Effect of dsRNA on growth rate and reproductive potential of Monosporascus cannonballus
The effect of double stranded RNA (dsRNA) infection on growth rate and the reproductive potential of Monosporascus cannonballus was studied in 21 isolates collected in cucurbit growing areas of Spain and Tunisia. The isolates were incubated on potato dextrose agar (PDA) under different conditions of...
| Main Authors: | , , , , , , , , , |
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| Format: | article |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/4439 |
| _version_ | 1855032120896913408 |
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| author | Armengol, Josep Alaniz, Sandra Vicent, Antonio Beltran, Roberto Abad-Campos, Paloma Pérez-Sierra, Ana M. García-Jiménez, José Ben Salem, Ibtissem Souli, Mounira Boughalleb, Naima |
| author_browse | Abad-Campos, Paloma Alaniz, Sandra Armengol, Josep Beltran, Roberto Ben Salem, Ibtissem Boughalleb, Naima García-Jiménez, José Pérez-Sierra, Ana M. Souli, Mounira Vicent, Antonio |
| author_facet | Armengol, Josep Alaniz, Sandra Vicent, Antonio Beltran, Roberto Abad-Campos, Paloma Pérez-Sierra, Ana M. García-Jiménez, José Ben Salem, Ibtissem Souli, Mounira Boughalleb, Naima |
| author_sort | Armengol, Josep |
| collection | ReDivia |
| description | The effect of double stranded RNA (dsRNA) infection on growth rate and the reproductive potential of Monosporascus cannonballus was studied in 21 isolates collected in cucurbit growing areas of Spain and Tunisia. The isolates were incubated on potato dextrose agar (PDA) under different conditions of temperature, pH, and water potential (Psi(s)). They showed optimal growth temperatures over the range of 27-34 degrees C and perithecia formation was obtained mainly at 25 and 30 degrees C, although some isolates were able to produce perithecia at 35 degrees C. All isolates were able to produce perithecia in a broad range of pHs (4-8). Regarding the effect of Psi(s), the isolates were more tolerant to grow on KCl than on NaCl. For each solute, radial growth decreased progressively as Psi(s) decreased and was severely limited at -5.0 to -6.0 MPa. Perithecia formation was highest at -0.5 MPa, decreased at -1.0 MPa and occurred just in some isolates at -2.0 MPa. Nine of the M. cannonballus isolates harboured dsRNA with 2-6 bands each and a size range of 1.9-18.0 Kb. Phenotypical data were subjected to multivariate factorial analysis. Most of the isolates clustered in two groups corresponding with the presence/absence of dsRNA elements. Isolates without detectable dsRNA produced more perithecia. However, isolates with dsRNA produced lower number of perithecia depending on the pH, Psi(s) or solute used. These results improve our understanding of the behaviour and growth of this pathogen in soil, and can be useful to implement effective disease control. (C) 2011 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. |
| format | article |
| id | ReDivia4439 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia44392025-04-25T14:43:32Z Effect of dsRNA on growth rate and reproductive potential of Monosporascus cannonballus Armengol, Josep Alaniz, Sandra Vicent, Antonio Beltran, Roberto Abad-Campos, Paloma Pérez-Sierra, Ana M. García-Jiménez, José Ben Salem, Ibtissem Souli, Mounira Boughalleb, Naima The effect of double stranded RNA (dsRNA) infection on growth rate and the reproductive potential of Monosporascus cannonballus was studied in 21 isolates collected in cucurbit growing areas of Spain and Tunisia. The isolates were incubated on potato dextrose agar (PDA) under different conditions of temperature, pH, and water potential (Psi(s)). They showed optimal growth temperatures over the range of 27-34 degrees C and perithecia formation was obtained mainly at 25 and 30 degrees C, although some isolates were able to produce perithecia at 35 degrees C. All isolates were able to produce perithecia in a broad range of pHs (4-8). Regarding the effect of Psi(s), the isolates were more tolerant to grow on KCl than on NaCl. For each solute, radial growth decreased progressively as Psi(s) decreased and was severely limited at -5.0 to -6.0 MPa. Perithecia formation was highest at -0.5 MPa, decreased at -1.0 MPa and occurred just in some isolates at -2.0 MPa. Nine of the M. cannonballus isolates harboured dsRNA with 2-6 bands each and a size range of 1.9-18.0 Kb. Phenotypical data were subjected to multivariate factorial analysis. Most of the isolates clustered in two groups corresponding with the presence/absence of dsRNA elements. Isolates without detectable dsRNA produced more perithecia. However, isolates with dsRNA produced lower number of perithecia depending on the pH, Psi(s) or solute used. These results improve our understanding of the behaviour and growth of this pathogen in soil, and can be useful to implement effective disease control. (C) 2011 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. 2017-06-01T10:10:08Z 2017-06-01T10:10:08Z 2011 MAR 2011 article Armengol, J.p, Alaniz, Sandra, Vicent, A., Beltran, Roberto, Abad-Campos, P., Perez-Sierra, Ana, Garcia-Jimenez, J., Ben Salem, Ibtissem, Souli, Mounira, Boughalleb, Naima (2011). Effect of dsRNA on growth rate and reproductive potential of Monosporascus cannonballus. Fungal Biology, 115(3), 236-244. 1878-6146 http://hdl.handle.net/20.500.11939/4439 10.1016/j.funbio.2010.12.007 en openAccess Impreso |
| spellingShingle | Armengol, Josep Alaniz, Sandra Vicent, Antonio Beltran, Roberto Abad-Campos, Paloma Pérez-Sierra, Ana M. García-Jiménez, José Ben Salem, Ibtissem Souli, Mounira Boughalleb, Naima Effect of dsRNA on growth rate and reproductive potential of Monosporascus cannonballus |
| title | Effect of dsRNA on growth rate and reproductive potential of Monosporascus cannonballus |
| title_full | Effect of dsRNA on growth rate and reproductive potential of Monosporascus cannonballus |
| title_fullStr | Effect of dsRNA on growth rate and reproductive potential of Monosporascus cannonballus |
| title_full_unstemmed | Effect of dsRNA on growth rate and reproductive potential of Monosporascus cannonballus |
| title_short | Effect of dsRNA on growth rate and reproductive potential of Monosporascus cannonballus |
| title_sort | effect of dsrna on growth rate and reproductive potential of monosporascus cannonballus |
| url | http://hdl.handle.net/20.500.11939/4439 |
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