IS53: an insertion element for molecular typing of Pseudomonas savastanoi pv. savastanoi
A worldwide collection of Pseudomonas savastanoi pv. savastanoi strains from olive knots was examined for the distribution, variation in position and copy numbers of the IS53 insertion element. Southern hybridization analysis of plasmid DNA from six olive strains using IS53 and repA probes revealed...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4388 |
| _version_ | 1855491744675659776 |
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| author | Quesada, Jose M. Perez-Martinez, Isabel Ramos, Cayo López, María M. Penyalver, Ramón |
| author_browse | López, María M. Penyalver, Ramón Perez-Martinez, Isabel Quesada, Jose M. Ramos, Cayo |
| author_facet | Quesada, Jose M. Perez-Martinez, Isabel Ramos, Cayo López, María M. Penyalver, Ramón |
| author_sort | Quesada, Jose M. |
| collection | ReDivia |
| description | A worldwide collection of Pseudomonas savastanoi pv. savastanoi strains from olive knots was examined for the distribution, variation in position and copy numbers of the IS53 insertion element. Southern hybridization analysis of plasmid DNA from six olive strains using IS53 and repA probes revealed that this insertion element was present in the chromosomal replicon and not in a plasmid, as had been originally described in an oleander strain. Southern hybridization analysis also revealed that IS53 was present in multiple copies in all analyzed strains. Copy numbers of IS53 elements ranged from 4 up to 10. Although all strains displayed a remarkably high degree of restriction fragment length polymorphism, we demonstrated that transposition of this element is extremely rare in bacteria grown in vitro for up to 390 generations. The genetic diversity of 62 olive strains based on 47 different IS53 RFLP fingerprints and UPGMA analysis enabled all strains to be clustered into eight groups with 60% similarity. IS53 typing can be considered a suitable marker for epidemiological and ecological studies, given its widespread distribution on P. savastanoi pv. savastanoi olive populations, its high stability and the high degree of polymorphism generated. |
| format | Artículo |
| id | ReDivia4388 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia43882025-04-25T14:43:01Z IS53: an insertion element for molecular typing of Pseudomonas savastanoi pv. savastanoi Quesada, Jose M. Perez-Martinez, Isabel Ramos, Cayo López, María M. Penyalver, Ramón A worldwide collection of Pseudomonas savastanoi pv. savastanoi strains from olive knots was examined for the distribution, variation in position and copy numbers of the IS53 insertion element. Southern hybridization analysis of plasmid DNA from six olive strains using IS53 and repA probes revealed that this insertion element was present in the chromosomal replicon and not in a plasmid, as had been originally described in an oleander strain. Southern hybridization analysis also revealed that IS53 was present in multiple copies in all analyzed strains. Copy numbers of IS53 elements ranged from 4 up to 10. Although all strains displayed a remarkably high degree of restriction fragment length polymorphism, we demonstrated that transposition of this element is extremely rare in bacteria grown in vitro for up to 390 generations. The genetic diversity of 62 olive strains based on 47 different IS53 RFLP fingerprints and UPGMA analysis enabled all strains to be clustered into eight groups with 60% similarity. IS53 typing can be considered a suitable marker for epidemiological and ecological studies, given its widespread distribution on P. savastanoi pv. savastanoi olive populations, its high stability and the high degree of polymorphism generated. 2017-06-01T10:10:00Z 2017-06-01T10:10:00Z 2008 APR 2008 article Quesada, J.M., Perez-Martinez, I., Ramos, C., Lopez, M.M., Penyalver, R. (2008). IS53: an insertion element for molecular typing of Pseudomonas savastanoi pv. savastanoi. Research in microbiology, 159(3), 207-215. 0923-2508 http://hdl.handle.net/20.500.11939/4388 10.1016/j.resmic.2007.12.010 en openAccess Impreso |
| spellingShingle | Quesada, Jose M. Perez-Martinez, Isabel Ramos, Cayo López, María M. Penyalver, Ramón IS53: an insertion element for molecular typing of Pseudomonas savastanoi pv. savastanoi |
| title | IS53: an insertion element for molecular typing of Pseudomonas savastanoi pv. savastanoi |
| title_full | IS53: an insertion element for molecular typing of Pseudomonas savastanoi pv. savastanoi |
| title_fullStr | IS53: an insertion element for molecular typing of Pseudomonas savastanoi pv. savastanoi |
| title_full_unstemmed | IS53: an insertion element for molecular typing of Pseudomonas savastanoi pv. savastanoi |
| title_short | IS53: an insertion element for molecular typing of Pseudomonas savastanoi pv. savastanoi |
| title_sort | is53 an insertion element for molecular typing of pseudomonas savastanoi pv savastanoi |
| url | http://hdl.handle.net/20.500.11939/4388 |
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