Flanking the major Plum pox virus resistance locus in apricot with co-dominant markers (SSRs) derived from candidate resistance genes

Plum pox virus (PPV) is a potyvirus that causes sharka disease in infested stone fruit trees (Prunus species, peach, apricot, plum). In apricots, the resistance is controlled by a major quantitative trait locus that explains up to 70% of the phenotypic variance; it is localised in the upper part of...

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Autores principales: Sicard, O., Marandel, G., Soriano, J. M., Lalli, D. A., Lambert, P., Salava, J., Badenes, María L., Abbott, Albert Glenn, Decroocq, V.
Formato: Artículo
Lenguaje:Inglés
Publicado: 2017
Acceso en línea:http://hdl.handle.net/20.500.11939/4520
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author Sicard, O.
Marandel, G.
Soriano, J. M.
Lalli, D. A.
Lambert, P.
Salava, J.
Badenes, María L.
Abbott, Albert Glenn
Decroocq, V.
author_browse Abbott, Albert Glenn
Badenes, María L.
Decroocq, V.
Lalli, D. A.
Lambert, P.
Marandel, G.
Salava, J.
Sicard, O.
Soriano, J. M.
author_facet Sicard, O.
Marandel, G.
Soriano, J. M.
Lalli, D. A.
Lambert, P.
Salava, J.
Badenes, María L.
Abbott, Albert Glenn
Decroocq, V.
author_sort Sicard, O.
collection ReDivia
description Plum pox virus (PPV) is a potyvirus that causes sharka disease in infested stone fruit trees (Prunus species, peach, apricot, plum). In apricots, the resistance is controlled by a major quantitative trait locus that explains up to 70% of the phenotypic variance; it is localised in the upper part of linkage group 1. In this report, we transformed candidate genes that mapped in the region of the apricot resistance locus into polymerase chain reaction markers (SSCP and SSR) and tested for their co-localisation with the major PPV resistance locus in related and unrelated populations. Three populations of F1 and F2 individuals issued from crosses between the PPV-resistant cultivar ‘Stark Early Orange’ or ‘Goldrich’ and three susceptible parents were used in this study. Molecular-marker data were collected to determine the linkage relationship between the PPV resistance locus in apricots and markers that target candidate disease-resistance genes. In addition, SSR markers linked to resistance-gene candidates were mapped to positions flanking the PPV resistance locus in different apricot populations. Therefore, we demonstrate that this strategy helps to saturate the major genomic region controlling resistance to PPV in apricot with valuable co-dominant markers
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spelling ReDivia45202025-04-25T14:43:45Z Flanking the major Plum pox virus resistance locus in apricot with co-dominant markers (SSRs) derived from candidate resistance genes Sicard, O. Marandel, G. Soriano, J. M. Lalli, D. A. Lambert, P. Salava, J. Badenes, María L. Abbott, Albert Glenn Decroocq, V. Plum pox virus (PPV) is a potyvirus that causes sharka disease in infested stone fruit trees (Prunus species, peach, apricot, plum). In apricots, the resistance is controlled by a major quantitative trait locus that explains up to 70% of the phenotypic variance; it is localised in the upper part of linkage group 1. In this report, we transformed candidate genes that mapped in the region of the apricot resistance locus into polymerase chain reaction markers (SSCP and SSR) and tested for their co-localisation with the major PPV resistance locus in related and unrelated populations. Three populations of F1 and F2 individuals issued from crosses between the PPV-resistant cultivar ‘Stark Early Orange’ or ‘Goldrich’ and three susceptible parents were used in this study. Molecular-marker data were collected to determine the linkage relationship between the PPV resistance locus in apricots and markers that target candidate disease-resistance genes. In addition, SSR markers linked to resistance-gene candidates were mapped to positions flanking the PPV resistance locus in different apricot populations. Therefore, we demonstrate that this strategy helps to saturate the major genomic region controlling resistance to PPV in apricot with valuable co-dominant markers 2017-06-01T10:10:20Z 2017-06-01T10:10:20Z 2008 APR 2008 article Sicard, O., Marandel, G., Soriano, J. M., Lalli, D. A., Lambert, P., Salava, J. et al. (2008). Flanking the major Plum pox virus resistance locus in apricot with co-dominant markers (SSRs), derived from candidate resistance genes. Tree Genetics & Genomes, 4(2), 359-365. 1614-2942 http://hdl.handle.net/20.500.11939/4520 10.1007/s11295-007-0114-0 en openAccess Impreso
spellingShingle Sicard, O.
Marandel, G.
Soriano, J. M.
Lalli, D. A.
Lambert, P.
Salava, J.
Badenes, María L.
Abbott, Albert Glenn
Decroocq, V.
Flanking the major Plum pox virus resistance locus in apricot with co-dominant markers (SSRs) derived from candidate resistance genes
title Flanking the major Plum pox virus resistance locus in apricot with co-dominant markers (SSRs) derived from candidate resistance genes
title_full Flanking the major Plum pox virus resistance locus in apricot with co-dominant markers (SSRs) derived from candidate resistance genes
title_fullStr Flanking the major Plum pox virus resistance locus in apricot with co-dominant markers (SSRs) derived from candidate resistance genes
title_full_unstemmed Flanking the major Plum pox virus resistance locus in apricot with co-dominant markers (SSRs) derived from candidate resistance genes
title_short Flanking the major Plum pox virus resistance locus in apricot with co-dominant markers (SSRs) derived from candidate resistance genes
title_sort flanking the major plum pox virus resistance locus in apricot with co dominant markers ssrs derived from candidate resistance genes
url http://hdl.handle.net/20.500.11939/4520
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