Association of 'Candidatus Liberibacter solanacearum' with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection
A new symptomatology was observed in celery (Apium graveolens) in Villena, Spain in 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with ‘Candidatus Liberibacter solanacearum’ and not with phytoplasmas. Samples from pl...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4573 |
| _version_ | 1855032143347974144 |
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| author | Teresani, Gabriela Bertolini, Edson Alfaro-Fernández, Ana Martínez, M. Carmen Ossamu Tanaka, Francisco Andre Kitajima, Elliot W. Roselló, Montserrat Sanjuan, Susana Carlos Ferrandiz, Juan López, María M. Cambra, Mariano Font-San-Ambrosio, Isabel |
| author_browse | Alfaro-Fernández, Ana Bertolini, Edson Cambra, Mariano Carlos Ferrandiz, Juan Font-San-Ambrosio, Isabel Kitajima, Elliot W. López, María M. Martínez, M. Carmen Ossamu Tanaka, Francisco Andre Roselló, Montserrat Sanjuan, Susana Teresani, Gabriela |
| author_facet | Teresani, Gabriela Bertolini, Edson Alfaro-Fernández, Ana Martínez, M. Carmen Ossamu Tanaka, Francisco Andre Kitajima, Elliot W. Roselló, Montserrat Sanjuan, Susana Carlos Ferrandiz, Juan López, María M. Cambra, Mariano Font-San-Ambrosio, Isabel |
| author_sort | Teresani, Gabriela |
| collection | ReDivia |
| description | A new symptomatology was observed in celery (Apium graveolens) in Villena, Spain in 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with ‘Candidatus Liberibacter solanacearum’ and not with phytoplasmas. Samples from plant sap were immobilized on membranes based on the spot procedure and tested using a newly developed real-time polymerase chain reaction assay to detect ‘Ca. L. solanacearum’. Then, a test kit was developed and validated by intralaboratory assays with an accuracy of 100%. Bacterial-like cells with typical morphology of ‘Ca. Liberibacter’ were observed using electron microscopy in celery plant tissues. A fifth haplotype of ‘Ca. L. solanacearum’, named E, was identified in celery and in carrot after analyzing partial sequences of 16S and 50S ribosomal RNA genes. From our results, celery (family Apiaceae) can be listed as a new natural host of this emerging bacterium. |
| format | article |
| id | ReDivia4573 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia45732025-04-25T14:43:53Z Association of 'Candidatus Liberibacter solanacearum' with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection Teresani, Gabriela Bertolini, Edson Alfaro-Fernández, Ana Martínez, M. Carmen Ossamu Tanaka, Francisco Andre Kitajima, Elliot W. Roselló, Montserrat Sanjuan, Susana Carlos Ferrandiz, Juan López, María M. Cambra, Mariano Font-San-Ambrosio, Isabel A new symptomatology was observed in celery (Apium graveolens) in Villena, Spain in 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with ‘Candidatus Liberibacter solanacearum’ and not with phytoplasmas. Samples from plant sap were immobilized on membranes based on the spot procedure and tested using a newly developed real-time polymerase chain reaction assay to detect ‘Ca. L. solanacearum’. Then, a test kit was developed and validated by intralaboratory assays with an accuracy of 100%. Bacterial-like cells with typical morphology of ‘Ca. Liberibacter’ were observed using electron microscopy in celery plant tissues. A fifth haplotype of ‘Ca. L. solanacearum’, named E, was identified in celery and in carrot after analyzing partial sequences of 16S and 50S ribosomal RNA genes. From our results, celery (family Apiaceae) can be listed as a new natural host of this emerging bacterium. 2017-06-01T10:10:27Z 2017-06-01T10:10:27Z 2014 AUG 2014 article Teresani, Gabriela R., Bertolini, E., Alfaro-Fernandez, Ana, Martinez, C., Ossamu Tanaka, F. Andre, Kitajima, Elliot W., Rosello, Montserrat, SanJ., S., C. Ferrandiz, J., Lopez, M.M., Cambra, M., I. Font, M. (2014). Association of 'Candidatus Liberibacter solanacearum' with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection. Phytopathology, 104(8), 804-811. 0031-949X http://hdl.handle.net/20.500.11939/4573 10.1094/PHYTO-07-13-0182-R en openAccess Impreso |
| spellingShingle | Teresani, Gabriela Bertolini, Edson Alfaro-Fernández, Ana Martínez, M. Carmen Ossamu Tanaka, Francisco Andre Kitajima, Elliot W. Roselló, Montserrat Sanjuan, Susana Carlos Ferrandiz, Juan López, María M. Cambra, Mariano Font-San-Ambrosio, Isabel Association of 'Candidatus Liberibacter solanacearum' with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection |
| title | Association of 'Candidatus Liberibacter solanacearum' with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection |
| title_full | Association of 'Candidatus Liberibacter solanacearum' with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection |
| title_fullStr | Association of 'Candidatus Liberibacter solanacearum' with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection |
| title_full_unstemmed | Association of 'Candidatus Liberibacter solanacearum' with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection |
| title_short | Association of 'Candidatus Liberibacter solanacearum' with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection |
| title_sort | association of candidatus liberibacter solanacearum with a vegetative disorder of celery in spain and development of a real time pcr method for its detection |
| url | http://hdl.handle.net/20.500.11939/4573 |
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