Lipopolysaccharides as Determinants of Serological Variability in Pseudomonas-Corrugata
The variation in biochemical and serological features of 128 isolates of Pseudomonas corrugata has been studied with 56 isolates from Spain and 72 isolates from other countries. Isolates were analyzed with common diagnostic tests and with the API50CHE system. Variability among isolates for some stan...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4526 |
| _version_ | 1855032135709097984 |
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| author | Siverio, Felipe Cambra, Mariano Gorris, María T. Corzo, J. López, María M. |
| author_browse | Cambra, Mariano Corzo, J. Gorris, María T. López, María M. Siverio, Felipe |
| author_facet | Siverio, Felipe Cambra, Mariano Gorris, María T. Corzo, J. López, María M. |
| author_sort | Siverio, Felipe |
| collection | ReDivia |
| description | The variation in biochemical and serological features of 128 isolates of Pseudomonas corrugata has been studied with 56 isolates from Spain and 72 isolates from other countries. Isolates were analyzed with common diagnostic tests and with the API50CHE system. Variability among isolates for some standard tests usually listed as positive or negative for this species, such as arginine dihydrolase and gelatin hydrolysis, lipase and lecithinase activities, pigment production, and wrinkled colony morphology, was observed. Three antisera were raised against the type strain and two Spanish isolates from tomato and pepper plants. Serological reactions were studied by indirect immunofluorescence and indirect enzyme-linked immunosorbent assay. Eighty-three isolates reacted with a single antiserum, 6 reacted with two antisera, and none reacted with three antisera. Thirty-nine isolates did not react with any of the three antisera. These results suggest that serology will not be a useful method for routine diagnosis of P. corrugata unless common antigens can be identified. Electrophoresis and immunoelectrotransfer were used to study the antigens involved. Each antiserum reacted with whole-cell lysates, giving two common hands for P. corrugata isolates and other Pseudomonas species and a ladder-like pattern characteristic of lipopolysaccharides (LPS). Common bands were not observed after proteinase K treatment. More than 10 LPS patterns were distinguished in 98 isolates after silver staining of polyacrylamide gels. There was no correlation between the geographical origin or host of the isolates and the LPS patterns. A correlation between LPS groups and serological reaction was observed. |
| format | article |
| id | ReDivia4526 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia45262025-04-25T14:43:46Z Lipopolysaccharides as Determinants of Serological Variability in Pseudomonas-Corrugata Siverio, Felipe Cambra, Mariano Gorris, María T. Corzo, J. López, María M. The variation in biochemical and serological features of 128 isolates of Pseudomonas corrugata has been studied with 56 isolates from Spain and 72 isolates from other countries. Isolates were analyzed with common diagnostic tests and with the API50CHE system. Variability among isolates for some standard tests usually listed as positive or negative for this species, such as arginine dihydrolase and gelatin hydrolysis, lipase and lecithinase activities, pigment production, and wrinkled colony morphology, was observed. Three antisera were raised against the type strain and two Spanish isolates from tomato and pepper plants. Serological reactions were studied by indirect immunofluorescence and indirect enzyme-linked immunosorbent assay. Eighty-three isolates reacted with a single antiserum, 6 reacted with two antisera, and none reacted with three antisera. Thirty-nine isolates did not react with any of the three antisera. These results suggest that serology will not be a useful method for routine diagnosis of P. corrugata unless common antigens can be identified. Electrophoresis and immunoelectrotransfer were used to study the antigens involved. Each antiserum reacted with whole-cell lysates, giving two common hands for P. corrugata isolates and other Pseudomonas species and a ladder-like pattern characteristic of lipopolysaccharides (LPS). Common bands were not observed after proteinase K treatment. More than 10 LPS patterns were distinguished in 98 isolates after silver staining of polyacrylamide gels. There was no correlation between the geographical origin or host of the isolates and the LPS patterns. A correlation between LPS groups and serological reaction was observed. 2017-06-01T10:10:21Z 2017-06-01T10:10:21Z 1993 JUN 1993 article Siverio, F., Cambra, M., Gorris, M. T., Corzo, J. & Lopez, M. M. (1993). Lipopolysaccharides as Determinants of Serological Variability in Pseudomonas-Corrugata. Applied and Environmental Microbiology, 59(6), 1805-1812. 0099-2240 http://hdl.handle.net/20.500.11939/4526 10.1128/aem.59.6.1805-1812.1993 en openAccess Impreso |
| spellingShingle | Siverio, Felipe Cambra, Mariano Gorris, María T. Corzo, J. López, María M. Lipopolysaccharides as Determinants of Serological Variability in Pseudomonas-Corrugata |
| title | Lipopolysaccharides as Determinants of Serological Variability in Pseudomonas-Corrugata |
| title_full | Lipopolysaccharides as Determinants of Serological Variability in Pseudomonas-Corrugata |
| title_fullStr | Lipopolysaccharides as Determinants of Serological Variability in Pseudomonas-Corrugata |
| title_full_unstemmed | Lipopolysaccharides as Determinants of Serological Variability in Pseudomonas-Corrugata |
| title_short | Lipopolysaccharides as Determinants of Serological Variability in Pseudomonas-Corrugata |
| title_sort | lipopolysaccharides as determinants of serological variability in pseudomonas corrugata |
| url | http://hdl.handle.net/20.500.11939/4526 |
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