Involvement of a citrus meiotic recombination TTC-repeat motif in the formation of gross deletions generated by ionizing radiation and MULE activation
Background: Transposable-element mediated chromosomal rearrangements require the involvement of two transposons and two double-strand breaks (DSB) located in close proximity. In radiobiology, DSB proximity is also a major factor contributing to rearrangements. However, the whole issue of DSB proximi...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4579 |
| _version_ | 1855491779710681088 |
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| author | Terol, Javier Ibanez, Victoria Carbonell, Jose Alonso, Roberto Estornell, Leandro H. Licciardello, Concetta Gut, Ivo G. Dopazo, Joaquín Talón, Manuel |
| author_browse | Alonso, Roberto Carbonell, Jose Dopazo, Joaquín Estornell, Leandro H. Gut, Ivo G. Ibanez, Victoria Licciardello, Concetta Talón, Manuel Terol, Javier |
| author_facet | Terol, Javier Ibanez, Victoria Carbonell, Jose Alonso, Roberto Estornell, Leandro H. Licciardello, Concetta Gut, Ivo G. Dopazo, Joaquín Talón, Manuel |
| author_sort | Terol, Javier |
| collection | ReDivia |
| description | Background: Transposable-element mediated chromosomal rearrangements require the involvement of two transposons and two double-strand breaks (DSB) located in close proximity. In radiobiology, DSB proximity is also a major factor contributing to rearrangements. However, the whole issue of DSB proximity remains virtually unexplored. Results: Based on DNA sequencing analysis we show that the genomes of 2 derived mutations, Arrufatina (sport) and Nero (irradiation), share a similar 2 Mb deletion of chromosome 3. A 7 kb Mutator like element found in Clemenules was present in Arrufatina in inverted orientation flanking the 5' end of the deletion. The Arrufatina Mule displayed "dissimilar" 9-bp target site duplications separated by 2 Mb. Fine-scale single nucleotide variant analyses of the deleted fragments identified a TTC-repeat sequence motif located in the center of the deletion responsible of a meiotic crossover detected in the citrus reference genome. Conclusions: Taken together, this information is compatible with the proposal that in both mutants, the TTC-repeat motif formed a triplex DNA structure generating a loop that brought in close proximity the originally distinct reactive ends. In Arrufatina, the loop brought the Mule ends nearby the 2 distinct insertion target sites and the inverted insertion of the transposable element between these target sites provoked the release of the in-between fragment. This proposal requires the involvement of a unique transposon and sheds light on the unresolved question of how two distinct sites become located in close proximity. These observations confer a crucial role to the TTC-repeats in fundamental plant processes as meiotic recombination and chromosomal rearrangements. |
| format | Artículo |
| id | ReDivia4579 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia45792025-04-25T14:43:54Z Involvement of a citrus meiotic recombination TTC-repeat motif in the formation of gross deletions generated by ionizing radiation and MULE activation Terol, Javier Ibanez, Victoria Carbonell, Jose Alonso, Roberto Estornell, Leandro H. Licciardello, Concetta Gut, Ivo G. Dopazo, Joaquín Talón, Manuel Background: Transposable-element mediated chromosomal rearrangements require the involvement of two transposons and two double-strand breaks (DSB) located in close proximity. In radiobiology, DSB proximity is also a major factor contributing to rearrangements. However, the whole issue of DSB proximity remains virtually unexplored. Results: Based on DNA sequencing analysis we show that the genomes of 2 derived mutations, Arrufatina (sport) and Nero (irradiation), share a similar 2 Mb deletion of chromosome 3. A 7 kb Mutator like element found in Clemenules was present in Arrufatina in inverted orientation flanking the 5' end of the deletion. The Arrufatina Mule displayed "dissimilar" 9-bp target site duplications separated by 2 Mb. Fine-scale single nucleotide variant analyses of the deleted fragments identified a TTC-repeat sequence motif located in the center of the deletion responsible of a meiotic crossover detected in the citrus reference genome. Conclusions: Taken together, this information is compatible with the proposal that in both mutants, the TTC-repeat motif formed a triplex DNA structure generating a loop that brought in close proximity the originally distinct reactive ends. In Arrufatina, the loop brought the Mule ends nearby the 2 distinct insertion target sites and the inverted insertion of the transposable element between these target sites provoked the release of the in-between fragment. This proposal requires the involvement of a unique transposon and sheds light on the unresolved question of how two distinct sites become located in close proximity. These observations confer a crucial role to the TTC-repeats in fundamental plant processes as meiotic recombination and chromosomal rearrangements. 2017-06-01T10:10:28Z 2017-06-01T10:10:28Z 2015 FEB 13 2015 article publishedVersion Terol, J., Ibanez, Victoria, Carbonell, J., Alonso, Roberto, Estornell, L. H., Licciardello, Concetta, Gut, Ivo G., Dopazo, Joaqun, Talon, M. (2015). Involvement of a citrus meiotic recombination TTC-repeat motif in the formation of gross deletions generated by ionizing radiation and MULE activation. Bmc Genomics, 16, 69-69. 1471-2164 http://hdl.handle.net/20.500.11939/4579 10.1186/s12864-015-1280-3 en openAccess Impreso |
| spellingShingle | Terol, Javier Ibanez, Victoria Carbonell, Jose Alonso, Roberto Estornell, Leandro H. Licciardello, Concetta Gut, Ivo G. Dopazo, Joaquín Talón, Manuel Involvement of a citrus meiotic recombination TTC-repeat motif in the formation of gross deletions generated by ionizing radiation and MULE activation |
| title | Involvement of a citrus meiotic recombination TTC-repeat motif in the formation of gross deletions generated by ionizing radiation and MULE activation |
| title_full | Involvement of a citrus meiotic recombination TTC-repeat motif in the formation of gross deletions generated by ionizing radiation and MULE activation |
| title_fullStr | Involvement of a citrus meiotic recombination TTC-repeat motif in the formation of gross deletions generated by ionizing radiation and MULE activation |
| title_full_unstemmed | Involvement of a citrus meiotic recombination TTC-repeat motif in the formation of gross deletions generated by ionizing radiation and MULE activation |
| title_short | Involvement of a citrus meiotic recombination TTC-repeat motif in the formation of gross deletions generated by ionizing radiation and MULE activation |
| title_sort | involvement of a citrus meiotic recombination ttc repeat motif in the formation of gross deletions generated by ionizing radiation and mule activation |
| url | http://hdl.handle.net/20.500.11939/4579 |
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