Development of a simplified NASBA protocol for detecting viable cells of the citrus pathogen Xanthomonas citri subsp citri under different treatments
Nucleic acid sequence based amplification (NASBA) is a method of amplifying RNA, for the detection of RNA viruses and human pathogenic bacteria. Recently, NASBA has also been employed for the detection of plant diseases caused by viruses and quarantine bacteria. A major citrus pathogen, Xanthomonas...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4506 https://bsppjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-3059.2010.02305.x |
| _version_ | 1855491766708338688 |
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| author | Scuderi, Giuseppe Golmohammadi, Morteza Cubero, Jaime López, María M. Cirvilleri, G. Llop, Pablo |
| author_browse | Cirvilleri, G. Cubero, Jaime Golmohammadi, Morteza Llop, Pablo López, María M. Scuderi, Giuseppe |
| author_facet | Scuderi, Giuseppe Golmohammadi, Morteza Cubero, Jaime López, María M. Cirvilleri, G. Llop, Pablo |
| author_sort | Scuderi, Giuseppe |
| collection | ReDivia |
| description | Nucleic acid sequence based amplification (NASBA) is a method of amplifying RNA, for the detection of RNA viruses and human pathogenic bacteria. Recently, NASBA has also been employed for the detection of plant diseases caused by viruses and quarantine bacteria. A major citrus pathogen, Xanthomonas citri subsp. citri (Xcc), causal agent of citrus bacterial canker, is being studied in depth due to its economic importance, with recent focus concentrating on its viability and survival under different stress conditions and control treatments. In this work, a NASBA protocol using primers for gumD mRNA has been developed to assess the viability of this pathogen under different bacteriocidal treatments. This method is rapid, specific and sensitive, and is able to detect viable bacterial cells, using a hybridization device which allows the visualization of the results in only 30 min. The usefulness of the method has been confirmed with bacterial suspensions subjected to different heat treatments and to sodium orthophenylphenate. |
| format | Artículo |
| id | ReDivia4506 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia45062025-04-25T14:43:44Z Development of a simplified NASBA protocol for detecting viable cells of the citrus pathogen Xanthomonas citri subsp citri under different treatments Scuderi, Giuseppe Golmohammadi, Morteza Cubero, Jaime López, María M. Cirvilleri, G. Llop, Pablo Nucleic acid sequence based amplification (NASBA) is a method of amplifying RNA, for the detection of RNA viruses and human pathogenic bacteria. Recently, NASBA has also been employed for the detection of plant diseases caused by viruses and quarantine bacteria. A major citrus pathogen, Xanthomonas citri subsp. citri (Xcc), causal agent of citrus bacterial canker, is being studied in depth due to its economic importance, with recent focus concentrating on its viability and survival under different stress conditions and control treatments. In this work, a NASBA protocol using primers for gumD mRNA has been developed to assess the viability of this pathogen under different bacteriocidal treatments. This method is rapid, specific and sensitive, and is able to detect viable bacterial cells, using a hybridization device which allows the visualization of the results in only 30 min. The usefulness of the method has been confirmed with bacterial suspensions subjected to different heat treatments and to sodium orthophenylphenate. 2017-06-01T10:10:18Z 2017-06-01T10:10:18Z 2010 AUG 2010 article publishedVersion Scuderi, G., Golmohammadi, M., Cubero, J., Lopez, M. M., Cirvilleri, G. & Llop, P. (2010). Development of a simplified NASBA protocol for detecting viable cells of the citrus pathogen Xanthomonas citri subsp citri under different treatments. Plant Pathology, 59(4), 764-772. 0032-0862 http://hdl.handle.net/20.500.11939/4506 10.1111/j.1365-3059.2010.02305.x https://bsppjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-3059.2010.02305.x en openAccess Impreso |
| spellingShingle | Scuderi, Giuseppe Golmohammadi, Morteza Cubero, Jaime López, María M. Cirvilleri, G. Llop, Pablo Development of a simplified NASBA protocol for detecting viable cells of the citrus pathogen Xanthomonas citri subsp citri under different treatments |
| title | Development of a simplified NASBA protocol for detecting viable cells of the citrus pathogen Xanthomonas citri subsp citri under different treatments |
| title_full | Development of a simplified NASBA protocol for detecting viable cells of the citrus pathogen Xanthomonas citri subsp citri under different treatments |
| title_fullStr | Development of a simplified NASBA protocol for detecting viable cells of the citrus pathogen Xanthomonas citri subsp citri under different treatments |
| title_full_unstemmed | Development of a simplified NASBA protocol for detecting viable cells of the citrus pathogen Xanthomonas citri subsp citri under different treatments |
| title_short | Development of a simplified NASBA protocol for detecting viable cells of the citrus pathogen Xanthomonas citri subsp citri under different treatments |
| title_sort | development of a simplified nasba protocol for detecting viable cells of the citrus pathogen xanthomonas citri subsp citri under different treatments |
| url | http://hdl.handle.net/20.500.11939/4506 https://bsppjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-3059.2010.02305.x |
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