Analysis of the S-locus structure in Prunus armeniaca L. Identification of S-haplotype specific S-RNase and F-box genes
The gametophytic self-incompatibility (GSI) system in Rosaceae has been proposed to be controlled by two genes located in the S-locusan S-RNase and a recently described pollen expressed S-haplotype specific F-box gene (SFB). However, in apricot (Prunus armeniaca L.) these genes had not been identifi...
| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4440 |
| _version_ | 1855032121069928448 |
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| author | Romero, Carlos Vilanova, S. Burgos, Lorenzo Martínez-Calvo, José Vicente, M. Llácer, Gerardo Badenes, María L. |
| author_browse | Badenes, María L. Burgos, Lorenzo Llácer, Gerardo Martínez-Calvo, José Romero, Carlos Vicente, M. Vilanova, S. |
| author_facet | Romero, Carlos Vilanova, S. Burgos, Lorenzo Martínez-Calvo, José Vicente, M. Llácer, Gerardo Badenes, María L. |
| author_sort | Romero, Carlos |
| collection | ReDivia |
| description | The gametophytic self-incompatibility (GSI) system in Rosaceae has been proposed to be controlled by two genes located in the S-locusan S-RNase and a recently described pollen expressed S-haplotype specific F-box gene (SFB). However, in apricot (Prunus armeniaca L.) these genes had not been identified yet. We have sequenced 21kb in total of the S-locus region in 3 different apricot S-haplotypes. These fragments contain genes homologous to the S-RNase and F-box genes found in other Prunus species, preserving their basic gene structure features and defined amino acid domains. The physical distance between the F-boxand the S-RNase genes was determined exactly in the S 2-haplotype (2.9kb) and inferred approximately in the S 1-haplotype (< 49kb) confirming that these genes are linked. Sequence analysis of the 5′ flanking regions indicates the presence of a conserved region upstream of the putative TATA box in the S-RNase gene. The three identified S-RNase alleles (S 1, S 2 and S 4) had a high allelic sequence diversity (75.3 amino acid identity), and the apricot F-box allelic variants (SFB1, SFB2 and SFB4) were also highly haplotype-specific (79.4 amino acid identity). Organ specific-expression was also studied, revealing that S 1- and S 2-RNases are expressed in style tissues, but not in pollen or leaves. In contrast, SFB 1 and SFB 2 are only expressed in pollen, but not in styles or leaves. Taken together, these results support these genes as candidates for the pistil and pollen S-determinants of GSI in apricot. |
| format | article |
| id | ReDivia4440 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia44402025-04-25T14:43:32Z Analysis of the S-locus structure in Prunus armeniaca L. Identification of S-haplotype specific S-RNase and F-box genes Romero, Carlos Vilanova, S. Burgos, Lorenzo Martínez-Calvo, José Vicente, M. Llácer, Gerardo Badenes, María L. The gametophytic self-incompatibility (GSI) system in Rosaceae has been proposed to be controlled by two genes located in the S-locusan S-RNase and a recently described pollen expressed S-haplotype specific F-box gene (SFB). However, in apricot (Prunus armeniaca L.) these genes had not been identified yet. We have sequenced 21kb in total of the S-locus region in 3 different apricot S-haplotypes. These fragments contain genes homologous to the S-RNase and F-box genes found in other Prunus species, preserving their basic gene structure features and defined amino acid domains. The physical distance between the F-boxand the S-RNase genes was determined exactly in the S 2-haplotype (2.9kb) and inferred approximately in the S 1-haplotype (< 49kb) confirming that these genes are linked. Sequence analysis of the 5′ flanking regions indicates the presence of a conserved region upstream of the putative TATA box in the S-RNase gene. The three identified S-RNase alleles (S 1, S 2 and S 4) had a high allelic sequence diversity (75.3 amino acid identity), and the apricot F-box allelic variants (SFB1, SFB2 and SFB4) were also highly haplotype-specific (79.4 amino acid identity). Organ specific-expression was also studied, revealing that S 1- and S 2-RNases are expressed in style tissues, but not in pollen or leaves. In contrast, SFB 1 and SFB 2 are only expressed in pollen, but not in styles or leaves. Taken together, these results support these genes as candidates for the pistil and pollen S-determinants of GSI in apricot. 2017-06-01T10:10:08Z 2017-06-01T10:10:08Z 2004 SEP 2004 article Romero, C., Vilanova, S., Burgos, L., Martinez-Calvo, J., Vicente, M., Llacer, G. et al. (2004). Analysis of the S-locus structure in Prunus armeniaca L. Identification of S-haplotype specific S-RNase and F-box genes. Plant Molecular Biology, 56(1), 145-157. 0167-4412 http://hdl.handle.net/20.500.11939/4440 10.1007/s11103-004-2651-3 en openAccess Impreso |
| spellingShingle | Romero, Carlos Vilanova, S. Burgos, Lorenzo Martínez-Calvo, José Vicente, M. Llácer, Gerardo Badenes, María L. Analysis of the S-locus structure in Prunus armeniaca L. Identification of S-haplotype specific S-RNase and F-box genes |
| title | Analysis of the S-locus structure in Prunus armeniaca L. Identification of S-haplotype specific S-RNase and F-box genes |
| title_full | Analysis of the S-locus structure in Prunus armeniaca L. Identification of S-haplotype specific S-RNase and F-box genes |
| title_fullStr | Analysis of the S-locus structure in Prunus armeniaca L. Identification of S-haplotype specific S-RNase and F-box genes |
| title_full_unstemmed | Analysis of the S-locus structure in Prunus armeniaca L. Identification of S-haplotype specific S-RNase and F-box genes |
| title_short | Analysis of the S-locus structure in Prunus armeniaca L. Identification of S-haplotype specific S-RNase and F-box genes |
| title_sort | analysis of the s locus structure in prunus armeniaca l identification of s haplotype specific s rnase and f box genes |
| url | http://hdl.handle.net/20.500.11939/4440 |
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