The Citrus leaf blotch virus movement protein acts as silencing suppressor
To counteract plant antiviral defense based on RNA silencing, many viruses express proteins that inhibit this mechanism at different levels. The genome of Citrus leaf blotch virus (CLBV) encodes a 227-kDa protein involved in replication, a 40-kDa movement protein (MP), and a 41-kDa coat protein (CP)...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/4408 |
| _version_ | 1855491747707092992 |
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| author | Renovell, Agueda Vives, María C. Ruiz-Ruiz, Susana Navarro, Luis Moreno, Pedro Guerri, José |
| author_browse | Guerri, José Moreno, Pedro Navarro, Luis Renovell, Agueda Ruiz-Ruiz, Susana Vives, María C. |
| author_facet | Renovell, Agueda Vives, María C. Ruiz-Ruiz, Susana Navarro, Luis Moreno, Pedro Guerri, José |
| author_sort | Renovell, Agueda |
| collection | ReDivia |
| description | To counteract plant antiviral defense based on RNA silencing, many viruses express proteins that inhibit this mechanism at different levels. The genome of Citrus leaf blotch virus (CLBV) encodes a 227-kDa protein involved in replication, a 40-kDa movement protein (MP), and a 41-kDa coat protein (CP). To determine if any of these proteins might have RNA silencing suppressor activities, we have used Agrobacterium-mediated transient assays in the green fluorescent protein (GFP)-expressing Nicotiana benthamiana line 16c. Only CLBV MP was able to suppress intracellular GFP silencing induced by expression of either single- or double-stranded (ds) GFP RNA, but not cell-to-cell or long distance spread of the silencing signal. The MP suppressor activity was weak compared to other characterized viral suppressor proteins. Overall our data indicate that MP acts as a suppressor of local silencing probably by interfering in the silencing pathway downstream of the steps of dsRNA and small RNAs generation. |
| format | Artículo |
| id | ReDivia4408 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia44082025-04-25T14:43:04Z The Citrus leaf blotch virus movement protein acts as silencing suppressor Renovell, Agueda Vives, María C. Ruiz-Ruiz, Susana Navarro, Luis Moreno, Pedro Guerri, José To counteract plant antiviral defense based on RNA silencing, many viruses express proteins that inhibit this mechanism at different levels. The genome of Citrus leaf blotch virus (CLBV) encodes a 227-kDa protein involved in replication, a 40-kDa movement protein (MP), and a 41-kDa coat protein (CP). To determine if any of these proteins might have RNA silencing suppressor activities, we have used Agrobacterium-mediated transient assays in the green fluorescent protein (GFP)-expressing Nicotiana benthamiana line 16c. Only CLBV MP was able to suppress intracellular GFP silencing induced by expression of either single- or double-stranded (ds) GFP RNA, but not cell-to-cell or long distance spread of the silencing signal. The MP suppressor activity was weak compared to other characterized viral suppressor proteins. Overall our data indicate that MP acts as a suppressor of local silencing probably by interfering in the silencing pathway downstream of the steps of dsRNA and small RNAs generation. 2017-06-01T10:10:03Z 2017-06-01T10:10:03Z 2012 FEB 2012 article Renovell, A., Vives, M., Ruiz-Ruiz, S., Navarro, L., Moreno, P. & Guerri, J. (2012). The Citrus leaf blotch virus movement protein acts as silencing suppressor. Virus genes, 44(1), 131-140. 0920-8569 http://hdl.handle.net/20.500.11939/4408 10.1007/s11262-011-0674-9 en openAccess Impreso |
| spellingShingle | Renovell, Agueda Vives, María C. Ruiz-Ruiz, Susana Navarro, Luis Moreno, Pedro Guerri, José The Citrus leaf blotch virus movement protein acts as silencing suppressor |
| title | The Citrus leaf blotch virus movement protein acts as silencing suppressor |
| title_full | The Citrus leaf blotch virus movement protein acts as silencing suppressor |
| title_fullStr | The Citrus leaf blotch virus movement protein acts as silencing suppressor |
| title_full_unstemmed | The Citrus leaf blotch virus movement protein acts as silencing suppressor |
| title_short | The Citrus leaf blotch virus movement protein acts as silencing suppressor |
| title_sort | citrus leaf blotch virus movement protein acts as silencing suppressor |
| url | http://hdl.handle.net/20.500.11939/4408 |
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