchez-Sanchez, Raul Gonzalez-Bulnes, Antonio De-Mercado, Eduardo Background: During freezing the selective precipitation of substances in the medium may provoke a pH shift and lead to sperm damage. Objective: To study the effect of the pH preadjustment in the freezing extender on post-thaw boar sperm quality. Methods: A total of 15 ejaculates from different boars were obtained and divided into six aliquots prior to a standard straw cryopreservation in freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) with different pH. After thawing, sperm quality (plasma membrane integrity, motility and acrosome status) were assessed at 30 and 90 minutes of post-thaw incubation at 37ºC. Results: When the boar sperm were frozen in a freezing media with pH basic, and particularly at pH 8, it had higher post-thaw sperm quality. Conclusion: The pre-adjustment at pH 8 of the freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) is able to improve the post-thaw boar sperm quality. 2017-06-01T10:10:30Z 2017-06-01T10:10:30Z 2015 MAR-APR 2015 article Tomás, C., Gómez-Fernández, J., Gómez-Izquierdo, E., Gómez-Fidalgo, E., Sánchez-Sánchez, R., González-Bulnes, A., & de Mercado, E. (2015). Effect of the ph pre-adjustment in the freezing extender on post-thaw boar sperm quality. Cryoletters, 36(2), 97-103. 0143-2044 http://hdl.handle.net/20.500.11939/4596 en openAccess Impreso |
| spellingShingle | Tomás, Cristina Gómez-Fernández, José Gómez-Izquierdo, Emilio Gómez, Ernesto A. Sanchez-Sanchez, Raul Gonzalez-Bulnes, Antonio De-Mercado, Eduardo Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality |
| title | Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality |
| title_full | Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality |
| title_fullStr | Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality |
| title_full_unstemmed | Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality |
| title_short | Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality |
| title_sort | effect of the ph pre adjustment in the freezing extender on post thaw boar sperm quality |
| url | http://hdl.handle.net/20.500.11939/4596 |
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