The Genome of Cardinium cBtQ1 Provides Insights into Genome Reduction, Symbiont Motility, and Its Settlement in Bemisia tabaci
Many insects harbor inherited bacterial endosymbionts. Although some of them are not strictly essential and are considered facultative, they can be a key to host survival under specific environmental conditions, such as parasitoid attacks, climate changes, or insecticide pressures. The whitefly Bemi...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4499 https://academic.oup.com/gbe/article/6/4/1013/546926 |
| _version_ | 1855491765390278656 |
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| author | Santos-Garcia, Diego Rollat-Farnier, Pierre-Antoine Beitia, Francisco J. Zchori-Fein, Einat Vavre, Fabrice Mouton, Laurence Moya, Andres Latorre, Amparo Silva, Francisco J. |
| author_browse | Beitia, Francisco J. Latorre, Amparo Mouton, Laurence Moya, Andres Rollat-Farnier, Pierre-Antoine Santos-Garcia, Diego Silva, Francisco J. Vavre, Fabrice Zchori-Fein, Einat |
| author_facet | Santos-Garcia, Diego Rollat-Farnier, Pierre-Antoine Beitia, Francisco J. Zchori-Fein, Einat Vavre, Fabrice Mouton, Laurence Moya, Andres Latorre, Amparo Silva, Francisco J. |
| author_sort | Santos-Garcia, Diego |
| collection | ReDivia |
| description | Many insects harbor inherited bacterial endosymbionts. Although some of them are not strictly essential and are considered facultative, they can be a key to host survival under specific environmental conditions, such as parasitoid attacks, climate changes, or insecticide pressures. The whitefly Bemisia tabaci is at the top of the list of organisms inflicting agricultural damage and outbreaks, and changes in its distribution may be associated to global warming. In this work, we have sequenced and analyzed the genome of Cardinium cBtQ1, a facultative bacterial endosymbiont of B. tabaci and propose that it belongs to a new taxonomic family, which also includes Candidatus Amoebophilus asiaticus and Cardinium cEper1, endosymbionts of amoeba and wasps, respectively. Reconstruction of their last common ancestors’ gene contents revealed an initial massive gene loss from the free-living ancestor. This was followed in Cardinium by smaller losses, associated with settlement in arthropods. Some of these losses, affecting cofactor and amino acid biosynthetic encoding genes, took place in Cardinium cBtQ1 after its divergence from the Cardinium cEper1 lineage and were related to its settlement in the whitefly and its endosymbionts. Furthermore, the Cardinium cBtQ1 genome displays a large proportion of transposable elements, which have recently inactivated genes and produced chromosomal rearrangements. The genome also contains a chromosomal duplication and a multicopy plasmid, which harbors several genes putatively associated with gliding motility, as well as two other genes encoding proteins with potential insecticidal activity. As gene amplification is very rare in endosymbionts, an important function of these genes cannot be ruled out. |
| format | Artículo |
| id | ReDivia4499 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia44992025-04-25T14:43:42Z The Genome of Cardinium cBtQ1 Provides Insights into Genome Reduction, Symbiont Motility, and Its Settlement in Bemisia tabaci Santos-Garcia, Diego Rollat-Farnier, Pierre-Antoine Beitia, Francisco J. Zchori-Fein, Einat Vavre, Fabrice Mouton, Laurence Moya, Andres Latorre, Amparo Silva, Francisco J. Many insects harbor inherited bacterial endosymbionts. Although some of them are not strictly essential and are considered facultative, they can be a key to host survival under specific environmental conditions, such as parasitoid attacks, climate changes, or insecticide pressures. The whitefly Bemisia tabaci is at the top of the list of organisms inflicting agricultural damage and outbreaks, and changes in its distribution may be associated to global warming. In this work, we have sequenced and analyzed the genome of Cardinium cBtQ1, a facultative bacterial endosymbiont of B. tabaci and propose that it belongs to a new taxonomic family, which also includes Candidatus Amoebophilus asiaticus and Cardinium cEper1, endosymbionts of amoeba and wasps, respectively. Reconstruction of their last common ancestors’ gene contents revealed an initial massive gene loss from the free-living ancestor. This was followed in Cardinium by smaller losses, associated with settlement in arthropods. Some of these losses, affecting cofactor and amino acid biosynthetic encoding genes, took place in Cardinium cBtQ1 after its divergence from the Cardinium cEper1 lineage and were related to its settlement in the whitefly and its endosymbionts. Furthermore, the Cardinium cBtQ1 genome displays a large proportion of transposable elements, which have recently inactivated genes and produced chromosomal rearrangements. The genome also contains a chromosomal duplication and a multicopy plasmid, which harbors several genes putatively associated with gliding motility, as well as two other genes encoding proteins with potential insecticidal activity. As gene amplification is very rare in endosymbionts, an important function of these genes cannot be ruled out. 2017-06-01T10:10:17Z 2017-06-01T10:10:17Z 2014 2014 article publishedVersion Santos-Garcia, D., Rollat-Farnier, P. A., Beitia, F., Zchori-Fein, E., Vavre, F., Mouton, L. et al. (2014). The Genome of Cardinium cBtQ1 Provides Insights into Genome Reduction, Symbiont Motility, and Its Settlement in Bemisia tabaci. Genome Biology and Evolution, 6(4), 1013-1030. 1759-6653 http://hdl.handle.net/20.500.11939/4499 10.1093/gbe/evu077 https://academic.oup.com/gbe/article/6/4/1013/546926 en openAccess Impreso |
| spellingShingle | Santos-Garcia, Diego Rollat-Farnier, Pierre-Antoine Beitia, Francisco J. Zchori-Fein, Einat Vavre, Fabrice Mouton, Laurence Moya, Andres Latorre, Amparo Silva, Francisco J. The Genome of Cardinium cBtQ1 Provides Insights into Genome Reduction, Symbiont Motility, and Its Settlement in Bemisia tabaci |
| title | The Genome of Cardinium cBtQ1 Provides Insights into Genome Reduction, Symbiont Motility, and Its Settlement in Bemisia tabaci |
| title_full | The Genome of Cardinium cBtQ1 Provides Insights into Genome Reduction, Symbiont Motility, and Its Settlement in Bemisia tabaci |
| title_fullStr | The Genome of Cardinium cBtQ1 Provides Insights into Genome Reduction, Symbiont Motility, and Its Settlement in Bemisia tabaci |
| title_full_unstemmed | The Genome of Cardinium cBtQ1 Provides Insights into Genome Reduction, Symbiont Motility, and Its Settlement in Bemisia tabaci |
| title_short | The Genome of Cardinium cBtQ1 Provides Insights into Genome Reduction, Symbiont Motility, and Its Settlement in Bemisia tabaci |
| title_sort | genome of cardinium cbtq1 provides insights into genome reduction symbiont motility and its settlement in bemisia tabaci |
| url | http://hdl.handle.net/20.500.11939/4499 https://academic.oup.com/gbe/article/6/4/1013/546926 |
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