Terpene Down-Regulation Triggers Innate Immunity and Resistance to Fungal Pathogens in Orange Fruits
Volatile organic compounds, most of them being terpenes, are signals emitted by plants for communication with the environment. Orange fruit accumulates mainly terpenes in mature peel oil glands with D-limonene accounting for approximately 97% of terpene content. We have generated transgenic orange p...
| Main Authors: | , , , , , , , , , |
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| Other Authors: | |
| Format: | Objeto de conferencia |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/4425 |
| _version_ | 1855491751560609792 |
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| author | Rodriguez, Ana Shimada, Takehiko Cervera, Magdalena Alquezar, Berta Gadea, Jose Gómez-Cadenas, Aurelio De-Ollas, Carlos J. Rodrigo, María J. Zacarias, Lorenzo Pena, Leandro |
| author2 | Sabater-Munoz, Beatriz |
| author_browse | Alquezar, Berta Cervera, Magdalena De-Ollas, Carlos J. Gadea, Jose Gómez-Cadenas, Aurelio Pena, Leandro Rodrigo, María J. Rodriguez, Ana Sabater-Munoz, Beatriz Shimada, Takehiko Zacarias, Lorenzo |
| author_facet | Sabater-Munoz, Beatriz Rodriguez, Ana Shimada, Takehiko Cervera, Magdalena Alquezar, Berta Gadea, Jose Gómez-Cadenas, Aurelio De-Ollas, Carlos J. Rodrigo, María J. Zacarias, Lorenzo Pena, Leandro |
| author_sort | Rodriguez, Ana |
| collection | ReDivia |
| description | Volatile organic compounds, most of them being terpenes, are signals emitted by plants for communication with the environment. Orange fruit accumulates mainly terpenes in mature peel oil glands with D-limonene accounting for approximately 97% of terpene content. We have generated transgenic orange plants carrying a D-limonene synthase gene in antisense (AS) configuration. Transgenic expression caused a dramatic decrease in the accumulation of D-limonene in fruit peels, being about 80-100 times lower in AS samples than in empty vector (EV) transgenic ones. This affected the interaction of fruits with their biotic environment because they resulted in resistance to different specialized pathogens. When antisense fruits were challenged with the fungus Penicillium digitatum, they showed marked resistance against this pathogen. A gene expression analysis of these fruits linked the decrease of D-limonene to upregulation of genes involved in the innate immunity response. High D-limonene content in mature orange peels may be a signal for attractiveness of microorganisms which might be likely involved in facilitating the access to the pulp of seed dispersal frugivores. |
| format | Objeto de conferencia |
| id | ReDivia4425 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia44252025-04-25T14:52:23Z Terpene Down-Regulation Triggers Innate Immunity and Resistance to Fungal Pathogens in Orange Fruits Acta Horticulturae Rodriguez, Ana Shimada, Takehiko Cervera, Magdalena Alquezar, Berta Gadea, Jose Gómez-Cadenas, Aurelio De-Ollas, Carlos J. Rodrigo, María J. Zacarias, Lorenzo Pena, Leandro Sabater-Munoz, Beatriz Moreno, Pedro Pena, Leandro Navarro, Luis Volatile organic compounds, most of them being terpenes, are signals emitted by plants for communication with the environment. Orange fruit accumulates mainly terpenes in mature peel oil glands with D-limonene accounting for approximately 97% of terpene content. We have generated transgenic orange plants carrying a D-limonene synthase gene in antisense (AS) configuration. Transgenic expression caused a dramatic decrease in the accumulation of D-limonene in fruit peels, being about 80-100 times lower in AS samples than in empty vector (EV) transgenic ones. This affected the interaction of fruits with their biotic environment because they resulted in resistance to different specialized pathogens. When antisense fruits were challenged with the fungus Penicillium digitatum, they showed marked resistance against this pathogen. A gene expression analysis of these fruits linked the decrease of D-limonene to upregulation of genes involved in the innate immunity response. High D-limonene content in mature orange peels may be a signal for attractiveness of microorganisms which might be likely involved in facilitating the access to the pulp of seed dispersal frugivores. 2017-06-01T10:10:06Z 2017-06-01T10:10:06Z 2015 2015 conferenceObject Rodriguez, A., Shimada, Takehiko, Cervera, M., Alquezar, B., Gadea, J., Gomez-Cadenas, Aurelio, De Ollas, C. J., Rodrigo, M.J., Zacarias, L., Pena, L. (2015). Terpene Down-Regulation Triggers Innate Immunity and Resistance to Fungal Pathogens in Orange Fruits. Acta Horticulturae, 1065, 687-693. 0567-7572; 978-94-62610-53-8 http://hdl.handle.net/20.500.11939/4425 10.17660/ActaHortic.2015.1065.86 en openAccess Impreso |
| spellingShingle | Rodriguez, Ana Shimada, Takehiko Cervera, Magdalena Alquezar, Berta Gadea, Jose Gómez-Cadenas, Aurelio De-Ollas, Carlos J. Rodrigo, María J. Zacarias, Lorenzo Pena, Leandro Terpene Down-Regulation Triggers Innate Immunity and Resistance to Fungal Pathogens in Orange Fruits |
| title | Terpene Down-Regulation Triggers Innate Immunity and Resistance to Fungal Pathogens in Orange Fruits |
| title_full | Terpene Down-Regulation Triggers Innate Immunity and Resistance to Fungal Pathogens in Orange Fruits |
| title_fullStr | Terpene Down-Regulation Triggers Innate Immunity and Resistance to Fungal Pathogens in Orange Fruits |
| title_full_unstemmed | Terpene Down-Regulation Triggers Innate Immunity and Resistance to Fungal Pathogens in Orange Fruits |
| title_short | Terpene Down-Regulation Triggers Innate Immunity and Resistance to Fungal Pathogens in Orange Fruits |
| title_sort | terpene down regulation triggers innate immunity and resistance to fungal pathogens in orange fruits |
| url | http://hdl.handle.net/20.500.11939/4425 |
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