Rapid detection of cucumber vein yellowing virus by tissue-print hybridisation with digoxigenin-labelled cDNA probes
Hybridisation of tissue prints with nonradioactive cDNA probes was developed to detect cucumber vein yellowing virus (CVYV) in cucurbit plants. Results showed irregular distribution of the virus within cucumber, zucchini or melon plants without defined tropism for a specific tissue. Therefore, relia...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4447 |
| _version_ | 1855032122120601600 |
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| author | Rubio, Luis Janssen, Dirk Cuadrado, I. M. Moreno, Pedro Guerri, José |
| author_browse | Cuadrado, I. M. Guerri, José Janssen, Dirk Moreno, Pedro Rubio, Luis |
| author_facet | Rubio, Luis Janssen, Dirk Cuadrado, I. M. Moreno, Pedro Guerri, José |
| author_sort | Rubio, Luis |
| collection | ReDivia |
| description | Hybridisation of tissue prints with nonradioactive cDNA probes was developed to detect cucumber vein yellowing virus (CVYV) in cucurbit plants. Results showed irregular distribution of the virus within cucumber, zucchini or melon plants without defined tropism for a specific tissue. Therefore, reliable diagnosis of CVYV requires analysis of tissue prints from at least five different plant sites. This detection procedure allows rapid analysis of large numbers of plants and it can be useful for epidemiological studies of CVYV and to control virus spread via eradication of early foci. |
| format | article |
| id | ReDivia4447 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia44472025-04-25T14:43:33Z Rapid detection of cucumber vein yellowing virus by tissue-print hybridisation with digoxigenin-labelled cDNA probes Rubio, Luis Janssen, Dirk Cuadrado, I. M. Moreno, Pedro Guerri, José Hybridisation of tissue prints with nonradioactive cDNA probes was developed to detect cucumber vein yellowing virus (CVYV) in cucurbit plants. Results showed irregular distribution of the virus within cucumber, zucchini or melon plants without defined tropism for a specific tissue. Therefore, reliable diagnosis of CVYV requires analysis of tissue prints from at least five different plant sites. This detection procedure allows rapid analysis of large numbers of plants and it can be useful for epidemiological studies of CVYV and to control virus spread via eradication of early foci. 2017-06-01T10:10:09Z 2017-06-01T10:10:09Z 2003 DEC 2003 article Rubio, L., Janssen, D., Cuadrado, I. M., Moreno, P., Guerri, J. (2003). Rapid detection of cucumber vein yellowing virus by tissue-print hybridisation with digoxigenin-labelled cDNA probes. Journal of virological methods, 114(1), 105-107. 0166-0934 http://hdl.handle.net/20.500.11939/4447 10.1016/j.jviromet.2003.08.008 en openAccess Impreso |
| spellingShingle | Rubio, Luis Janssen, Dirk Cuadrado, I. M. Moreno, Pedro Guerri, José Rapid detection of cucumber vein yellowing virus by tissue-print hybridisation with digoxigenin-labelled cDNA probes |
| title | Rapid detection of cucumber vein yellowing virus by tissue-print hybridisation with digoxigenin-labelled cDNA probes |
| title_full | Rapid detection of cucumber vein yellowing virus by tissue-print hybridisation with digoxigenin-labelled cDNA probes |
| title_fullStr | Rapid detection of cucumber vein yellowing virus by tissue-print hybridisation with digoxigenin-labelled cDNA probes |
| title_full_unstemmed | Rapid detection of cucumber vein yellowing virus by tissue-print hybridisation with digoxigenin-labelled cDNA probes |
| title_short | Rapid detection of cucumber vein yellowing virus by tissue-print hybridisation with digoxigenin-labelled cDNA probes |
| title_sort | rapid detection of cucumber vein yellowing virus by tissue print hybridisation with digoxigenin labelled cdna probes |
| url | http://hdl.handle.net/20.500.11939/4447 |
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