Preparation of Additional Monoclonal-Antibodies for Detection and Discrimination of Potato Virus-Y Isolates Infecting Potato
Monoclonal antibodies (MAb) specific to potato virus Y (PVY) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate Bintje PVY(o). Six MAb were characterized. The reactions of the Mab have been assayed by indi...
| Main Authors: | , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/4500 |
| _version_ | 1855491765586362368 |
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| author | Sanz, Antonio Cambra, Mariano Desanroman, C. P. Miguet, J. G. Cortes, E. Gorris, María T. Vela, C. |
| author_browse | Cambra, Mariano Cortes, E. Desanroman, C. P. Gorris, María T. Miguet, J. G. Sanz, Antonio Vela, C. |
| author_facet | Sanz, Antonio Cambra, Mariano Desanroman, C. P. Miguet, J. G. Cortes, E. Gorris, María T. Vela, C. |
| author_sort | Sanz, Antonio |
| collection | ReDivia |
| description | Monoclonal antibodies (MAb) specific to potato virus Y (PVY) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate Bintje PVY(o). Six MAb were characterized. The reactions of the Mab have been assayed by indirect double-antibody sandwich enzyme-linked immunosorbent assay (ELISA-IDAS) against forty-nine PVY isolates and other potyviruses, representative of different potato areas in the world. All MAb reacted to at least some isolates in group O + C in ELISA-IDAS. One MAb did not react to any group N isolates. MAb 10E3 reacted to all isolates tested. Using standard double-antibody sandwich enzyme-linked immunosorbent assay (ELISA-DAS), two MAbs did not react to any isolates, one reacted to some isolates in group O + C and MAb 10E3 reacted to all isolates but two in group C. A mixture of MAb 10E3 and 1E10 detected all isolates in ELISA-DAS and sensitivity was improved over that obtained with polyclonal antibodies from antisera. |
| format | Artículo |
| id | ReDivia4500 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia45002025-04-25T14:43:42Z Preparation of Additional Monoclonal-Antibodies for Detection and Discrimination of Potato Virus-Y Isolates Infecting Potato Sanz, Antonio Cambra, Mariano Desanroman, C. P. Miguet, J. G. Cortes, E. Gorris, María T. Vela, C. Monoclonal antibodies (MAb) specific to potato virus Y (PVY) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate Bintje PVY(o). Six MAb were characterized. The reactions of the Mab have been assayed by indirect double-antibody sandwich enzyme-linked immunosorbent assay (ELISA-IDAS) against forty-nine PVY isolates and other potyviruses, representative of different potato areas in the world. All MAb reacted to at least some isolates in group O + C in ELISA-IDAS. One MAb did not react to any group N isolates. MAb 10E3 reacted to all isolates tested. Using standard double-antibody sandwich enzyme-linked immunosorbent assay (ELISA-DAS), two MAbs did not react to any isolates, one reacted to some isolates in group O + C and MAb 10E3 reacted to all isolates but two in group C. A mixture of MAb 10E3 and 1E10 detected all isolates in ELISA-DAS and sensitivity was improved over that obtained with polyclonal antibodies from antisera. 2017-06-01T10:10:17Z 2017-06-01T10:10:17Z 1990 SEP 1990 article Sanz, A., Cambra, M., Desanroman, C. P., Miguet, J. G., Cortes, E., Gorris, M. T., Vela, C. (1990). Preparation of Additional Monoclonal-Antibodies for Detection and Discrimination of Potato Virus-Y Isolates Infecting Potato. Potato Research, 33(3), 365-375. 0014-3065 http://hdl.handle.net/20.500.11939/4500 10.1007/BF02359311 en openAccess Impreso |
| spellingShingle | Sanz, Antonio Cambra, Mariano Desanroman, C. P. Miguet, J. G. Cortes, E. Gorris, María T. Vela, C. Preparation of Additional Monoclonal-Antibodies for Detection and Discrimination of Potato Virus-Y Isolates Infecting Potato |
| title | Preparation of Additional Monoclonal-Antibodies for Detection and Discrimination of Potato Virus-Y Isolates Infecting Potato |
| title_full | Preparation of Additional Monoclonal-Antibodies for Detection and Discrimination of Potato Virus-Y Isolates Infecting Potato |
| title_fullStr | Preparation of Additional Monoclonal-Antibodies for Detection and Discrimination of Potato Virus-Y Isolates Infecting Potato |
| title_full_unstemmed | Preparation of Additional Monoclonal-Antibodies for Detection and Discrimination of Potato Virus-Y Isolates Infecting Potato |
| title_short | Preparation of Additional Monoclonal-Antibodies for Detection and Discrimination of Potato Virus-Y Isolates Infecting Potato |
| title_sort | preparation of additional monoclonal antibodies for detection and discrimination of potato virus y isolates infecting potato |
| url | http://hdl.handle.net/20.500.11939/4500 |
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