Fully "recombinant enzyme-linked immunosorbent assays" using genetically engineered single-chain antibody fusion proteins for detection of Citrus tristeza virus
Recombinant single-chain variable fragment antibodies (scFv) that bind specifically to Citrus tristeza virus (CTV), which cause the most detrimental viral disease in the citrus industry worldwide, were obtained from the hybridoma cell lines 3DF1 and 3CA5. These scFv were genetically fused with dimer...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/4582 |
| _version_ | 1855491780304175104 |
|---|---|
| author | Terrada, E. Kerschbaumer, R. J. Giunta, Giuseppe Galeffi, P. Himmler, G. Cambra, Mariano |
| author_browse | Cambra, Mariano Galeffi, P. Giunta, Giuseppe Himmler, G. Kerschbaumer, R. J. Terrada, E. |
| author_facet | Terrada, E. Kerschbaumer, R. J. Giunta, Giuseppe Galeffi, P. Himmler, G. Cambra, Mariano |
| author_sort | Terrada, E. |
| collection | ReDivia |
| description | Recombinant single-chain variable fragment antibodies (scFv) that bind specifically to Citrus tristeza virus (CTV), which cause the most detrimental viral disease in the citrus industry worldwide, were obtained from the hybridoma cell lines 3DF1 and 3CA5. These scFv were genetically fused with dimerization domains as well as with alkaline phosphatase, respectively, and diagnostic reagents were produced by expressing these fusion proteins in bacterial cultures. The engineered antibodies were successfully used for CTV diagnosis in plants by tissue print enzyme-linked immunosorbent assay (ELISA) and double antibody sandwich-ELISA. The fully recombinant ELISAs were as specific and sensitive as conventional ELISAs performed with the parental monoclonal and bodies, showing the usefulness of recombinant antibodies for routine detection of a virus in woody plants for the first time. |
| format | Artículo |
| id | ReDivia4582 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia45822025-04-25T14:43:55Z Fully "recombinant enzyme-linked immunosorbent assays" using genetically engineered single-chain antibody fusion proteins for detection of Citrus tristeza virus Terrada, E. Kerschbaumer, R. J. Giunta, Giuseppe Galeffi, P. Himmler, G. Cambra, Mariano Recombinant single-chain variable fragment antibodies (scFv) that bind specifically to Citrus tristeza virus (CTV), which cause the most detrimental viral disease in the citrus industry worldwide, were obtained from the hybridoma cell lines 3DF1 and 3CA5. These scFv were genetically fused with dimerization domains as well as with alkaline phosphatase, respectively, and diagnostic reagents were produced by expressing these fusion proteins in bacterial cultures. The engineered antibodies were successfully used for CTV diagnosis in plants by tissue print enzyme-linked immunosorbent assay (ELISA) and double antibody sandwich-ELISA. The fully recombinant ELISAs were as specific and sensitive as conventional ELISAs performed with the parental monoclonal and bodies, showing the usefulness of recombinant antibodies for routine detection of a virus in woody plants for the first time. 2017-06-01T10:10:28Z 2017-06-01T10:10:28Z 2000 DEC 2000 article Terrada, E., Kerschbaumer, R. J., Giunta, G., Galeffi, P., Himmler, G., Cambra, M. (2000). Fully "recombinant enzyme-linked immunosorbent assays" using genetically engineered single-chain antibody fusion proteins for detection of Citrus tristeza virus. Phytopathology, 90(12), 1337-1344. 0031-949X http://hdl.handle.net/20.500.11939/4582 10.1094/PHYTO.2000.90.12.1337 en openAccess Impreso |
| spellingShingle | Terrada, E. Kerschbaumer, R. J. Giunta, Giuseppe Galeffi, P. Himmler, G. Cambra, Mariano Fully "recombinant enzyme-linked immunosorbent assays" using genetically engineered single-chain antibody fusion proteins for detection of Citrus tristeza virus |
| title | Fully "recombinant enzyme-linked immunosorbent assays" using genetically engineered single-chain antibody fusion proteins for detection of Citrus tristeza virus |
| title_full | Fully "recombinant enzyme-linked immunosorbent assays" using genetically engineered single-chain antibody fusion proteins for detection of Citrus tristeza virus |
| title_fullStr | Fully "recombinant enzyme-linked immunosorbent assays" using genetically engineered single-chain antibody fusion proteins for detection of Citrus tristeza virus |
| title_full_unstemmed | Fully "recombinant enzyme-linked immunosorbent assays" using genetically engineered single-chain antibody fusion proteins for detection of Citrus tristeza virus |
| title_short | Fully "recombinant enzyme-linked immunosorbent assays" using genetically engineered single-chain antibody fusion proteins for detection of Citrus tristeza virus |
| title_sort | fully recombinant enzyme linked immunosorbent assays using genetically engineered single chain antibody fusion proteins for detection of citrus tristeza virus |
| url | http://hdl.handle.net/20.500.11939/4582 |
| work_keys_str_mv | AT terradae fullyrecombinantenzymelinkedimmunosorbentassaysusinggeneticallyengineeredsinglechainantibodyfusionproteinsfordetectionofcitrustristezavirus AT kerschbaumerrj fullyrecombinantenzymelinkedimmunosorbentassaysusinggeneticallyengineeredsinglechainantibodyfusionproteinsfordetectionofcitrustristezavirus AT giuntagiuseppe fullyrecombinantenzymelinkedimmunosorbentassaysusinggeneticallyengineeredsinglechainantibodyfusionproteinsfordetectionofcitrustristezavirus AT galeffip fullyrecombinantenzymelinkedimmunosorbentassaysusinggeneticallyengineeredsinglechainantibodyfusionproteinsfordetectionofcitrustristezavirus AT himmlerg fullyrecombinantenzymelinkedimmunosorbentassaysusinggeneticallyengineeredsinglechainantibodyfusionproteinsfordetectionofcitrustristezavirus AT cambramariano fullyrecombinantenzymelinkedimmunosorbentassaysusinggeneticallyengineeredsinglechainantibodyfusionproteinsfordetectionofcitrustristezavirus |