Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars
Nucellus-derived embryogenic callus cultures of Salustiana sweet orange were subjected to cryoconservation assays, Cryoprotection with 10%(vol/vol) dimethylsulfoxide, freezing by slow cooling and thawing by ast warming was suitable to recover viable growing cultures and whole plants through embryoge...
| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4355 |
| _version_ | 1855032106840752128 |
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| author | Perez, Rosa Navarro, Luis Durán-Vila, Núria |
| author_browse | Durán-Vila, Núria Navarro, Luis Perez, Rosa |
| author_facet | Perez, Rosa Navarro, Luis Durán-Vila, Núria |
| author_sort | Perez, Rosa |
| collection | ReDivia |
| description | Nucellus-derived embryogenic callus cultures of Salustiana sweet orange were subjected to cryoconservation assays, Cryoprotection with 10%(vol/vol) dimethylsulfoxide, freezing by slow cooling and thawing by ast warming was suitable to recover viable growing cultures and whole plants through embryogenesis. Evaluation of liquid phase R-1 and solid phase R-2 cooling rates using a programmable freezing unit indicated that 100% of embryogenic cultures survived when frozen using a range of cooling rates (R-1 not above 0.5 degrees C min(-1) and R-2 not above 1 degrees C min(1)) and thawed by fast warming. Storage up to 2 years in liquid nitrogen did not affect the growth of the cryopreserved cultures and the recovery of whole plants. Cultures of four cultivars of sweet orange (C, sinensis Osb.), three cultivars of grapefruit (C, paradisi Macf,), and one cultivar each of lemon [C. limon (L.) Burm. f.], Cleopatra mandarin (C. reshni Hort, ex Tan.), sour orange (C. aurantium L.) and Mexican lime [C. aurantifolia (Christm.) Swing.] have been successfully cryopreserved. Problems using a viability assessment using fluorescein diacetate staining are discussed. |
| format | article |
| id | ReDivia4355 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia43552025-04-25T14:42:56Z Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars Perez, Rosa Navarro, Luis Durán-Vila, Núria Nucellus-derived embryogenic callus cultures of Salustiana sweet orange were subjected to cryoconservation assays, Cryoprotection with 10%(vol/vol) dimethylsulfoxide, freezing by slow cooling and thawing by ast warming was suitable to recover viable growing cultures and whole plants through embryogenesis. Evaluation of liquid phase R-1 and solid phase R-2 cooling rates using a programmable freezing unit indicated that 100% of embryogenic cultures survived when frozen using a range of cooling rates (R-1 not above 0.5 degrees C min(-1) and R-2 not above 1 degrees C min(1)) and thawed by fast warming. Storage up to 2 years in liquid nitrogen did not affect the growth of the cryopreserved cultures and the recovery of whole plants. Cultures of four cultivars of sweet orange (C, sinensis Osb.), three cultivars of grapefruit (C, paradisi Macf,), and one cultivar each of lemon [C. limon (L.) Burm. f.], Cleopatra mandarin (C. reshni Hort, ex Tan.), sour orange (C. aurantium L.) and Mexican lime [C. aurantifolia (Christm.) Swing.] have been successfully cryopreserved. Problems using a viability assessment using fluorescein diacetate staining are discussed. 2017-06-01T10:09:55Z 2017-06-01T10:09:55Z 1997 NOV article Perez, R., Navarro, L. & Duran-Vila, N. (1997). Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars. Plant Cell Reports, 17(1), 44-49. 0721-7714 http://hdl.handle.net/20.500.11939/4355 10.1007/s002990050349 en openAccess Impreso |
| spellingShingle | Perez, Rosa Navarro, Luis Durán-Vila, Núria Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars |
| title | Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars |
| title_full | Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars |
| title_fullStr | Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars |
| title_full_unstemmed | Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars |
| title_short | Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars |
| title_sort | cryopreservation and storage of embryogenic callus cultures of several citrus species and cultivars |
| url | http://hdl.handle.net/20.500.11939/4355 |
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