Assessment of Pollen-Mediated Transgene Flow in Citrus under Experimental Field Conditions
Despite potential benefits granted by genetically modified (GM) citrus trees, their release and commercialization raises concerns about their potential environmental impact. The transfer via pollen of transgenes to cross-compatible cultivars is deemed to be the greatest source for environmental expo...
| Autores principales: | , , , |
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| Otros Autores: | |
| Formato: | Objeto de conferencia |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4378 |
| _version_ | 1855491742729502720 |
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| author | Pons, Elsa Navarro, Antonio Ollitrault, Patrick Pena, Leandro |
| author2 | Sabater-Munoz, Beatriz |
| author_browse | Navarro, Antonio Ollitrault, Patrick Pena, Leandro Pons, Elsa Sabater-Munoz, Beatriz |
| author_facet | Sabater-Munoz, Beatriz Pons, Elsa Navarro, Antonio Ollitrault, Patrick Pena, Leandro |
| author_sort | Pons, Elsa |
| collection | ReDivia |
| description | Despite potential benefits granted by genetically modified (GM) citrus trees, their release and commercialization raises concerns about their potential environmental impact. The transfer via pollen of transgenes to cross-compatible cultivars is deemed to be the greatest source for environmental exposure. In this work, three different citrus genotypes carrying the uidA (GUS) tracer marker gene (pollen donors) and a non-GM self-incompatible contiguous citrus genotype (recipient) were used in conditions allowing natural entomophilous pollination to occur. The examination of 603 to 2990 seeds per year showed unexpectedly low frequencies (0.17-2.86%) of transgene flow. Paternity analyses of the progeny of subsets of recipient plants using 10 microsatellite (SSR) loci demonstrated a higher mating competence of trees from another non-GM pollen source population that greatly limited the mating chance of the contiguous cross-compatible and flowering-synchronized transgenic pollen source. This mating superiority could be explained by much higher pollen competition capacity of the non-GM genotypes, as was confirmed through mixed-hand pollinations, indicating that pollen competition strongly contributed to transgene confinement. This is the first study on transgene flow in citrus. It provides crucial information on the safety and field performance of GM citrus that can serve as a basis for further field trials and as a guide for (case-by-case) regulatory policies. |
| format | Objeto de conferencia |
| id | ReDivia4378 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia43782025-04-25T14:52:31Z Assessment of Pollen-Mediated Transgene Flow in Citrus under Experimental Field Conditions Acta Horticulturae Pons, Elsa Navarro, Antonio Ollitrault, Patrick Pena, Leandro Sabater-Munoz, Beatriz Moreno, Pedro Pena, Leandro Navarro, Luis Despite potential benefits granted by genetically modified (GM) citrus trees, their release and commercialization raises concerns about their potential environmental impact. The transfer via pollen of transgenes to cross-compatible cultivars is deemed to be the greatest source for environmental exposure. In this work, three different citrus genotypes carrying the uidA (GUS) tracer marker gene (pollen donors) and a non-GM self-incompatible contiguous citrus genotype (recipient) were used in conditions allowing natural entomophilous pollination to occur. The examination of 603 to 2990 seeds per year showed unexpectedly low frequencies (0.17-2.86%) of transgene flow. Paternity analyses of the progeny of subsets of recipient plants using 10 microsatellite (SSR) loci demonstrated a higher mating competence of trees from another non-GM pollen source population that greatly limited the mating chance of the contiguous cross-compatible and flowering-synchronized transgenic pollen source. This mating superiority could be explained by much higher pollen competition capacity of the non-GM genotypes, as was confirmed through mixed-hand pollinations, indicating that pollen competition strongly contributed to transgene confinement. This is the first study on transgene flow in citrus. It provides crucial information on the safety and field performance of GM citrus that can serve as a basis for further field trials and as a guide for (case-by-case) regulatory policies. 2017-06-01T10:09:59Z 2017-06-01T10:09:59Z 2015 2015 conferenceObject Pons, E., Navarro, A., Ollitrault, P., Pena, L. (2015). Assessment of Pollen-Mediated Transgene Flow in Citrus under Experimental Field Conditions. Acta Horticulturae, 1065, 711-718. 0567-7572; 978-94-62610-53-8 http://hdl.handle.net/20.500.11939/4378 10.17660/ActaHortic.2015.1065.89 en openAccess Impreso |
| spellingShingle | Pons, Elsa Navarro, Antonio Ollitrault, Patrick Pena, Leandro Assessment of Pollen-Mediated Transgene Flow in Citrus under Experimental Field Conditions |
| title | Assessment of Pollen-Mediated Transgene Flow in Citrus under Experimental Field Conditions |
| title_full | Assessment of Pollen-Mediated Transgene Flow in Citrus under Experimental Field Conditions |
| title_fullStr | Assessment of Pollen-Mediated Transgene Flow in Citrus under Experimental Field Conditions |
| title_full_unstemmed | Assessment of Pollen-Mediated Transgene Flow in Citrus under Experimental Field Conditions |
| title_short | Assessment of Pollen-Mediated Transgene Flow in Citrus under Experimental Field Conditions |
| title_sort | assessment of pollen mediated transgene flow in citrus under experimental field conditions |
| url | http://hdl.handle.net/20.500.11939/4378 |
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