Effect of solid storage on caprine semen conservation at 5 degrees C
In this work, we investigated the effect of storage in solid-phase extender on buck semen conserved at 5 degrees C. Furthermore, we studied the effect of addition of cysteine to the extender and the washing of seminal plasma on sperm survival. In Experiment 1, milk-based extender (M) was used as a c...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4475 |
| _version_ | 1855032126975508480 |
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| author | Salvador, I. Yániz, Jesús L. Viudes-De-Castro, María P. Gómez, Ernesto A. Silvestre, Miguel A. |
| author_browse | Gómez, Ernesto A. Salvador, I. Silvestre, Miguel A. Viudes-De-Castro, María P. Yániz, Jesús L. |
| author_facet | Salvador, I. Yániz, Jesús L. Viudes-De-Castro, María P. Gómez, Ernesto A. Silvestre, Miguel A. |
| author_sort | Salvador, I. |
| collection | ReDivia |
| description | In this work, we investigated the effect of storage in solid-phase extender on buck semen conserved at 5 degrees C. Furthermore, we studied the effect of addition of cysteine to the extender and the washing of seminal plasma on sperm survival. In Experiment 1, milk-based extender (M) was used as a control to study the effect of solid media storage (G) and cysteine supplementation (C), and the combination of both (GC), on in vitro sperm survival of washed and non-washed semen, conserved up to 72 h at 5 degrees C. Motility, acrosome integrity (NAR) and hypo-osmotic swelling tests (HOST) were evaluated to assess in vitro sperm survival. In Experiment 2, an artificial insemination (AI) field trial was performed to compare G versus M. Solid media (G) maintained motility of spermatozoa during storage higher than any other extender (67% G versus 62% GC; 61% M and 59% C; P 0.05). No improvement in sperm viability was obtained by addition of cysteine to the media. Washing of semen improved motility (65% versus 60%; P 0.05). No improvement in sperm viability was obtained by addition of cysteine to the media. Washing of semen improved motility (65% versus 60%; P 0.05). No improvement in sperm viability was obtained by addition of cysteine to the media. Washing of semen improved motility (65% versus 60%; P 0.05). In conclusion, washing of semen and dilution in gelatin- supplemented milk extender (solid storage) appears to be a successful method for goat semen storage at 5 degrees C. (c) 2006 Elsevier Inc. All rights reserved. |
| format | article |
| id | ReDivia4475 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia44752025-04-25T14:43:38Z Effect of solid storage on caprine semen conservation at 5 degrees C Salvador, I. Yániz, Jesús L. Viudes-De-Castro, María P. Gómez, Ernesto A. Silvestre, Miguel A. In this work, we investigated the effect of storage in solid-phase extender on buck semen conserved at 5 degrees C. Furthermore, we studied the effect of addition of cysteine to the extender and the washing of seminal plasma on sperm survival. In Experiment 1, milk-based extender (M) was used as a control to study the effect of solid media storage (G) and cysteine supplementation (C), and the combination of both (GC), on in vitro sperm survival of washed and non-washed semen, conserved up to 72 h at 5 degrees C. Motility, acrosome integrity (NAR) and hypo-osmotic swelling tests (HOST) were evaluated to assess in vitro sperm survival. In Experiment 2, an artificial insemination (AI) field trial was performed to compare G versus M. Solid media (G) maintained motility of spermatozoa during storage higher than any other extender (67% G versus 62% GC; 61% M and 59% C; P 0.05). No improvement in sperm viability was obtained by addition of cysteine to the media. Washing of semen improved motility (65% versus 60%; P 0.05). No improvement in sperm viability was obtained by addition of cysteine to the media. Washing of semen improved motility (65% versus 60%; P 0.05). No improvement in sperm viability was obtained by addition of cysteine to the media. Washing of semen improved motility (65% versus 60%; P 0.05). In conclusion, washing of semen and dilution in gelatin- supplemented milk extender (solid storage) appears to be a successful method for goat semen storage at 5 degrees C. (c) 2006 Elsevier Inc. All rights reserved. 2017-06-01T10:10:13Z 2017-06-01T10:10:13Z 2006 SEP 1 2006 article Salvador, I., Yaniz, J., Viudes-de-Castro, M.P., Gomez, E.A., Silvestre, M.A. (2006). Effect of solid storage on caprine semen conservation at 5 degrees C. Theriogenology, 66(4), 974-981. 0093-691X http://hdl.handle.net/20.500.11939/4475 10.1016/j.theriogenology.2006.02.042 en openAccess Impreso |
| spellingShingle | Salvador, I. Yániz, Jesús L. Viudes-De-Castro, María P. Gómez, Ernesto A. Silvestre, Miguel A. Effect of solid storage on caprine semen conservation at 5 degrees C |
| title | Effect of solid storage on caprine semen conservation at 5 degrees C |
| title_full | Effect of solid storage on caprine semen conservation at 5 degrees C |
| title_fullStr | Effect of solid storage on caprine semen conservation at 5 degrees C |
| title_full_unstemmed | Effect of solid storage on caprine semen conservation at 5 degrees C |
| title_short | Effect of solid storage on caprine semen conservation at 5 degrees C |
| title_sort | effect of solid storage on caprine semen conservation at 5 degrees c |
| url | http://hdl.handle.net/20.500.11939/4475 |
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