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Addition of Cholesterol-Loaded Cyclodextrins to the Thawing Extender: Effects on Boar Sperm Quality

The aim of the present study was to evaluate the effect that the addition of cholesterol‐loaded cyclodextrins (CLC) to the thawing extender has on the quality of frozen‐thawed boar sperm. Pooled semen (n = 5) from three boars was used for the experiments. The semen was cryopreserved with an egg‐yolk...

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Main Authors: Tomás, Cristina, Gómez-Fernández, José, Gómez-Izquierdo, Emilio, Mocé, Eva, De-Mercado, Eduardo
Format: article
Language:Inglés
Published: 2017
Online Access:http://hdl.handle.net/20.500.11939/4589
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author Tomás, Cristina
Gómez-Fernández, José
Gómez-Izquierdo, Emilio
Mocé, Eva
De-Mercado, Eduardo
author_browse De-Mercado, Eduardo
Gómez-Fernández, José
Gómez-Izquierdo, Emilio
Mocé, Eva
Tomás, Cristina
author_facet Tomás, Cristina
Gómez-Fernández, José
Gómez-Izquierdo, Emilio
Mocé, Eva
De-Mercado, Eduardo
author_sort Tomás, Cristina
collection ReDivia
description The aim of the present study was to evaluate the effect that the addition of cholesterol‐loaded cyclodextrins (CLC) to the thawing extender has on the quality of frozen‐thawed boar sperm. Pooled semen (n = 5) from three boars was used for the experiments. The semen was cryopreserved with an egg‐yolk‐based extender, it was diluted after thawing in Beltsville thawing solution (BTS) supplemented with different concentrations of CLC (0, 12.5, 25, 50 or 100 mg/500 × 106 sperm), and these samples were incubated at 37°C for 150 min. The following parameters of sperm quality were evaluated 30 and 150 min after incubation: sperm with intact plasma membrane (SIPM; %), sperm with normal acrosomal ridge (NAR; %), total motile sperm (TMS; %), progressively motile sperm (PMS; %) and kinetic parameters. Both SIPM and NAR increased (p < 0.05) when the thawing extender was supplemented with 12.5, 25 and 50 mg CLC/500 × 106 sperm. Nevertheless, motility decreased (p < 0.05) when the concentration of CLC exceeded 12.5 mg CLC/500 × 106 sperm. In conclusion, our results suggest that the supplementation of thawing extenders with CLC improves sperm viability and reduces acrosome damage after freezing/thawing.
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institution Instituto Valenciano de Investigaciones Agrarias (IVIA)
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spelling ReDivia45892025-04-25T14:43:56Z Addition of Cholesterol-Loaded Cyclodextrins to the Thawing Extender: Effects on Boar Sperm Quality Tomás, Cristina Gómez-Fernández, José Gómez-Izquierdo, Emilio Mocé, Eva De-Mercado, Eduardo The aim of the present study was to evaluate the effect that the addition of cholesterol‐loaded cyclodextrins (CLC) to the thawing extender has on the quality of frozen‐thawed boar sperm. Pooled semen (n = 5) from three boars was used for the experiments. The semen was cryopreserved with an egg‐yolk‐based extender, it was diluted after thawing in Beltsville thawing solution (BTS) supplemented with different concentrations of CLC (0, 12.5, 25, 50 or 100 mg/500 × 106 sperm), and these samples were incubated at 37°C for 150 min. The following parameters of sperm quality were evaluated 30 and 150 min after incubation: sperm with intact plasma membrane (SIPM; %), sperm with normal acrosomal ridge (NAR; %), total motile sperm (TMS; %), progressively motile sperm (PMS; %) and kinetic parameters. Both SIPM and NAR increased (p < 0.05) when the thawing extender was supplemented with 12.5, 25 and 50 mg CLC/500 × 106 sperm. Nevertheless, motility decreased (p < 0.05) when the concentration of CLC exceeded 12.5 mg CLC/500 × 106 sperm. In conclusion, our results suggest that the supplementation of thawing extenders with CLC improves sperm viability and reduces acrosome damage after freezing/thawing. 2017-06-01T10:10:29Z 2017-06-01T10:10:29Z 2014 JUN 2014 article Tomas, C., Gomez-Fernandez, J., Gomez-Izquierdo, E., Moce, E. & de-Mercado, E. (2014). Addition of CholE.ol-Loaded Cyclodextrins to the Thawing Extender: Effects on Boar Sperm Quality. Reproduction in Domestic Animals, 49(3), 427-432. 0936-6768 http://hdl.handle.net/20.500.11939/4589 10.1111/rda.12291 en Info:eu-repo/grantAgreement/ESF//RYC-2010-06162 This research was supported by project 2010-1282 Plan de Investigación del ITACyL. E. Mocé was supported by funds from Ministerio deCiencia e Innovación from Subprograma Ramón y Cajal co-financedby the European Social Fund (Ref. RYC-2010-06162; Madrid, Spain). openAccess Impreso
spellingShingle Tomás, Cristina
Gómez-Fernández, José
Gómez-Izquierdo, Emilio
Mocé, Eva
De-Mercado, Eduardo
Addition of Cholesterol-Loaded Cyclodextrins to the Thawing Extender: Effects on Boar Sperm Quality
title Addition of Cholesterol-Loaded Cyclodextrins to the Thawing Extender: Effects on Boar Sperm Quality
title_full Addition of Cholesterol-Loaded Cyclodextrins to the Thawing Extender: Effects on Boar Sperm Quality
title_fullStr Addition of Cholesterol-Loaded Cyclodextrins to the Thawing Extender: Effects on Boar Sperm Quality
title_full_unstemmed Addition of Cholesterol-Loaded Cyclodextrins to the Thawing Extender: Effects on Boar Sperm Quality
title_short Addition of Cholesterol-Loaded Cyclodextrins to the Thawing Extender: Effects on Boar Sperm Quality
title_sort addition of cholesterol loaded cyclodextrins to the thawing extender effects on boar sperm quality
url http://hdl.handle.net/20.500.11939/4589
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AT gomezizquierdoemilio additionofcholesterolloadedcyclodextrinstothethawingextendereffectsonboarspermquality
AT moceeva additionofcholesterolloadedcyclodextrinstothethawingextendereffectsonboarspermquality
AT demercadoeduardo additionofcholesterolloadedcyclodextrinstothethawingextendereffectsonboarspermquality

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