Terpene Down-Regulation in Orange Reveals the Role of Fruit Aromas in Mediating Interactions with Insect Herbivores and Pathogens
Plants use volatile terpene compounds as odor cues for communicating with the environment. Fleshy fruits are particularly rich in volatiles that deter herbivores and attract seed dispersal agents. We have investigated how terpenes in citrus fruit peels affect the interaction between the plant, insec...
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Acceso en línea: | http://hdl.handle.net/20.500.11939/4423 |
| _version_ | 1855491751174733824 |
|---|---|
| author | Rodriguez, Ana San-Andrés, Victoria Cervera, Magdalena Redondo, Ana Alquezar, Berta Shimada, Takehiko Gadea, Jose Rodrigo, María J. Zacarias, Lorenzo Palou, Lluís López, María M. Castanera, Pedro Pena, Leandro |
| author_browse | Alquezar, Berta Castanera, Pedro Cervera, Magdalena Gadea, Jose López, María M. Palou, Lluís Pena, Leandro Redondo, Ana Rodrigo, María J. Rodriguez, Ana San-Andrés, Victoria Shimada, Takehiko Zacarias, Lorenzo |
| author_facet | Rodriguez, Ana San-Andrés, Victoria Cervera, Magdalena Redondo, Ana Alquezar, Berta Shimada, Takehiko Gadea, Jose Rodrigo, María J. Zacarias, Lorenzo Palou, Lluís López, María M. Castanera, Pedro Pena, Leandro |
| author_sort | Rodriguez, Ana |
| collection | ReDivia |
| description | Plants use volatile terpene compounds as odor cues for communicating with the environment. Fleshy fruits are particularly rich in volatiles that deter herbivores and attract seed dispersal agents. We have investigated how terpenes in citrus fruit peels affect the interaction between the plant, insects, and microorganisms. Because limonene represents up to 97% of the total volatiles in orange (Citrus sinensis) fruit peel, we chose to down-regulate the expression of a limonene synthase gene in orange plants by introducing an antisense construct of this gene. Transgenic fruits showed reduced accumulation of limonene in the peel. When these fruits were challenged with either the fungus Penicillium digitatum or with the bacterium Xanthomonas citri subsp. citri, they showed marked resistance against these pathogens that were unable to infect the peel tissues. Moreover, males of the citrus pest medfly (Ceratitis capitata) were less attracted to low limonene-expressing fruits than to control fruits. These results indicate that limonene accumulation in the peel of citrus fruit appears to be involved in the successful trophic interaction between fruits, insects, and microorganisms. Terpene down-regulation might be a strategy to generate broad-spectrum resistance against pests and pathogens in fleshy fruits from economically important crops. In addition, terpene engineering may be important for studying the basic ecological interactions between fruits, herbivores, and pathogens. |
| format | Artículo |
| id | ReDivia4423 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia44232025-04-25T14:43:29Z Terpene Down-Regulation in Orange Reveals the Role of Fruit Aromas in Mediating Interactions with Insect Herbivores and Pathogens Rodriguez, Ana San-Andrés, Victoria Cervera, Magdalena Redondo, Ana Alquezar, Berta Shimada, Takehiko Gadea, Jose Rodrigo, María J. Zacarias, Lorenzo Palou, Lluís López, María M. Castanera, Pedro Pena, Leandro Plants use volatile terpene compounds as odor cues for communicating with the environment. Fleshy fruits are particularly rich in volatiles that deter herbivores and attract seed dispersal agents. We have investigated how terpenes in citrus fruit peels affect the interaction between the plant, insects, and microorganisms. Because limonene represents up to 97% of the total volatiles in orange (Citrus sinensis) fruit peel, we chose to down-regulate the expression of a limonene synthase gene in orange plants by introducing an antisense construct of this gene. Transgenic fruits showed reduced accumulation of limonene in the peel. When these fruits were challenged with either the fungus Penicillium digitatum or with the bacterium Xanthomonas citri subsp. citri, they showed marked resistance against these pathogens that were unable to infect the peel tissues. Moreover, males of the citrus pest medfly (Ceratitis capitata) were less attracted to low limonene-expressing fruits than to control fruits. These results indicate that limonene accumulation in the peel of citrus fruit appears to be involved in the successful trophic interaction between fruits, insects, and microorganisms. Terpene down-regulation might be a strategy to generate broad-spectrum resistance against pests and pathogens in fleshy fruits from economically important crops. In addition, terpene engineering may be important for studying the basic ecological interactions between fruits, herbivores, and pathogens. 2017-06-01T10:10:05Z 2017-06-01T10:10:05Z 2011 JUN 2011 article Rodriguez, A., San Andres, Victoria, Cervera, M., Redondo, A., Alquezar, B., Shimada, Takehiko, Gadea, J., Jesus Rodrigo, M., Zacarias, L., Palou, L., Lopez, M.M., Castanera, P., Pena, L. (2011). Terpene Down-Regulation in Orange Reveals the Role of Fruit Aromas in Mediating Interactions with Insect Herbivores and Pathogens. Plant Physiology, 156(2), 793-802. 0032-0889 http://hdl.handle.net/20.500.11939/4423 10.1104/pp.111.176545 en openAccess Impreso |
| spellingShingle | Rodriguez, Ana San-Andrés, Victoria Cervera, Magdalena Redondo, Ana Alquezar, Berta Shimada, Takehiko Gadea, Jose Rodrigo, María J. Zacarias, Lorenzo Palou, Lluís López, María M. Castanera, Pedro Pena, Leandro Terpene Down-Regulation in Orange Reveals the Role of Fruit Aromas in Mediating Interactions with Insect Herbivores and Pathogens |
| title | Terpene Down-Regulation in Orange Reveals the Role of Fruit Aromas in Mediating Interactions with Insect Herbivores and Pathogens |
| title_full | Terpene Down-Regulation in Orange Reveals the Role of Fruit Aromas in Mediating Interactions with Insect Herbivores and Pathogens |
| title_fullStr | Terpene Down-Regulation in Orange Reveals the Role of Fruit Aromas in Mediating Interactions with Insect Herbivores and Pathogens |
| title_full_unstemmed | Terpene Down-Regulation in Orange Reveals the Role of Fruit Aromas in Mediating Interactions with Insect Herbivores and Pathogens |
| title_short | Terpene Down-Regulation in Orange Reveals the Role of Fruit Aromas in Mediating Interactions with Insect Herbivores and Pathogens |
| title_sort | terpene down regulation in orange reveals the role of fruit aromas in mediating interactions with insect herbivores and pathogens |
| url | http://hdl.handle.net/20.500.11939/4423 |
| work_keys_str_mv | AT rodriguezana terpenedownregulationinorangerevealstheroleoffruitaromasinmediatinginteractionswithinsectherbivoresandpathogens AT sanandresvictoria terpenedownregulationinorangerevealstheroleoffruitaromasinmediatinginteractionswithinsectherbivoresandpathogens AT cerveramagdalena terpenedownregulationinorangerevealstheroleoffruitaromasinmediatinginteractionswithinsectherbivoresandpathogens AT redondoana terpenedownregulationinorangerevealstheroleoffruitaromasinmediatinginteractionswithinsectherbivoresandpathogens AT alquezarberta terpenedownregulationinorangerevealstheroleoffruitaromasinmediatinginteractionswithinsectherbivoresandpathogens AT shimadatakehiko terpenedownregulationinorangerevealstheroleoffruitaromasinmediatinginteractionswithinsectherbivoresandpathogens AT gadeajose terpenedownregulationinorangerevealstheroleoffruitaromasinmediatinginteractionswithinsectherbivoresandpathogens AT rodrigomariaj terpenedownregulationinorangerevealstheroleoffruitaromasinmediatinginteractionswithinsectherbivoresandpathogens AT zacariaslorenzo terpenedownregulationinorangerevealstheroleoffruitaromasinmediatinginteractionswithinsectherbivoresandpathogens AT paloulluis terpenedownregulationinorangerevealstheroleoffruitaromasinmediatinginteractionswithinsectherbivoresandpathogens AT lopezmariam terpenedownregulationinorangerevealstheroleoffruitaromasinmediatinginteractionswithinsectherbivoresandpathogens AT castanerapedro terpenedownregulationinorangerevealstheroleoffruitaromasinmediatinginteractionswithinsectherbivoresandpathogens AT penaleandro terpenedownregulationinorangerevealstheroleoffruitaromasinmediatinginteractionswithinsectherbivoresandpathogens |