Role for Rhizobium rhizogenes K84 Cell Envelope Polysaccharides in Surface Interactions
Rhizobium rhizogenes strain K84 is a commercial biocontrol agent used worldwide to control crown gall disease. The organism binds tightly to polypropylene substrate and efficiently colonizes root surfaces as complex, multilayered biofilms. A genetic screen identified two mutants in which these surfa...
| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4516 |
| _version_ | 1855032133816418304 |
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| author | Abarca-Grau, Ana M. Burbank, Lindsey P. de Paz, Hector D. Crespo-Rivas, Juan C. Marco-Noales, Ester López, María M. Vinardell, Jose M. von Bodman, Susanne B. Penyalver, Ramón |
| author_browse | Abarca-Grau, Ana M. Burbank, Lindsey P. Crespo-Rivas, Juan C. López, María M. Marco-Noales, Ester Penyalver, Ramón Vinardell, Jose M. de Paz, Hector D. von Bodman, Susanne B. |
| author_facet | Abarca-Grau, Ana M. Burbank, Lindsey P. de Paz, Hector D. Crespo-Rivas, Juan C. Marco-Noales, Ester López, María M. Vinardell, Jose M. von Bodman, Susanne B. Penyalver, Ramón |
| author_sort | Abarca-Grau, Ana M. |
| collection | ReDivia |
| description | Rhizobium rhizogenes strain K84 is a commercial biocontrol agent used worldwide to control crown gall disease. The organism binds tightly to polypropylene substrate and efficiently colonizes root surfaces as complex, multilayered biofilms. A genetic screen identified two mutants in which these surface interactions were affected. One of these mutants failed to attach and form biofilms on the abiotic surface although, interestingly, it exhibited normal biofilm formation on the biological root tip surface. This mutant is disrupted in a wcbD ortholog gene, which is part of a large locus predicted to encode functions for the biosynthesis and export of a group II capsular polysaccharide (CPS). Expression of a functional copy of wcbD in the mutant background restored the ability of the bacteria to attach and form normal biofilms on the abiotic surface. The second identified mutant attached and formed visibly denser biofilms on both abiotic and root tip surfaces. This mutant is disrupted in the rkpK gene, which is predicted to encode a UDP-glucose 6-dehydrogenase required for O-antigen lipopolysaccharide (LPS) and K-antigen capsular polysaccharide (KPS) biosynthesis in rhizobia. The rkpK mutant from strain K84 was deficient in O-antigen synthesis and exclusively produced rough LPS. We also show that strain K84 does not synthesize the KPS typical of some other rhizobia strains. In addition, we identified a putative type II CPS, distinct from KPS, that mediates cell-surface interactions, and we show that O antigen of strain K84 is necessary for normal cell-cell interactions in the biofilms. |
| format | article |
| id | ReDivia4516 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia45162025-04-25T14:43:45Z Role for Rhizobium rhizogenes K84 Cell Envelope Polysaccharides in Surface Interactions Abarca-Grau, Ana M. Burbank, Lindsey P. de Paz, Hector D. Crespo-Rivas, Juan C. Marco-Noales, Ester López, María M. Vinardell, Jose M. von Bodman, Susanne B. Penyalver, Ramón Rhizobium rhizogenes strain K84 is a commercial biocontrol agent used worldwide to control crown gall disease. The organism binds tightly to polypropylene substrate and efficiently colonizes root surfaces as complex, multilayered biofilms. A genetic screen identified two mutants in which these surface interactions were affected. One of these mutants failed to attach and form biofilms on the abiotic surface although, interestingly, it exhibited normal biofilm formation on the biological root tip surface. This mutant is disrupted in a wcbD ortholog gene, which is part of a large locus predicted to encode functions for the biosynthesis and export of a group II capsular polysaccharide (CPS). Expression of a functional copy of wcbD in the mutant background restored the ability of the bacteria to attach and form normal biofilms on the abiotic surface. The second identified mutant attached and formed visibly denser biofilms on both abiotic and root tip surfaces. This mutant is disrupted in the rkpK gene, which is predicted to encode a UDP-glucose 6-dehydrogenase required for O-antigen lipopolysaccharide (LPS) and K-antigen capsular polysaccharide (KPS) biosynthesis in rhizobia. The rkpK mutant from strain K84 was deficient in O-antigen synthesis and exclusively produced rough LPS. We also show that strain K84 does not synthesize the KPS typical of some other rhizobia strains. In addition, we identified a putative type II CPS, distinct from KPS, that mediates cell-surface interactions, and we show that O antigen of strain K84 is necessary for normal cell-cell interactions in the biofilms. 2017-06-01T10:10:19Z 2017-06-01T10:10:19Z 2012 MAR 2012 article Abarca-Grau, Ana M., Burbank, Lindsey P., de Paz, Hector D., Crespo-Rivas, J. C., Marco-Noales, E., Lopez, M.M., Vinardell, J.M., von Bodman, Susanne B., Penyalvera, R. (2012). Role for Rhizobium rhizogenes K84 Cell Envelope Polysaccharides in Surface Interactions. Applied and Environmental Microbiology, 78(6), 1644-1651. 0099-2240 http://hdl.handle.net/20.500.11939/4516 10.1128/AEM.07117-11 en openAccess Impreso |
| spellingShingle | Abarca-Grau, Ana M. Burbank, Lindsey P. de Paz, Hector D. Crespo-Rivas, Juan C. Marco-Noales, Ester López, María M. Vinardell, Jose M. von Bodman, Susanne B. Penyalver, Ramón Role for Rhizobium rhizogenes K84 Cell Envelope Polysaccharides in Surface Interactions |
| title | Role for Rhizobium rhizogenes K84 Cell Envelope Polysaccharides in Surface Interactions |
| title_full | Role for Rhizobium rhizogenes K84 Cell Envelope Polysaccharides in Surface Interactions |
| title_fullStr | Role for Rhizobium rhizogenes K84 Cell Envelope Polysaccharides in Surface Interactions |
| title_full_unstemmed | Role for Rhizobium rhizogenes K84 Cell Envelope Polysaccharides in Surface Interactions |
| title_short | Role for Rhizobium rhizogenes K84 Cell Envelope Polysaccharides in Surface Interactions |
| title_sort | role for rhizobium rhizogenes k84 cell envelope polysaccharides in surface interactions |
| url | http://hdl.handle.net/20.500.11939/4516 |
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